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Biomedical subjects

E Gonzalez

Publications and source records attributed to E Gonzalez.

212 records · Page 12Linked to original sources
PubMedDate not supplied

A simulation of three-dimensional systolic flow dynamics in a spherical ventricle: effects of abnormal wall motion.

Alterations in left ventricle (LV) wall motion induced by ischemia will affect flow dynamics, and these altered flow fields can be used to evaluate LV pumping efficiency. LV chamber flow fields were obtained in this study by solving the discretized three-dimensional Navier-Stokes equations for viscous, incompressible unsteady flow by using the finite analytic method. Several cases of abnormal wall motion (AWM) were simulated by a manipulation of the boundary conditions to produce regions of hypokinesis, akinesis, and dyskinesis. These solutions were used to determine the central ejection region (CER), defined as the region of flow domain in which the obtained velocity field vectors are aligned +/- 3 degrees from the LV long axis. A CER coefficient was computed from information on the location and orientation of the CER within the LV cavity. Contraction of the spherical ventricle produced a vector field that was symmetric with respect to the long axis. For the simulations of AWM, an asymmetrical flow pattern developed, became more pronounced with increasing severity of AWM, and resulted in a shorter CER that shifted toward the ischemic region. The CER coefficients decreased monotonically with increased severity in AWM from 0.948 in the normal case to a low of 0.164 for the most severe case of AWM. The CER coefficient quantitatively displayed the sensitivity of the flow patterns to even moderate degrees of hypokinesis. In addition, visualization of the three-dimensional flow field reinforced the necessity of three-dimensional simulations to capture aspects of the flow that existing methods of two-dimensional flow imaging that use ultrasound may miss.

Computer Simulation↗
PubMedDate not supplied

Seatbelt use and belief in destiny among Hispanic and non-Hispanic drivers.

In order to determine if fatalism is associated with seatbelt use, 312 drivers were recruited from convenience stores located within 12 randomly generated zip-code areas along the Texas-Mexico border. The seatbelt use of drivers was recorded unobtrusively; self-reported seatbelt use and belief in destiny (fatalism) were subsequently obtained by interview. The majority of respondents rejected a fatalistic orientation, although Hispanic drivers were significantly less likely to do so. Fatalism was not associated with observed seatbelt use. Our results suggest that ethnic differences in seatbelt use are not due to ethnic differences in fatalism. These findings have implications for developing health interventions in Hispanic and non-Hispanic communities.

Adolescent↗
PubMedDate not supplied

Detection and classification of Trypanosoma cruzi by DNA hybridization with nonradioactive probes.

Total or kinetoplast DNA (kDNA) from 72 isolates and clones of Trypanosoma cruzi as well as from nine related trypanosomatids were analyzed by dot hybridization using nonradioactive kDNA or cloned minicircle fragments as probes. Biotinylated-kDNA probes generated by nick-translation proved reliable for distinguishing Zymodeme 1 and Zymodeme 2bol of T. cruzi parasites. In contrast, digoxigenin-labeled kDNA obtained by random-priming did not distinguish among T. cruzi isolates but did distinguish among New World leishmanias. Cloned minicircle fragments labeled with digoxigenin gave the same results as digoxigenin-labeled kDNA, except for a 10-fold decrease in sensitivity. Digoxigenin-labeled DNA probes proved useful in unambiguously detecting T. cruzi from different geographic regions of America. However, T. rangeli and T. cruzi marinkellei were not distinguished by these probes.

Animals↗
PubMedDate not supplied

The feasibility of forecasting influenza epidemics in Cuba.

A large influenza epidemic took place in Havana during the winter of 1988. The epidemiologic surveillance unit of the Pedro Kourí Institute of Tropical Medicine detected the beginning of the epidemic wave. The Rvachev-Baroyan mathematical model of the geographic spread of an epidemic was used to forecast this epidemic under routine conditions of the public health system. The expected number of individuals who would attend outpatient services, because of influenza-like illness, was calculated and communicated to the health authorities within enough time to permit the introduction of available control measures. The approximate date of the epidemic peak, the daily expected number of individuals attending medical services, and the approximate time of the end of the epidemic wave were estimated. The prediction error was 12%. The model was sufficiently accurate to warrant its use as a practical forecasting tool in the Cuban public health system.

Cuba↗
PubMedDate not supplied

Forecast of acute respiratory infections: expected nonepidemic morbidity in Cuba.

A forecast of nonepidemic morbidity due to acute respiratory infections were carry out by using time series analysis. The data consisted of the weekly reports of medical patient consultation from ambulatory facilities from the whole country. A version of regression model was fitted to the data. Using this approach, we were able to detect the starting data of the epidemic under routine surveillance conditions for various age groups. It will be necessary to improve the data reporting system in order to introduce these procedures at the local health center level, as well as on the provincial level.

Adolescent↗
PubMedDate not supplied

Agreement between paramedic-estimated weights and subsequent hospital measurements in adults with out-of-hospital cardiac arrest.

