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Biomedical subjects

E Giovannini

Publications and source records attributed to E Giovannini.

At least 91 records · Page 5Linked to original sources
PubMed1975

Different molecular weight of the plasmatic erythropoietin compared with the renal and splenic erythropoietic factors in the rabbit.

The authors have studied the molecular weight of the erythropoietic factors present in the blood, in the kidney and in the spleen of the rabbit, by means of ultrafiltration experiments performed by using selective-permeability membranes. The erythropoietic factor present in the blood circulation shows a molecular weight of between 10,000 and 30,000. The factors detectable in the microsomal fraction of normal kidney, in the mitochondrial fraction of anaemic kidney and in the mitochondrial fraction of normal spleen show molecular weights of between 50,000 and 100,000. These data confirm the hypothesis already formulated regarding the renal origin of the splenic erythropoietic factor and the different nature of the renal erythropoietic factor compared with the plasmatic erythropoietin.

Anemia↗
PubMedDate not supplied

S-fluorenylmethoxycarbonyl glutathione and diesters: inhibition of mammalian glyoxalase II.

Inhibitors having high specificity toward mammalian glyoxalase II, but not glyoxalase I, were sought as part of a program to study glyoxalase enzyme function in mammalian cells. The compound, S-fluorenylmethoxycarbonyl glutathione (FMOC-G), was synthesized and found to be a competitive inhibitor of purified calf liver glyoxalase II (Ki = 2.1 mumol/l). Inhibition constants (Ki values) for the other glyoxalase enzyme, glyoxalase I, and the glutathione-requiring enzyme, glutathione S-transferase, from other sources, were found to be 17 and 25 mumol/l, respectively. FMOC-G is a very poor inhibitor of glutathione reductase and glutathione peroxidase. Diesters (dimethyl, diethyl, diisopropyl) of FMCO-G were also synthesized, as proinhibitors, to improve transport of FMOC-G into mammalian tumor cells (rat adrenal pheochromocytoma, PC-12) in culture. The diesters were inhibitory to cell growth and variability; the most effective of these, diisopropyl FMOC-G, exhibited an [I]0.5 value of approximately 275 mumol/l. Diesters of FMOC-G may be useful in studies of the glyoxalase enzyme system in cultured mammalian cells.

Animals↗