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Biomedical subjects

E Ginter

Publications and source records attributed to E Ginter.

At least 19 recordsLinked to original sources

[Health status in Europe and projections to the year 2000. 1. The male population].

The life expectancy of men varies at present in the posttotalitarian central and eastern European countries round 67 years; on the territory of the former USSR it is cca 64 years, in western and northern Europe 72-75 years. Linear extrapolation of these data to 2000 indicates that at the beginning of the next century the life expectancy of men in western Europe will vary round 76 years, while in the posttotalitarian countries it will remain at the same low level. In western Europe there is a steady decline of early deaths of men due to cardiovascular diseases (ischaemic heart disease, cerebrovascular diseases), diseases of the respiratory, digestive, nervous, urogenital system. The mortality caused by neoplasms, however, remains at a constant level. This is the reason why after 2000 the structure of mortality will change substantially: the cause of premature death of every other man in western Europe will be neoplasms. In the posttotalitarian part of Europe since 1970 early deaths of men due to cardiovascular diseases, neoplasms (mainly lung cancer), diseases of the digestive and nervous system and cirrhosis of the liver are rising steadily. It is probable that in this part of Europe also after 2000 the main cause of death will be cardiovascular and neoplastic diseases which together will account for 60-70% of early deaths of men and for their short life expectancy.

Cause of Death

The effect of graded ascorbic acid intake on the activity of GSH-Px in the liver of female guinea pigs.

Differing antioxidant potentials created by graded ascorbic acid supplementation (1, 10, 100 mg per animal daily) evoked changes in the level of glutathione peroxidase activity and lipid peroxides in the liver of female guinea pigs. The group with the lowest ascorbic acid intake (1 mg) had the lowest activity of glutathione peroxidase and the highest level of lipid peroxides. The two other groups (10 and 100 mg) showed enhancement of glutathione peroxidase activity and decline in lipid peroxides. There was no difference between the groups with 10 and 100 mg ascorbic acid intake.

Animals

Cardiovascular risk factors in the former communist countries. Analysis of 40 European MONICA populations.

MONICA Project findings provide a unique opportunity to compare cardiovascular disease (CVD) risk factor levels in a large number of populations living in different political systems. 15 European communist populations had significantly higher age-standardized mortality for age groups 35-64 years from all causes, from CVD and stroke than 25 democratic MONICA populations. The prevalence of systolic and diastolic hypertension in males and females, prevalence of smoking in males and prevalence of overweight in females were significantly higher in communist MONICA populations. In these populations there was tendency to lower prevalence of hypercholesterolemia. In simple and multiple regression analysis hypertension and smoking prevalence had the highest relation with CVD mortality in men; hypertension and overweight prevalence had the highest relation with CVD mortality in women. The combination of 'classical' CVD risk factors could explain only part of differences observed. In communist Europe there was high consumption of spirits, low consumption of fruits and extremely low intake of citrus fruits. Instead of exaggerated anti-cholesterol propaganda emphasis should be given to the prevention of antioxidant deficiencies by the increase of fruit and vegetable consumption and to the decrease in salt, spirit and cigarette consumption in former communist countries.

Adult

[The role of antioxidants in the prevention of tumors].

The potential role of antioxidant vitamins (ascorbic acid, beta-carotene, alpha-tocopherol), minerals (selenium) and non-vitamin natural antioxidants (e.g. glutathione) in the prevention of cancer diseases is reviewed. Free oxygen radicals, especially the hydroxyl radical .OH modify nitrogen bases, split DNA, stimulate oncogene activators and probably in many other ways participate in carcinogenesis. In a great number of experimental and epidemiological studies a significant increase of cancer risk in laboratory animals and in humans with low antioxidant status was found. Significant protective effects of ascorbic acid, beta-carotene, alpha-tocopherol and selenium against the incidence of gastrointestinal and lung cancer were achieved in most, but not in all prospective and intervention studies. It is probable that extremely high premature cancer mortality in postcommunist countries of Central and East Europe is caused by high consumption of cigarettes, spirits and saturated fats, by pollution, and by very low consumption of the chief sources of natural antioxidants (fruits, vegetables). (Fig. 13, Ref. 29).

