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Biomedical subjects

E G Dediukhina

Publications and source records attributed to E G Dediukhina.

At least 19 recordsLinked to original sources
PubMed1984

[Microorganisms as potential producers of essential fatty acids].

The data on the importance of essential fatty acids in a balanced diet have been considered. The ways of essential fatty acid synthesis in microorganisms and their metabolism in animal tissues are being discussed. The criteria for microorganisms selection--producers of food lipids have been proposed.

Animal Nutritional Physiological Phenomena↗
PubMed1982

[Effect of the growth rate on the biomass composition of Candida valida].

The effect of growth rate on the biomass composition was studied with a thermotolerant strain of the yeast Candida valida grown in the chemostat at limitation of the growth by ethanol under the conditions of various temperature regimes. The temperature and the growth rate hardly influenced the content of protein and its amino acid composition. An increase in the specific growth rate of the cells caused a rise in the pool of free intracellular amino acids, in particular, glutamic acid. The degree of lipid unsaturation increased with a rise in the growth rate of the yeast as a result of intensification of oleic acid oxidative dehydration. As it had been shown earlier, the content of nucleic acids increased with a rise in the specific growth rate of the yeast.

Amino Acids↗
PubMedDate not supplied

[Effect of conditions od Trichosporon pullulans culturing on the synthesis of immunomodulating glycoprotein].

An extracellular glycoprotein (GP) exhibiting immunomodulating activity produced by the yeast Trichosporon pullulans grown in a defined ethanol-containing medium differed substantially in its composition from that of the yeast cell walls: therefore, it cannot be considered a structural component of the cell walls. In batch culture, the greatest GP production (40 mg/l) occurred in the exponential phase of the yeast growth. Under continuous cultivation, in both chemostat and pH-auxostat regimes, the specific rate of GP synthesis (qGP) increased with the increasing specific growth rate (mu) and reached 1.55 mg/(g h) at mumax. Under limitation of the yeast growth by zinc qGP was three times lower than under nitrogen or iron limitation. The rate of GP production depended inversely on the oxygen concentration.

Adjuvants, Immunologic↗
PubMedDate not supplied

[Growth-coupled lipid synthesis in Mortierella alpina LPM 301, a producer of arachidonic acid].

Mortierella alpina LPM 301, a producer of arachidonic acid (ARA), was found to possess a unique property of intense lipid synthesis in the period of active mycelium growth. Under batch cultivation of this strain in glucose-containing media with potassium nitrate or urea, the bulk of lipids (28-35% of dry biomass) was produced at the end of the exponential growth phase and remained almost unaltered in the stationary phase. The ARA content of lipids comprised 42-50% at the beginning of the stationary phase and increased continuously after glucose depletion in the medium due to the turnover of intracellular fatty acids; by the end of fermentation (189-210 h), the amount of ARA reached 46-60% of the total fatty acids (16-19% of dry mycelium). Plausible regulatory mechanisms of the growth-coupled lipid synthesis in microorganisms are discussed.

Arachidonic Acid↗
PubMedDate not supplied

[Changes in the composition of intracellular lipids and hydrocarbons in Candida tropicalis in the absence of growth].

The fatty acid composition of lipids and the composition of hydrocarbons were studied in Candida tropicalis cultivated on a medium with propionic acid and incubated in the conditions of starvation and on a medium containing glucose-1-6-14C but no nitrogen. Intracellular fatty acids with an odd number of carbon atoms were found to be easily metabolized by yeast cells. The content and composition of intracellular hydrocarbons were very labile and underwent intensive transformation caused by changes in the metabolism of the yeast cell.

Candida↗
PubMedDate not supplied

[Bacterial degradation of EDTA].

Degradation of EDTA (ethylenediaminetetraacetic acid) or metal-EDTA complexes by cell suspensions of the bacterial strain DSM 9103 was studied. The activity of EDTA degradation was the highest in the phase of active cell growth and decreased considerably in the stationary phase, after substrate depletion in the medium. Exponential-phase cells were incubated in HEPES buffer (pH 7.0) with 1 mM of uncomplexed EDTA or EDTA complexes with Mg2+, Ca2+, Mn2+, Pb2+, Co2+, Cd2+, Zn2+, Cu2+, or Fe3+. The metal-EDTA complexes (Me-EDTA) studied could be divided into three groups according to their degradability. EDTA complexes with stability constants K below 10(16) (lg K < 16), such as Mg-EDTA, Ca-EDTA, and Mn-EDTA, as well as uncomplexed EDTA, were degraded by the cell suspensions at a constant rate to completion within 5-10 h of incubation. Me-EDTA complexes with lg K above 16 (Zn-EDTA, Co-EDTA, Pb-EDTA, and Cu-EDTA) were not completely degraded during a 24-hour incubation, which was possibly due to the toxic effect of the metal ions released. No degradation of Cd-EDTA or Fe(III)-EDTA by cell suspensions of strain DSM 9103 was observed under the conditions studied.

Biodegradation, Environmental↗
PubMedDate not supplied

[Bacterial strain characterizing by EDTA requirement].

A novel strain of bacteria (LPM-4) was isolated that is characterized by a unique EDTA requirement for cell growth. Suspensions of washed cells of strain LPM-4 degrated EDTA complexes with Ba2+, Mg 2+, Ca2+, and Mn2+ at constant rates (0.310-0.486 mmol EDTA/(g h)) and Zn-EDTA at an initial rate of 0.137 +/- 0.016 mmol EDTA/(g h). The temperature optima for cell growth and EDTA degradation were determined under pH-auxostat cultivation. As compared with the known EDTA-degrating bacteria, strain LPM-4 exhibited a higher specific growth rate (0.095 h(-1)) and lower mass cell yield (0.219 g cells/g EDTA) that is promising for its practical applications for EDTA removal in wastewater treatment plants.

Culture Media↗