[Histochemical study of the mucins in the mucosa adjacent to colo-rectal carcinomas associated with single or multiple adenomatous polyps].
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Adenocarcinoma↗
Biomedical subjects
E Fulcheri
Publications and source records attributed to E Fulcheri.
Immunohistochemistry: a new outlook in histopaleopathology.
To date, rehydration techniques on mummified tissues as well as the methods for histological specimen preparation with standard basic stains and with histochemical techniques are commonly known and well established. The application of immunohistochemical techniques is a further development in the study of mummified tissues. Similarly to what is done on fresh and fixed tissues, reaction sensitivity and specificity must be guaranteed. While reaction sensitivity depends on serum quality and the detection method employed, specificity is guaranteed by serum quality but also by method standardisation. Method standardisation is the most serious problem in the study of mummified tissues. Mummified tissues have different antigen preservation characteristics, varying from subject to subject, and even within the same subject, depending on the topographic region and the site. These changes are due to different mummification methods and to subsequent environmental conditions in which the mummy was preserved. The Streptavidin-Biotin-Peroxidase Complex (SABC) technique guarantees excellent detection sensitivity. However, the method accuracy and reliability can be ensured only through a rigorous case by case examination with internal controls on structures in which the antigens we are looking for are surely expressed. Today, with these artifices (technique optimisation), cell differentiation phenotype markers can be detected which are useful in paleopathological studies and in the diagnosis of morphologically doubtful lesions.
Antigens, Differentiation↗
Human papillomavirus in cervical adenocarcinoma. An in situ hybridization study.
Twenty cervical adenocarcinomas (CACs) in women aged 22 to 71 were investigated by in situ hybridization (ISH) with 6, 11, 16, 18, 31, 35 and 51 HPV biotinylated probes. Two cases, one adenocarcinoma and one adenosquamous carcinoma (in women aged 28 and 40 respectively) showed focal nuclear positivity to 16 HPV Probe in some neoplastic glands. We used ISH, rather than other hybridization techniques, in order to exclude a positivity to viral DNA, due to adjacent squamous epithelium, either normal or metaplastic, and in squamous foci within adenosquamous tumors. Reviewing the literature, we found 33 out of 98 CACs positive to viral DNA by ISH (33.6%). In spite of the differences found from author to author, a relationship between adenocarcinomas of the uterine cervix and HPV infection seems to be possible, as was demonstrated for CIN and invasive cervical carcinomas. These data could explain why the incidence of this neoplasia has tended to increase over the last few years, mainly in younger patients.
Adenocarcinoma↗