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Biomedical subjects

E Elliott

Publications and source records attributed to E Elliott.

At least 55 records · Page 3Linked to original sources
PubMed1990

Analysis of colloidal gold probes by isoelectric focusing in agarose gels.

Colloidal gold particles of different size (3-20 nm in diameter) were prepared by tannic acid-citrate and citrate reduction methods. From these colloids, different probes were prepared using sheep anti-rabbit antiserum, sheep anti-rabbit IgG, bovine serum albumin, polyethylene glycol, and protein A as the primary stabilizers and polyethylene glycol and/or bovine serum albumin as secondary and tertiary stabilizers, in different combinations. The probes were analyzed by isoelectric focusing in agarose gels, which allow the migration of particles in the size range 3-20 nm. (P. Sewer and S. J. Hayes, 1986, Anal. Biochem. 158, 72-78). Isoelectric focusing revealed that the surface charge of colloidal gold probes is dependent upon the size of the gold particle, the reduction method used, the primary ligand, and the pH at which this is adsorbed, as well as upon the secondary and tertiary stabilizers used. It is proposed that such differences in surface charge may underlie the different results which may sometimes be observed in colloidal gold labeling, especially when novel ligands are used.

Colloids↗
PubMed1989

Genetics and expression of the Q6 and Q8 genes. An LTR-like sequence in the 3' untranslated region.

A unique 2.2-kb mRNA is transcribed from the Q6 and Q8 genes of the mouse major histocompatibility complex. RNase protection experiments and DNA sequence analysis have mapped the 3' terminus to a site located 1110 bp downstream from exon 8. Comparison of the 3' sequence of Q8 to that of Q7 revealed that the two genes diverge from one another at a point located 200 bp into the untranslated domain. This finding explains the increased size of the transcript. RNase protection experiments involving twelve different strains of mice have revealed few sequence polymorphisms. A tissue distribution of the 2.2-kb transcript in B6 mice revealed that the highest quantities of message were present in the spleen with decreasingly lower amounts in the thymus, liver, kidney, testis, and brain. The H-2r haplotype appeared novel; it is phenotypically Qa-2-, yet expressed a 2.2-kb transcript which hybridizes to a Q8 probe. A comparison of a Qa-2hi expressor, B6, and a Qa-2low expressor, BALB/cFla, has established that these quantitative cell surface differences are reflected in mRNA differences. A homology search of the Genbank database has revealed that the 3' portion of Q8 contains extensive homology to a retrovirus-like long terminal repeat sequence that is characteristic of an embryonic-specific transposon (ETn).

Alleles↗
PubMed1988

Water, electrolyte, glucose, and glycine absorption in rat small intestinal transplants.

Water, electrolyte, glucose, and glycine absorption were studied in vivo in successful rat small intestinal transplants. Isolated bowel loops were transplanted from F1 hybrids into parental strain Lewis rats. A 7-day course of cyclosporin A was given for immunosuppression. Absorption was studied using a steady-state perfusion technique at either 9 or 21 days after transplantation. Histologic examination showed there was villus shortening with time but no evidence of rejection. When perfused with isotonic saline, both allografts and controls secreted water. However, allografts and denervated controls secreted chloride, whereas innervated controls absorbed chloride (p less than 0.05). There was a marked reduction in water and sodium absorption from 30 mM glucose-saline in transplanted loops and denervated controls, whereas glucose absorption was relatively preserved in these groups at 9 days (p less than 0.01). These changes could not be accounted for by rejection or ischemia. These studies demonstrate that denervation may be a major limiting factor in intestinal transplantation.

Animals↗
PubMed1987

Novel mRNA species from a Q-subregion class-I gene of the murine major histocompatibility complex.

A novel mRNA species is specified by the Q6/Q8 gene, a member of the Qa-2-determining family of class-I genes of the murine major histocompatibility complex. It is longer than all previously described class-I transcripts due to an extension at the 3' end of the mRNA past the predicted polyadenylation site. This mRNA was detected by Northern-blot analyses with single or low-copy-number probes to the 3' end of the Q6/Q8 gene. RNase protection experiments have confirmed this finding. The appearance of this mRNA in different strains of mice correlates with the synthesis of the Qa-2 molecule.

