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Biomedical subjects

E E Allen

Publications and source records attributed to E E Allen.

17 recordsLinked to original sources
PubMed2003

The genome of a motile marine Synechococcus.

Marine unicellular cyanobacteria are responsible for an estimated 20-40% of chlorophyll biomass and carbon fixation in the oceans. Here we have sequenced and analysed the 2.4-megabase genome of Synechococcus sp. strain WH8102, revealing some of the ways that these organisms have adapted to their largely oligotrophic environment. WH8102 uses organic nitrogen and phosphorus sources and more sodium-dependent transporters than a model freshwater cyanobacterium. Furthermore, it seems to have adopted strategies for conserving limited iron stores by using nickel and cobalt in some enzymes, has reduced its regulatory machinery (consistent with the fact that the open ocean constitutes a far more constant and buffered environment than fresh water), and has evolved a unique type of swimming motility. The genome of WH8102 seems to have been greatly influenced by horizontal gene transfer, partially through phages. The genetic material contributed by horizontal gene transfer includes genes involved in the modification of the cell surface and in swimming motility. On the basis of its genome, WH8102 is more of a generalist than two related marine cyanobacteria.

Bacterial Proteins↗
PubMed2000

Biotechnology, inquiry, and public education.

Education of our children is arguably society's most important task, profoundly shaping the communities in which we all live. Achievement and success in many facets of our culture depend critically on formal education. Education is widely perceived as the only viable weapon against the poverty, drug abuse, crime and teenage pregnancy that derail many citizens, particularly in the inner cities, from realizing their productive human potential. Beyond its value to individuals, education is the cornerstone of societal advancement.

Biotechnology↗
PubMed2000

FabF is required for piezoregulation of cis-vaccenic acid levels and piezophilic growth of the deep-Sea bacterium Photobacterium profundum strain SS9.

To more fully explore the role of unsaturated fatty acids in high-pressure, low-temperature growth, the fabF gene from the psychrotolerant, piezophilic deep-sea bacterium Photobacterium profundum strain SS9 was characterized and its role and regulation were examined. An SS9 strain harboring a disruption in the fabF gene (strain EA40) displayed growth impairment at elevated hydrostatic pressure concomitant with diminished cis-vaccenic acid (18:1) production. However, growth ability at elevated pressure could be restored to wild-type levels by the addition of exogenous 18:1 to the growth medium. Transcript analysis did not indicate that the SS9 fabF gene is transcriptionally regulated, suggesting that the elevated 18:1 levels produced in response to pressure increase result from posttranscriptional changes. Unlike many pressure-adapted bacterial species such as SS9, the mesophile Escherichia coli did not regulate its fatty acid composition in an adaptive manner in response to changes in hydrostatic pressure. Moreover, an E. coli fabF strain was as susceptible to elevated pressure as wild-type cells. It is proposed that the SS9 fabF product, beta-ketoacyl-acyl carrier protein synthase II has evolved novel pressure-responsive characteristics which facilitate SS9 growth at high pressure.

3-Oxoacyl-(Acyl-Carrier-Protein) Synthase↗
PubMed1999

Monounsaturated but not polyunsaturated fatty acids are required for growth of the deep-sea bacterium Photobacterium profundum SS9 at high pressure and low temperature.

There is considerable evidence correlating the production of increased proportions of membrane unsaturated fatty acids (UFAs) with bacterial growth at low temperatures or high pressures. In order to assess the importance of UFAs to microbial growth under these conditions, the effects of conditions altering UFA levels in the psychrotolerant piezophilic deep-sea bacterium Photobacterium profundum SS9 were investigated. The fatty acids produced by P. profundum SS9 grown at various temperatures and pressures were characterized, and differences in fatty acid composition as a function of phase growth, and between inner and outer membranes, were noted. P. profundum SS9 was found to exhibit enhanced proportions of both monounsaturated (MUFAs) and polyunsaturated (PUFAs) fatty acids when grown at a decreased temperature or elevated pressure. Treatment of cells with cerulenin inhibited MUFA but not PUFA synthesis and led to a decreased growth rate and yield at low temperature and high pressure. In addition, oleic acid-auxotrophic mutants were isolated. One of these mutants, strain EA3, was deficient in the production of MUFAs and was both low-temperature sensitive and high-pressure sensitive in the absence of exogenous 18:1 fatty acid. Another mutant, strain EA2, produced little MUFA but elevated levels of the PUFA species eicosapentaenoic acid (EPA; 20:5n-3). This mutant grew slowly but was not low-temperature sensitive or high-pressure sensitive. Finally, reverse genetics was employed to construct a mutant unable to produce EPA. This mutant, strain EA10, was also not low-temperature sensitive or high-pressure sensitive. The significance of these results to the understanding of the role of UFAs in growth under low-temperature or high-pressure conditions is discussed.

Cell Membrane↗
PubMed1992

A conjecture of Procesi and the straightening algorithm of Rota.

