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Biomedical subjects

E E Anderson

Publications and source records attributed to E E Anderson.

At least 37 records · Page 2Linked to original sources
PubMed1987

The ability to convert the 4 S glucocorticoid receptor to the 7-8 S form is dependent on both RNA and protein factors.

The DEAE-cellulose-purified 4 S form of the rat liver glucocorticoid receptor can associate with cytosolic factors, as evidenced by an alteration of the sedimentation value of the 7-8 S form. On the basis of sedimentation profile, this form is indistinguishable from the activated, low-salt 7-8 S form isolated from rat liver cytosol. In addition, both the endogenous and reconstituted 7-8 S receptor can bind DNA as the 7-8 S form. In keeping with our reports that the endogenous form of the 7-8 S receptor is sensitive to RNAase digestion, treatment of the cytosol with RNAase prior to mixing with the 4 S receptor prevents the formation of the 7-8 S material. Moreover, warming the cytosol to 50 degrees C prior to mixing with the 4 S receptor also eliminates the ability to form the heavier material. Since RNA is heat-stable, this suggests that other factors may be involved. Treatment of the cytosol with N-ethylmaleimide, a reagent reported to be specific for sulfhydryl groups, also eliminates 7-8 S generating ability. These observations suggest that a protein may be a component of the 7-8 S generating material. This is substantiated by the observation that trypsin or chymotrypsin treatment of the cytosol mitigates the ability of the cytosol to form the 7-8 S material and results in the appearance of a form of the receptor that sediments at approximately 6 S. Protease treatment of partially purified material eliminates the 7-8 S generating activity entirely. We conclude that the 7-8 S form of the receptor can be reconstituted from the 4 S receptor via association with at least two other cytosolic factors, a protein and an RNA.

Animals↗
PubMed1986

Studies with chymotrypsin and RNAase showing a heterooligomeric structure of the glucocorticoid receptor complex from rat liver which is stabilized by a low molecular weight factor.

The glucocorticoid receptor from rat liver displays a differential sensitivity toward digestion by chymotrypsin and RNAase A that is dependent on its activation state. Unactivated (9-10 S) receptor is not digested by these enzymes, while activated 7-8 S receptor is. Chymotrypsin treatment yields an approx. 3 S form, while RNAase treatment yields a 4.9 S form that is distinct from the high-salt 4 S form. To firmly establish that the results are due to specific hydrolytic activities of the particular enzymes, we show that the chymotrypsin effect is inhibited by diisopropylfluorophosphate and not RNAasin, while the reverse is true for RNAase A. We further show that the differential sensitivity toward chymotrypsin is due to the association of a proteinase-resistant, heat-stable low molecular weight factor with the unactivated glucocorticoid receptor. When this factor is removed by warming, dialysis or molecular sieving of the receptor complex, the complex becomes sensitive to chymotrypsin. We also show that moderate chymotrypsin treatment yields a 6-7 S form of the receptor which is composed of, at least, RNA and the 4 S receptor. On the basis of these results, we propose that the 9-10 S receptor is composed of a low molecular weight stabilizing factor whose presence apparently alters the conformation of the complex such that the RNA and the RNA-binding site of the receptor are protected, a chymotrypsin-sensitive factor, RNA and the 4 S receptor itself.

Animals↗
PubMed1985

Stabilization of glucocorticoid receptor association with RNA by a low molecular weight factor from rat liver cytosol.

A small (Mr less than 500) anionic, heat-stable molecule has been identified in rat liver cytosol which prevents the RNase-induced decrease in the glucocorticoid receptor sedimentation properties which we described previously. This factor, which can be removed by dialysis, molecular exclusion chromatography, or ultrafiltration, functions as a true stabilizer of the RNA-glucocorticoid receptor association, and not as a RNase inhibitor. Preliminary characterization shows that the factor is not a protein, nucleic acid, or nucleotide, is not absorbed by activated dextran-charcoal, and is unaffected by extraction with organic solvents. This factor prevents activation of the glucocorticoid receptor by dilution. The relationship of this stabilization factor to a low molecular weight activation inhibitor described by others is discussed.

Animals↗
PubMed1983

Penile prosthesis in Peyronie's disease.

Many patients with Peyronie's disease suffer severe penile curvature, impotence, and respond poorly to conservative management. Sixteen patients with intractable Peyronie's disease had surgical incision of Peyronie's plaque and insertion of semi-rigid or inflatable penile implants. Patients had satisfactory results with elimination of curvature and resumption of coitus. Surgical procedures are indicated for impotent patients with Peyronie's disease who are unresponsive to conservative treatment.

Adult↗
PubMed1982

Long-term results with the Kaufman prosthesis.