Animals

[Non-homogeneity of the average life span in Slovakia].

The life expectancy at birth of males in the Slovak Republic (SR) is one of the shortest in Europe. Exact explanation of this phenomenon is still unknown. The statistical significance of the relation between life expectancy and various factors (infant mortality rate, ethnical structure, educational level, religious belief, economic activity of inhabitants and environmental factors) was analysed by multifactorial methods in all 38 districts of Slovakia. A surprising paradox phenomenon was observed in the areas with the highest pollutant emissions, where the life expectancy was the longest. Infant mortality rate, ethnical structure and particularly the educational level of the districts were significantly correlated with life expectancy. The life expectancy was negatively influenced mostly by the proportion of elementary--educated males. We suppose the style of life presents the background of these relations, but there is a lack of statistically reliable data from the individual districts of SR for more profound analysis. It must be stressed that neither the statistically high significant correlation is evidence of the causal connection between observed parameters. (Tab. 1, Fig. 5, Ref. 16.)

Aged

Histopathological evidence of vitamin C protection against Cd-nephrotoxicity in guinea pigs.

The critical organ of chronic cadmium (Cd) exposure are kidneys. Long-term exposure leads to Cd accumulation in kidneys and causes renal tubular dysfunctions. Cd-induced renal changes are irreversible, so the primary prevention by different nutrients can play an important role. In this study, male guinea pigs which, like humans, do not synthetize ascorbic acid (AA) received low (2 mg/animal/day) or high (100 mg/animal/day) dosage of AA and cadmium (1 mg Cd/animal/day) in drinking water for 12 weeks. Characteristic finding in renal morphology of Cd-intoxicated guinea pigs was dilatation of interstitial veins with apparent paravenous lymphatic infiltrates. Histomorphological changes were more evident in Cd-intoxicated guinea pigs with low AA intake. High AA intake apparently reduced in Cd-intoxicated guinea pigs the extent of renal damage. Cadmium significantly increased serum creatinine and urea levels in the group of guinea pigs with low AA intake but not in guinea pigs receiving high concentration of AA. The results showed that AA can be effective in the protection of Cd-induced nephrotoxicity.

Animals

Interactions between hepatic ascorbic acid, cytochrome P-450 and lipids in female guinea pigs with different ascorbic acid intake.

Changes in serum and liver lipids, hepatic ascorbic acid (AA) and cytochrome P-450 were investigated in female guinea pigs divided into three groups with different AA intake in drinking water (10, 100 and 1000 mg AA per liter) for 10 weeks. Serum and liver total cholesterol significantly decreased in guinea pigs receiving 100 and 1000 mg AA per liter of drinking water when compared with guinea pigs with suboptimal AA intake (10 mg/l). Similarly, serum triglycerides were decreased in the groups with higher AA intake. Liver AA concentration increased significantly in accordance with rising AA doses. High AA intake (1000 mg/l) for 10 weeks resulted in significant increase of both cytochromes P-450 and cytochrome b5 and total haeme content in liver microsomes when compared to guinea pigs with suboptimal AA intake. A significant positive correlation between hepatic AA concentration and cytochrome P-450 content was observed. A close negative correlation between liver total cholesterol and cytochrome P-450 content in hepatic microsomes was also seen. Long-term suboptimal AA intake may unfavourably alter the blood and liver lipid profile as well as the capacity of hepatic drug metabolizing enzymes in both male and female guinea pigs.

Animals

Selenium concentration in plasma and erythrocytes in a healthy Slovak population.

To obtain reference values for plasma and erythrocyte selenium concentrations blood specimens were collected from healthy adults from the Bratislava region. In 174 people, 20 to 60 years old, a plasma Se concentration of 56.2 +/- 8.5 micrograms/L and in 133 persons an erythrocyte Se level of 95.3 +/- 16.3 micrograms/kg were found (mean +/- SD). Selenium levels showed a normal distribution in plasma and erythrocytes. No differences were found according to sex or age. These results indicate that the Slovak population has a low Se status, with values for plasma Se being among the lowest in Europe.

Adult

[The role of antioxidants in the prevention of cardiovascular diseases].