Let R = Q[x1, x2,..., xn] and R* denote the quotient of R by the ideal generated by the elementary symmetric functions. R*, under the action of Sn, yields a graded version of the left regular representation. Procesi asked for a basis of R* consisting of homogeneous polynomials Gamma[S, C] indexed by pairs of tableaux, with S standard and C cocharge, that exhibits the decomposition of R* into its irreducible components. Procesi also suggested a way to construct the Gamma[S, C]. Using Rota's straightening algorithm, I show that certain polynomials [S, C] closely related to the Gamma[S, C] terms yield the desired basis. Parallel to the ring R* there is a family of Sn-modules R that have recently been studied by Garsia and Procesi. These modules have a graded character that is closely related to the q-Kostka-Foulkes polynomials Klambdamu(q). The [S, C] can be shown to yield also a basis when restricted to a given R. Through this connection the work reported here leads to an additional way of proving the charge interpretation for the polynomials Klambdamu(q).

Journal Article↗
PubMed1992

Sexual behavior and 2-deoxyglucose uptake in male red-sided garter snakes (Thamnophis sirtalis parietalis).

The [14C]2-deoxyglucose (2-DG) technique was used to study patterns of neural activity associated with the species-typical courtship behavior of male red-sided garter snakes (Thamnophis sirtalis parietalis). Males in this species court females intensely during the first month following spring emergence from their prolonged winter hibernation. Autoradiographic methods were used to measure the accumulation of radioactive label in various regions through the brains of male garter snakes that courted females, males that failed to court females, and males not exposed to females. Male garter snakes that actively courted females showed a pronounced increase in 2-DG accumulation, and therefore presumably neural activity, in the region of the anterior hypothalamus/preoptic area, relative to males that did not actively court females. Males exposed to females (regardless of whether they courted or not) showed widespread, non-specific increases in 2-DG uptake relative to males not exposed to females. The results indicate the utility of the 2-DG technique for studying complex, species-typical behaviors in vertebrates.

Animals↗
PubMed1991

Evaluation of immunoassay methods for the screening of cocaine metabolites in urine.

Immunoassay kits for urine cocaine (and metabolite) screening, obtained from two commercial sources, were examined for correlation of their results, expressed in terms of equivalent benzoylecgonine concentration, with the gas chromatography/mass spectrometry (GC/MS) concentration of benzoylecgonine. The correlation coefficients obtained, based on 62 (out of a total sample population of 3295) highly relevant samples, were 0.467 and 0.766 for Abuscreen (ARIA) and TDx (TDX), respectively. The preliminary screen cutoff values, which correspond to 150 ng/mL benzoylecgonine (as determined by GC/MS), were calculated based on the resulting regression equations and found to be 380 and 190 ng/mL for ARIA and TDX, respectively. With these cutoff values, ARIA generates 5 false negatives and 16 unconfirmed presumptive positives, while TDX results in 3 false negatives and 6 unconfirmed presumptive positives.

Binding, Competitive↗
PubMed1989

2-Deoxyglucose uptake following visual stimulation in squamate reptiles.

Unilateral visual stimulation following 2-deoxyglucose injection was used to delineate functional visual pathways in red-sided garter snakes (Thamnophis sirtalis parietalis) and whiptail lizards (Cnemidophorus inornatus and C. uniparens). Stimulation enhanced both overall uptake and particularly uptake in visual pathways contralateral to the side of stimulation. Bilaterally symmetrical uptake was observed in retinae, septum, nucleus sphericus, and brainstem nuclei. Overall uptake and lateralization of uptake were consistently greater in garter snakes than in whiptail lizards.

Animals↗
PubMed1988

6-Hydroxydopamine treatment and beta adrenergic receptor binding in kittens. Relation to visual cortical plasticity.

Under some circumstances intraventricular administration of 6-OHDA decreases visual cortical plasticity of kittens; the mechanism for this change is not known, but depletion of norepinephrine (NE) is not the entire explanation. We have examined the effects of 6-OHDA treatment on beta adrenergic receptor binding in kitten visual cortex. Subjects were given vehicle solution alone, a low dose of 6-OHDA which depleted cortical NE without affecting visuocortical plasticity, or a higher dose of 6-OHDA which depleted cortical NE and decreased visuocortical plasticity. Drugs were administered in single daily injections via intraventricular cannulas. Saturation assays were performed on homogenates of visual cortical tissue using 125I-pindolol (30-400 pM) along w/ isoproterenol (237 microM) as a cold competitor. We measured radioactivity bound to tissue and retained on filters and analyzed the data using the EBDA computer program (McPherson 1983, 1985); we determined the affinity constant (Kd) and receptor density (Bmax) in multiple assays for each animal. Despite 75-90% NE depletion in both experimental groups, only the group receiving the lower dose of 6-OHDA showed any evidence of supersensitivity. The Kds did not differ among the groups. The data suggest that the effects of 6-OHDA on visuocortical plasticity are not secondary to beta adrenergic supersensitivity.

Animals↗
PubMed1987

Effect of desmethylimipramine on norepinephrine content and plasticity of kitten visual cortex.

