Biomedical subjects

E G Bulanova

Publications and source records attributed to E G Bulanova.

3 recordsLinked to original sources

PubMed1995

Bioluminescent assay for human lymphocyte blast transformation.

One of the basic tests of in vitro evaluation of immune cell functional activity is a proliferative response of lymphocytes on the action of external stimuli such as mitogenic lectines, antigens, etc. We compared two methods used to assess the lymphocyte functional status. (1) [3H]thymidine incorporation and (2) bioluminescence for determination of intracellular ATP in blast cells. Comparison has been done for healthy donors and patients with proven low immunological status. The proposed bioluminescent method for evaluation of the proliferative response was shown to be sensitive enough for diagnostic purposes. This method allows one to process a large number of samples at the same time and correlates highly with the radionuclide test use hazardous radioactive materials.

Adenosine Triphosphate↗

PubMedDate not supplied

[Protein phosphatases: structure and function].

The process of protein and enzyme systems phosphorylation is necessary for cell growth, differentiation and preparation for division and mitosis. The conformation changes of protein as a result of phosphorylation lead to increased enzyme activity and enhanced affinity to substrates. A large group of enzymes--protein kinases--is responsible for phosphorylation process in cell, which are divided into tyrosine- and serine-threonine-kinases depending on their ability to phosphorylate appropriate amino acid residues. In this review has been considered the functional importance and structure of protein phosphatases--enzymes, which are functional antagonists of protein kinases.

Phosphoprotein Phosphatases↗

PubMedDate not supplied

[Tyrosine protein kinase: role in the process of activating lymphocytes].

Some types of receptor and intracellular protein-tyrosine kinases are reviewed. These enzyme systems play an important role in activation of T- and B-lymphocytes and their precursors. The relationship is shown between the two main ways of lymphocyte activation: the phosphatidylinositol metabolic pathway, induced by protein kinase C, and the protein-tyrosine kinase pathway of intracellular protein and enzyme phosphorylation.

B-Lymphocytes↗