Animals↗
PubMed1985

Immediate free vascularized joint transfer from second toe to index finger proximal interphalangeal joint: a case report.

The results of treatment of severe injuries to the proximal interphalangeal joint are unsatisfactory. The methods of joint reconstruction are discussed, including fusion, implant arthroplasty, perichondrial grafting and vascularized joint transfer. A patient is presented with a severe crush injury to the dorsum of the index finger with loss of skin and extensor tendon and proximal interphalangeal joint disruption. Immediate reconstruction of the finger is described using a composite free flap of skin, extensor tendon and proximal interphalangeal joint from the second toe. Follow-up at two years is described, demonstrating proximal interphalangeal motion and finger function.

Adult↗
PubMed1981

Trabecular cell carcinoma--report of a case.

Trabecular cell carcinoma is a little known tumor with potentially aggressive behavior. Once a diagnosis has been established, aggressive surgical treatment should follow, with wide excision. Other modalities to be considered are radiation therapy and possible chemotherapy, for which no precedent exists. The tumor resembles metastatic anaplastic carcinomas, and familiary with it is important in the evaluation of cutaneous malignancies. A 55-year-old patient with trabecular cell carcinoma is presented.

Adenocarcinoma↗
PubMed1981

Chest wall shape during forced expiratory maneuvers.

Abdominothoracic shape during the forced vital capacity was studied in 10 normal subjects using magnetometers to monitor anteroposterior diameters at the level of the manubrium, xiphoid, and epigastrium, lateral rib cage diameter at the xiphoid level, and vertical motion of the rib cage. Thoracic cross-sectional area change at the xiphoid level was found to lag lung volume change, due to an early paradoxical increase (or lack of change), of lower anteroposterior rib cage diameter. To the extent that the resulting rib cage deformation can influence the pleural pressure gradient, the observed shape changes provide a potential mechanism for early preferential emptying of the upper lobes and later more homogeneous emptying in forced, compared to slow, vital capacity maneuvers. Comparisons of shape changes during Valsalva and abdominal expiratory ("expulsive") maneuvers suggest that lower rib cage deformation may not simply be due to the action of rib cage muscles affecting predominantly the lateral rib cage but rather the results of diaphragmatic activity and the influence of abdominal shape on the lower rib cage.

Adult↗
PubMed1981

Pattern of diaphragmatic activity during forced expiratory vital capacity.

We measured transdiaphragmatic pressure (Pdi) during forced expiratory vital capacity (FVC) maneuvers in 13 normal subjects and electromyographic activity of the diaphragm (edi) in 8 of these subjects. In all subjects, Pdi increased at the initiation of the FVC. In most, this increase lasted 30--50 ms and reached levels well above the Pdi observed at total lung capacity (TLC). After the initial transient increase, approximately half of the subjects demonstrated a substantial fall in Pdi to values near the relaxation level in the mid-vital capacity (VC) volume range, while half showed a second large increase in Pdi in this volume range. Seven of eight subjects tested showed a rapid decrease in Edi at the onset of the FVC, reaching a minimum in 30--50 ms. After this initial transient decrease, Edi increased in six subjects in the mid-VC volume range, in association with secondary rises in Pdi. In two subjects, Edi remained low throughout the remainder of the FVC, and Pdi in the mid VC range was generally lower. These results are consistent with the conclusion that the diaphragm is neither electromyographically silent nor mechanically unimportant during the FVC. Changes in abdominothoracic configuration, superimposed upon "antagonistic" activity of the diaphragm, result in substantial reductions in pleural (esophageal) pressure that may influence regional lung emptying during the FVC.

Abdominal Muscles↗
PubMed1980

Family planning.

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Contraception↗
PubMed1976

Ketamine anaesthesia for medical procedures in children.

Ketamine hydrochloride 2 mg/kg, together with atropine 0.2 mg, has been given intravenously on 100 occasions on a general paediatric ward. No serious side effects occurred. Dreams followed in 4 children but did not reduce acceptability of the drug. In our hands it has greatly reduced the pain and distress of children undergoing many routine medical procedures, particularly the dread which builds up when these have to be repeated in the same child. It has also produced close to ideal conditions for the operator, and probably increased his efficiency by reducing the emotional strain which occurs when doing painful things to a frightened patient.

Adolescent↗