Because norepinephrine (NE) reuptake inhibitors have been reported to block 6-hydroxydopamine (6-OHDA) induced NE depletion, we wondered whether a reuptake inhibitor would block 6-OHDA's effects on visual cortical plasticity. We found, however, that desmethylimipramine (DMI) did not reduce 6-OHDA-induced NE depletion at 6-OHDA doses sufficient to prevent the effects of monocular deprivation. We also found that DMI itself induced transient NE depletion. We used this last result to further examine the NE hypothesis of depletion. In contrast to 6-OHDA-induced NE depletion, DMI-induced NE depletion was not accompanied by changes in visual cortical plasticity.

Animals↗
PubMed1987

Timing of 6-hydroxydopamine administration influences its effects on visual cortical plasticity.

We recorded from the visual cortex of 4 groups of monocularly deprived kittens. Three groups were treated with intraventricular 6-hydroxydopamine (6-OHDA) at different times relative to monocular deprivation (MD). One group received only vehicle solution and MD. 6-OHDA caused the greatest decrease in plasticity in the kittens receiving 6-OHDA throughout the deprivation period; that is, these kittens were the least affected by MD. 6-OHDA caused a smaller decrease in plasticity in kittens receiving 6-OHDA just prior to eyelid suture and a still smaller decrease in kittens waiting a week between 6-OHDA treatment and eyelid suture. The kittens in all groups receiving 6-OHDA were equally depleted of norepinephrine (NE). We conclude that 6-OHDA decreases plasticity in the visual cortex; however, the time course of this decrease is better related to the time course of the 6-OHDA treatment than to the time course of NE depletion.

Age Factors↗
PubMed1986

Doses of 6-hydroxydopamine sufficient to deplete norepinephrine are not sufficient to decrease plasticity in the visual cortex.

These experiments were designed to test Kasamatsu and Pettigrew's (1979, 1983, and see below) hypothesis that plasticity in the visual cortex requires cortical norepinephrine (NE). Kittens were treated with various doses of intraventricular 6-hydroxydopamine (6-OHDA) or vehicle solution. Cortical NE content was measured with high-performance liquid chromatography with electrochemical detection. We sutured the right eyes of some kittens approximately 6 weeks of age for 1 week and recorded from the left visual cortex of these kittens at the end of the week of suture. We measured the ability of the deprived eye to drive cortical cells in animals that received either 0.2 or 4.8 mg of 6-OHDA, and also in control animals that received only vehicle solution. We concluded that a particular dose of 6-OHDA decreased plasticity if it increased (relative to controls) the ability of the deprived eye to drive cortical cells. Doses of 6-OHDA as small as 0.2 mg were sufficient to produce approximately maximal depletion of NE but did not decrease cortical plasticity. Doses of 4.8 mg or more did decrease cortical plasticity, although not as much as was reported by Kasamatsu and Pettigrew. We conclude that 6-OHDA can alter cortical plasticity but the decrease in plasticity does not result from NE depletion.

Animals↗
PubMed1985

Spectral sensitivity of the African cichlid fish, Haplochromis burtoni.

Spectral sensitivity of the cichlid fish Haplochromis burtoni was measured under both scotopic and photopic conditions using a two-choice, food reward, operant conditioning paradigm. The highest absolute sensitivity (scotopic) is one quantum for every 5 to 50 rods measured at 475 nm (equivalent to a corneal irradiance of 3.8 x 10(6) Q s-1 cm-2). A P500(1) photopigment apparently mediates spectral sensitivity over most of the visible spectrum; microspectrophotometric studies of rods had previously shown them to contain this photopigment. However, the scotopic behavioral action spectrum shows a sensitivity to short wavelength light higher than is consistent with a P500(1) photopigment alone mediating the scotopic visual process. Determinations made under photopic conditions reveal a behavioral action spectrum broader than that found under scotopic conditions and consistent with mediation by interaction of the three known cone types in an opponent processing manner. The calculated photopic threshold value of approximately 10(4) Q s-1 (receptor)-1 is in agreement with results from other species and corresponds to a corneal irradiance of about 7 x 10(10) Q s-1 cm-2.

Animals↗
PubMed1978

Thalamic projections to S-I in macaque monkey.

The organization of thalamic input to functionally characterized zones in primary somatosensory cerebral cortex (S-I) of macaque monkeys (Macaca mulatta) was investigated using the method of labelling by retrograde transport of horseradish peroxidase (HRP). It was found that the cell columns positioned at the posterior margin of the band of cortex representing a given body region receive thalamic input from a posterior level of the ventroposterior thalamic nucleus (VP), and that cell columns at successively more anterior positions within that band receive input from successively more anterior levels of VP. The extreme posterior and anterior margins of the S-I hand, foot and face areas receive input from neuron populations which are not as widely separated in the anteroposterior dimension of VP as the neurons projecting to the extreme anterior and posterior margins of the proximal limb and trunk representations in S-I. These characteristics of the organization of the projections from VP to S-I are consistent with the view that the body representations in VP and S-I have the same connectivity and differential submodality distribution; and with the idea that thalamocortical conncetions only exist between functionally equivalent neuron populations in VP and S-I.

Adaptation, Physiological↗