The potential role of natural antioxidants (vitamin C--ascorbic acid, vitamin E--tocopherols, carotenoids and selenium) in the prevention of cardiovascular diseases is reviewed. It is probable that free oxygen radicals and oxidatively modified particles of low-density lipoproteins (LDL) participate in the development of atherosclerotic lesions. A great number of experimental, cross-sectional, retrospective and prospective epidemiological studies found a substantial increase of the risk of ischemic heart disease and stroke in individuals and populations with low intake of antioxidants from diet. Extremely high cardiovascular mortality in Slovakia and other postcommunist countries could be only partially explained by "classical" risk factors (hypertension, hypercholesterolemia and smoking). In the communist European countries there was a high consumption of spirits, cigarettes and salt, polluted environment and low consumption of the chief source of antioxidants--fruits. In these countries emphasis should be given to the prevention of antioxidant deficiencies by the increase of fruit and vegetable consumption, and to the decrease in salt, spirit, cigarettes and saturated fat consumption.

Antioxidants

Erythrocyte membrane fluidity and tissue lipid peroxides in female guinea-pigs on graded vitamin C intake.

The effect of low (1 mg/animal/day), medium (10 mg/animal/day) and high (100 mg/animal/day) intake of ascorbic acid on tissue lipid peroxidation (LPO) and the physical state of erythrocyte membranes was investigated in female guinea-pigs fed a vitamin E low diet. Animals were killed after 9-11 weeks and the blood, liver, lungs, kidneys and adrenals were analysed. The LPO was estimated by the determination of malondialdehyde with HPLC. The physical state of erythrocyte membranes was determined spectrofluorometrically and expressed as fluorescence polarization of membrane lipid specific probe 1,6-diphenyl-1,3,5-hexatriene. The LPO concentrations in the liver and adrenals of the group on a low vitamin C intake were significantly increased. A significant non-linear negative correlation between C vitamin levels and LPO concentrations was found in these tissues. The fluidity of erythrocyte membranes as a measure of their structural state was significantly lower in the group with a low intake of C vitamin. It is probable that the water-soluble antioxidants, such as vitamin C, act in the plasma as primary defense against oxidative stress if the radicals are formed initially in the aqueous phase of whole blood.

Adrenal Glands

Serum copper, zinc and copper/zinc ratio in males: influence of aging.

Apparently healthy free-living (non-hospitalized) men aged 8-89 years (n = 408) were studied to determine the effect of age on serum copper and zinc concentrations and the copper/zinc ratio. Mean values +/- S.D. for age, serum copper and zinc levels and the copper/zinc ratio were as follow: 41.9 +/- 22.9 years, 1.15 +/- 0.17 micrograms/ml, 0.93 +/- 0.14 micrograms/ml and 1.25 +/- 0.19, respectively. In the elderly subjects above 75 years, a marked increase in serum copper concentrations and the copper/zinc ratio as well as a decrease in serum zinc concentrations were observed. Serum copper concentrations and the copper/zinc ratio correlated positively with age (p < 0.0001).

Adolescent

[Determination of ascorbic acid in blood serum using high-performance liquid chromatography and its correlation with spectrophotometric (colorimetric) determination].

Insufficient supply of the populations of the Czech and Slovak Republics with vitamin C is connected with the occurrence of some diseases due to modern civilization. It is therefore necessary to obtain reliable data about the supply of the population with this most important antioxidant of blood plasma. In order to establish a simple, rapid and at the same time specific determination of vitamin C in serum, the present authors have introduced, on the basis of foreign data, the HPLC method of determination of ascorbic acid on reverse phases with UV-detection (245 nm), using 3.7 mM KH2PO4 (pH = 4) as the mobile phase. Very good separation of ascorbic acid from serum (Fig. 1) was obtained. The detection limit is 3.25 pmol of ascorbic acid. Parallelly with the chromatographic determinations, ascorbic acid was determined in 235 volunteers also by the spectrophotometric (colorimetric) method employing 2,4-dinitrophenylhydrazine. Both methods correlate very well (Fig. 2): correlation coefficient, r = 0.93, the level of probability p < 0.000001. The yield (Table 1) is 98.6% and 97.6% for HPLC, or 105.4% and 111.4% for the colorimetric method. The reproducibility of both methods gives coefficients of variation of 3.6% and 2.9%. The described HPLC method is sufficiently precise, specific and suitable for the determination of the levels of ascorbic acid in serum.

