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Biomedical subjects

E G Kolokol'chikova

Publications and source records attributed to E G Kolokol'chikova.

At least 19 recordsLinked to original sources
PubMed2006

Radioautographic study of palatine tonsillar cells.

Proliferative and functional (protein producing) activities of palatine tonsillar cells were studied by light radioautography (semithin sections). 3H-Thymidine incorporation demonstrated the dynamics of renewal of palatine tonsillar cell populations in various pathological states. 3H-Uridine labeling objectively evaluated functional activity of different cells and more accurately showed distribution of destructive processes in the organ.

Adult↗
PubMed2006

[Morphological radioautographic study of the palatine tonsils in chronic tonsillitis and healthy subjects].

A pilot morphological (radioautographic) study of healthy and affected palatine tonsils (in hypertrophy and toxic-allergic form of chronic tonsillitis) in children and adults has shown that intensity of biosynthetic processes (protein-synthetic -- RNA synthesis and proliferative activity -- DNA synthesis) in the cells of palatine tonsils and structural changes of their tissues depend on characteristics of pathological process. Microbial penetration into the tonsils with formation of an active focus of chronic infection is traced.

Adolescent↗
PubMed2005

Proliferative activity of adipocytes in adipose tissue tumors.

Electron-autoradiographic study of normal and tumor-transformed adipose tissue (common lipoma and destructive lipoma, i.e. infiltrating and degrading lipoma) showed the capacity of adipose tissue cells in lipomas, especially in destructive lipomas, to proliferation and differentiation. In vivo synthesis of DNA in mature adipocytes not observed previously is described. The role of microvascular wall cells as mesenchymal multipotent precursors in the formation of the adipose tissue is discussed. The involvement of the bone marrow mesenchymal stem cell in this process cannot be ruled out.

Adipocytes↗
PubMed2004

Autoradiographic study of parasympathetic paragangliomas (chemodectomas) of the neck.

Study of the morphofunctional characteristics of chemodectomas of the neck by photoautoradiography (semithin sections) showed the absence of protein-producing and proliferative activity in light and dark tumor cells against the background of strained biosynthetic processes in stromal cells. Invasion of capillary walls by tumor cells with their release into the capillary lumen is a prerequisite for metastases of these tumors.

Adult↗
PubMed2002

[Immune status in diabetic patients with pyo-necrotic lesions of lower extremities].

Long-term and severe pyonecrotic processes in diabetic patients testify to severe disorders of immune system in this disease. High titer of antibodies to tested autostrain demonstrated its etiologic role in infectious process. The study group consisted of 29 patients (with diabetic pyonecrotic foot lesions), control group--17 patients with burns of III a, b--IV stage affecting from 20 to 60% of body surface. In diabetic patients antibodies titer to the most encountered infectious agents Staphilococcus aureus and Pseudomonas aeroginosa was lower than in burn patients with immunity deficiency. Decrease of antibodies titer in diabetic patients testifies to high insufficiency of B-immunity.

Adolescent↗
PubMed2002

[Efficacy of clinical-serologic diagnosis of wound infection in burned patients].

Titer of antibodies to microorganisms obtained from wound and blood of 46 patients with burns of IIIAB--IV degree was studied. The majority of the burned patients have ability to synthesize antibodies. Agglutination of the bacteria by antibodies promoted location of infectious agent in primary focus and impeded bacteria to spread into blood and generalization of process. Not all microorganisms of wound provoke antibodies synthesis, i.e. wound infection. Therefore bacteriologic study must be supplemented by serum diagnosis. Detection of bacteria in the blood without serologic data do not permit to consider these bacteria as etiologic factor of wound infection.

Adolescent↗
PubMed2001

Morphological changes in burn wounds after transplantation of allogenic fibroblasts.

Cultured allogenic fibroblasts labeled with fluorescent acridine orange and transplanted onto burn wound virtually completely disappear from the wound surface within 2 days (are destroyed). Study of wound morphology by light autoradiography and quantitative analysis showed a stimulating effect of transplanted fibroblasts on wound healing. This effect, irrespective of the initial state of the wound, is always directed at accelerated formation of connective tissue similar to the derma and capable of epithelialization.

Acridine Orange↗
PubMed2000

[Morphological examinations of infected burn wounds].

Electron microscopical and electron radioautographic methods have been used for morphological examination. This allowed to raise sensitivity of the tests facilitating detection of living solitary bacterial cells (by intensive mark above them). Radioautography showed functional status of wound bacteria (the intensity of the metabolism and reproduction). It was determined that in living tissues, not damaged by the burn and unchanged morphologically bacteria do not penetrate. Especially massive accumulations of the microorganisms are found in necrotic tissues, under the scab, at the external border of demarcation protuberance, and they represent colonization of the wound. When modern methods of treatment and early necrectomy are used, morphological examination usually does not reveal pathogenic microorganisms. Only inflammatory reaction of the wound tissues could be seen: oedema, hyperemia, hemorrhages, cellular infiltrates. The development of granulation tissue indicated cessation of wound infection. Especially favourable feature, demonstrating healing of the wound, was appearance and reproduction of young cells of fat tissue (indusion of labelled thymidine). The results of the study have confirmed the advantage of new electronic methods for microscopy in infected burn wounds.

Autoradiography↗
PubMed1999

[Substantiation and varieties of complex surgical treatment tactics for pyo-necrotic forms of diabetic foot].

For the period from 1996 to 1998 in the Division of wounds and wound infection of A.V. Vishnevsky Institute of Surgery 92 patients with pyonecrotic forms of "diabetic foot" underwent thorough examination and treatment. The patients were divided into groups by the form of "diabetic foot": with pyonecrotic forms of "diabetic foot" without critical ischemia (group 1) and with it (group 2). In 18 patients of both groups the data of electron autoradiography were used to reveal peculiarities of the wound process. Group 1 patients had at admittance a large number of neutrophiles in various stages of destruction in biopsies of the wound. In patients of group 2 a great majority of the vessels in biopsies of the wound were in different stages of destruction with lost connections between their separate cells or some of their part absent. Separate cells (endotheliocytes and pericytes) which make up the walls of destroying vessels, were synthesizing RNA and were functionally active. In both groups, the studied parts of the wound before plastic repair of its defect usually represented as well developed granulation tissues with a number of microvessels and cells. Intensive synthesis of PNA in the cells of microvascular wall evidenced of their high functional activity, and the synthesis of DNA in them showed their ability for proliferation, i.g.--for growth. Thus, microangiopathy was reversible, and the solution of the problem of critical ischemia should be considered in the light of macroangiopathy. Thus, in patients of group 1 the cause of pyonecrotic damage consists in infection process, while in patients of group 2--in combination of infection with ischemia of the extremity. In both groups pyonecrotic disease of the extremity ruses at the background of severe disturbances of cellular immunity.

Diabetic Foot↗
PubMed1998

[Thrombin--a regulator of reparative processes in wound healing].

Thrombin, binding to receptors of the protease activated receptor (PAR) family, is involved in wound healing by inducing the reparation processes and regulating the activity of mast cells, which secrete mediators of inflammation. Using thrombin receptor agonist peptide (TRAP-6) for the activation of rat mast cells, effect of several receptors, including PAR-1, on mast cells was demonstrated. It was shown that TRAP increases the concentration of Ca2+ in the cytoplasm of mast cells and regulates cell degranulation, while releasing nitrogen oxide. Thrombin encapsulated in poly(N-vinyl caprolactam)-calcium alginate (PVCL-Ca-Alg) hydrogel films promotes wound healing in rats as demonstrated by the acceleration of fibroblast proliferation and neovascularization.

Animals↗