OBJECTIVE: To assess the accuracy of paramedic estimates of adult body weights in cardiac arrest cases. HYPOTHESIS: Paramedics could accurately estimate the weights of out-of-hospital cardiac arrest patients. DESIGN: Retrospective data analysis of a 15-month, multicenter study involving nontraumatic out-of-hospital cardiac arrest patients. Paramedic estimates of body weights were compared to weights measured in the hospital. Patients were included in the analysis only if both a paramedic weight and a measured in-hospital weight were recorded. SETTING: Six urban emergency medical services systems. PARTICIPANTS: The study population included adults with return of spontaneous circulation who subsequently were admitted to the hospital. MEASUREMENTS: Pearson correlation analysis of paramedic-estimated weights and measured weights. RESULTS: Among the 133 study patients, the correlation coefficient (R) for paramedic estimates and the actual measured weight was 0.93. Paramedic estimates of weight were within 10% of the measured weights in 74% of the patients, and within 20% of measured weights in 93% of the patients. CONCLUSION: Paramedic weight estimates correlated well with measured weights.

Adult↗
PubMedDate not supplied

[Interactions between nucleic acid bases. New parameters of potential functions and energy minima].

The parameters of atom-atom potential functions suggested by one of the authors in 1979-1986 were slightly changed. The changes were performed to achieve a better agreement with experimental data of interaction energy values in global minima and hydrogen bond lengths. These changes resulted in better accord with experimental values of distances between the layers in DNA monomer crystals and between the base pairs in oligonucleotide duplexes. The refined potential functions were used to calculate the energy of interactions between nucleic acid bases in various mutual positions. The calculations revealed a few types of mutual base arrangements in minima of interaction energy for each pairwise base combination. A new type of minima was found, which correspond to a nearly perpendicular arrangement of base rings and the formation of the intermolecular hydrogen bond.

Algorithms↗
PubMedDate not supplied

[Interaction of caffeine with basic nucleic acids my a molecular mechanic method].

To understand some aspects of the biological action of caffeine (CAF), the interaction energies for various mutual positions of CAF and DNA bases or basepairs were calculated. Three types of mutual CAF-base (CAF-basepair) arrangements corresponding to the minima of interaction energy were revealed. One type of minima correspond to the stacking arrangement of molecules. This type is important for interactions of CAF with DNA monomers and single-stranded fragments. The other two types of minima correspond to the formation of intermolecular hydrogen bonds. Some of these minima may occur during the interaction of CAF with the double helix. One of these types corresponds to the nearly in-plane position of molecules. The other type of minima correspond to the nearly perpendicular arrangement of molecule planes. The minima of the last type are supposed to be the most important for the interaction of CAF with the DNA duplex, and interaction energies for this type of minima have the most negative values.

Caffeine↗
PubMedDate not supplied

[Possible configuration of dimers of Gua-Cyt bases. Computation by molecular mechanic models and density functional theory].

The energies of interactions between guanine and cytosine in various mutual positions were calculated by the methods of molecular mechanics with refined atom-atom potential functions and the quantum mechanics theory of density functional. Both methods indicate three types of mutual positions of bases in local energy minima. These types correspond to (1) nearly coplanar base positions with intermolecular hydrogen bond formation (base pairing); (2) arrangements of two bases in nearly parallel planes one above another (base stacking); and (3) nearly perpendicular positions of base planes. According to the calculations, the global energy minimum corresponds to the Watson-Crick base pair with three hydrogen bonds. A specific feature of the pair is a transition from many positions of type (2) to positions of type (1) without any energy barrier. This feature is revealed by both methods. Another special feature of this pair is a deviation, for most of mutual base positions, of the amine group atoms from the ring plane, the deviation being more pronounced for Gua. These features are important for understanding the conformational behavior of DNA fragments and the RNA structure.

Cytosine↗
PubMedDate not supplied

[Interactions of caffeine with DNA double helix fragments. Molecular mechanics simulation].

Calculations of the energy of interaction between the caffeine molecule and DNA double helix fragment of four complementary pairs have been performed by the molecular mechanics method. The calculations demonstrate the existence of energy minima corresponding to the caffeine molecule position in both wide and narrow grooves. Each of three proton acceptor atoms of caffeine is able to form hydrogen bond with each of three amino groups of DNA bases. The interactions of caffeine with both hydrogen bonded nucleotide and other nucleotides of the two strands contribute considerably to the total energy. The substantial contribution of interactions of caffeine with other than H-bonded nucleotides results in a rather close packing of atom groups in possible DNA-caffeine complexes. The mechanisms of influence of caffeine on interactions of DNA with other biologically active compounds are discussed.

Caffeine↗
PubMedDate not supplied

Thrombolytic therapy for acute myocardial infarction.

Numerous factors must be considered when determining the formulary status of thrombolytic agents for the treatment of acute myocardial infarction. Defined treatment options, predicted outcomes, and the economic consequences of this disorder continue to evolve from clinical trials. Pharmacists have a major role in delivering patient care, with responsibility for evaluating, procuring, and monitoring thrombolytic agents and drug therapy in general. By participating in the development and implementation of treatment guidelines, evaluating economic and therapeutic outcomes, providing timely optimal drug therapy, and educating health care providers and the public, they contribute significantly to the health care team.

Adjuvants, Pharmaceutic↗