A technique for initiating cultures of epithelial (urothelial) cells from mammalian urinary bladder has been described. Urothelial cells obtained by this method have been used to support replication of viruses and as controls for immunological, biochemical, chromosome, and electron microscopy studies. Both light and electron microscopic studies of cultured cells suggest that they are epithelial and not a mixed culture.

Cells, Cultured↗

PubMedDate not supplied

Vinblastine, intermittent bleomycin, and single-dose cis-dichlorodiammineplatinum(II) in the management of stge III testicular cancer.

A program for treating advanced testicular cancer with vinblastine, bleomycin, and a single dose of cis-dichlorodiammineplatinum(II) was used in an attempt to reduce the toxicity of a 5-day regimen while maintaining a high response rate. In ten previously untreated and six previously treated patients, the complete remission rate was 25%. Toxic reactions were frequent, and there was one drug-related death. This single-dose cis-dichlorodiammineplatinum(II) program is ineffective in the treatment of testicular cancer. Other means for reducing toxicity will have to be considered.

Adolescent↗

PubMedDate not supplied

Prognostic factors in cancer of the testis: an approach to identification.

The clinical experience at the University of Minnesota Hospitals between 1962 and 1977 was reviewed in the hope of defining the biologic features of testicular cancer, knowledge of which is essential to determine the therapeutic needs of each patient. This collaborative effort between clinicians and statisticians has attempted to define important variables in the assessment of risk factors that determine the choice of treatment for testicular cancer. A preliminary analysis of data on the first 78 patients tabulated was performed to test and plan an approach to the final analysis of a larger group of 282 patients. One prognostic factor, staging, is discussed in detail to illustrate the purpose of this exploratory testing. Retrospective analysis, according to stage, of patients who were referred to this cancer center revealed a poorer prognosis than should be anticipated. In reporting the clinical experience of an individual institution, problems of bias are introduced with respect to the nature of the types of tumors studied.

Actuarial Analysis↗