Adult

Effect of the oyster fungus on glycaemia and cholesterolaemia in rats with insulin-dependent diabetes.

We have investigated the effect of a diet containing of 4% oyster fungus (Pleurotus ostreatus) and 0.1% cholesterol on glycaemia and hyperlipoproteinaemia in rats with insulin-dependent diabetes (streptozotocin 45 mg/kg). After two months, the rats with diabetes kept on the oyster fungus diet, had a significantly lower basal and postprandial glycaemia, the insulinaemia remained unchanged. The cholesterol concentration was decreased by more than 40%, the lipoprotein profile was upgraded by the decrease of the cholesterol in both the low density and very low density lipoproteins. The oyster fungus decreased the cholesterol accumulation in the liver and had no significant effects on the levels of serum and liver triacylglycerols.

Animals

Response of hepatic drug-metabolizing enzymes to immobilization stress in rats of various ages.

The effects of acute two-hour immobilization stress in hepatic microsomal drug-metabolizing enzyme activities, liver and blood serum zinc and copper levels, content of vitamin C in adrenals, were studied in rats of three age categories (4, 12, 24 months old). Immobilization stress resulted in marked reduction in the concentrations of vitamin C in adrenals of all age categories. In stressed animals, the activities of microsomal aniline hydroxylase, p-nitroanisol 0-demethylase, and the hepatic concentrations of microsomal protein were significantly increased in young 4-month-old rats, especially. In these rats increased levels of zinc in blood serum and copper in the liver, were seen. The levels of zinc and copper in older rats after immobilization stress were apparently unchanged. Age-dependent decrease in the activities of hepatic microsomal monooxygenases and adrenal vitamin C content in control rats were observed.

Adrenal Glands

Effect of the oyster fungus on glycaemia and cholesterolaemia in rats with insulin-dependent diabetes.

We have investigated the effect of a diet containing of 4% oyster fungus (Pleurotus ostreatus) and 0.1% cholesterol on glycaemia and hyperlipoproteinaemia in rats with insulin-dependent diabetes (streptozotocin 45 mg/kg). After two months, the rats with diabetes kept on the oyster fungus diet, had a significantly lower basal and postprandial glycaemia, the insulinaemia remained unchanged. The cholesterol concentration was decreased by more than 40%, the lipoprotein profile was upgraded by the decrease of the cholesterol in both the low density and very low density lipoproteins. The oyster fungus decreased the cholesterol accumulation in the liver and had no significant effects on the levels of serum and liver triacylglycerols.

Animal Nutritional Physiological Phenomena

The effect of ascorbic acid on cadmium accumulation in guinea pig tissues.

Accumulation of cadmium in organs of guinea pigs after subchronic oral cadmium treatment (1 mg Cd/animal/24 h) was in the following order: kidneys > liver > heart > testes > brain. The preventive effects of high doses of ascorbic acid (AA) against cadmium deposition were more pronounced in the testes, heart and brain, and in the kidney only after short-term cadmium treatment. Ascorbic acid had no protective effect on cadmium accumulation in the liver.

Analysis of Variance

The influence of ascorbic acid on lipid peroxidation in guinea pigs intoxicated with cadmium.

Cadmium (as CdCl2) administered at a dose of 1 mg Cd/animal/day in drinking water to guinea pigs with a low intake of ascorbic acid (2 mg/animal/day) increased lipid peroxidation (LPO) in the kidney, liver and serum after 5 wk of treatment. A high intake of ascorbic acid (100 mg/animal/day) decreased LPO (determined from malondialdehyde levels) in the kidney, liver and serum of Cd-treated guinea pigs. Administration of Cd to guinea pigs with a low intake of ascorbic acid significantly increased LPO in the kidney at 5 wk, in comparison with controls given low levels of ascorbic acid but no Cd. However, at 12 wk, LPO in the kidney of Cd-treated guinea pigs was significantly lower than in the controls. These results indicate that the level of lipid peroxides in the kidney of guinea pigs with a low intake of ascorbic acid depends on the amount of accumulated cadmium.

Administration, Oral