Patients with incontinence after prostatectomy were examined for long-term results following implantation of a Kaufman prosthesis. These appears to be a dichotomy between patients who underwent implantations of the device after radical procedures versus those who had been operated upon for benign disease. Patients undergoing implantation of the prosthesis after an operation for malignant disease experienced a higher rate of complications and a lower over-all continence rate. Also, patients who underwent implantation of the Kaufman prosthesis in conjunction with the Small-Carrion prosthesis showed a 100 per cent failure rate. It appears from the data that the Kaufman prosthesis is not the long sought panacea.

Humans↗
PubMed1981

Identification of ureteroileal obstruction by retrograde loopography.

Retrograde loopography in 106 patients (212 renal units) with ileal conduit urinary diversion was assessed to define the accuracy of this study in identifying ureteroileal obstruction. In 171 renal units (81%), the radiographic findings demonstrated by retrograde loopography correlated appropriately with the findings on excretory urography. However, in 41 renal units (19%) with absent ileoureteral reflux, there was no evidence of ureteroileal obstruction by excretory urography. This potential disparity between excretory urography and retrograde loopography has limited our use of the latter study for selected patients in whom the excretory urogram demonstrates (1) evidence of progressive upper tract deterioration, (2) anastomotic obstruction, or (3) patients with chronic renal failure or iodide sensitivity in whom the intravenous administration of contrast material may be contraindicated.

Adolescent↗
PubMed1981

The primary megacystis syndrome.

We discuss 7 cases of the primary megacystis syndrome to demonstrate that the syndrome is non-obstructive and non-neurogenic, and probably of psychogenic origin owing to learned dysfunctional voiding. The major complication is vesicoureteral reflux and initial therapy is aimed at its correction. Prolonged followup and retraining of voiding habits are necessary. Specific pharmacologic or biofeedback techniques are indicated should urodynamic abnormalities of function be evident.

Child↗
PubMed1981

Methylphenidate compared with behavioral self-control in attention deficit disorder: preliminary report.

The effects of methylphenidate and behavioral self-control training on sustained attention were compared in 12 boys aged 6-9 years. Subjects were of low-average intelligence and had academic and behavioral problems including distractibility, short attention span, and impulsiveness. Treatment conditions (baseline, placebo, methylphenidate, and behavioral self-control training) were assigned according to a single-subject randomized blocks design. Attention was measured by the Children's Checking Test (CCT). Methylphenidate improved performance on the CCT and was superior to the other treatment conditions. Several children benefited from behavioral training, but statistical analysis of this change was nonsignificant. These results are consistent with and extend those of previous studies.

Attention↗
PubMed1980

Bilateral adrenal neuroblastoma.

Bilateral adrenal neuroblastoma is extremely rare, with isolated case reports considered to represent metastatic spread rather than simultaneous occurrence. This patient was found to have synchronous, separate, and equal size neoplasms that morphologically and ultrastructurally are neuroblastoma. We believe that these findings represent the unusual occurrence of simultaneous primary adrenal neuroblastoma reflecting multicentric origin of this neoplasm.

Adrenal Gland Neoplasms↗
PubMed1980

Staging pelvic lymphadenectomy for carcinoma of the prostate risk versus benefit.

A total of 125 patients underwent limited staging lymphadenectomy for adenocarcinoma of the prostate. Of these patients 19 per cent with clinical stage B1 disease, 52 per cent with clinical stage B2 disease and 59 per cent with clinical stages C disease had positive nodes. There was 1 death secondary to myocardial infarction and postoperative complications occurred in 7.2 per cent of the 125 patients. Limited staging pelvic node dissection provides information regarding nodal dissection similar to that identified after more extensive dissection and it can be accomplished with little morbidity.

Adenocarcinoma↗
PubMed1979

Complications of Small-Carrion penile prosthesis.

Seventy-six patients with impotence have undergone insertion of the Small-Carrion penile prosthesis at Duke University Medical Center. Twenty patients experienced postoperative complications. Seven of these 20 patients lost one or both prostheses either by spontaneous extrusion or surgical removal. Although success with this procedure is well documented, complications may be significant when they occur and awareness of these problems is essential for proper management. The benefits of this procedure are reviewed, and reported complications and their management are discussed.

Erectile Dysfunction↗
PubMed1979

Simultaneous open prostatectomy and inguinal herniorrhaphy.

We report our results in 18 patients who had simultaneous open prostatectomy and inguinal herniorrhaphy between 1969 and 1976, and present our criteria for patient selection, operative technic, and postoperative results. The incidence of postoperative wound infection and recurrent hernia was 5.6% and 0 respectively, which compares favorably to results of herniorrhaphy and prostatectomy performed separately. The historic development of this procedure and the results of previously reported series are reviewed.

Hernia, Inguinal↗