Search PubMed⌕ Search

Biomedical subjects

E Durante

Publications and source records attributed to E Durante.

At least 19 recordsLinked to original sources
PubMed1995

Primary cultures of human hypertrophic prostate tissue in WAJC 404 medium: a study of cell morphology and kinetics.

222 biopsy fragments of human hypertrophic prostate tissue were cultured in WAJC 404 serum-free medium for three weeks. Growth halos were examined after 7, 14 and 21 days of culture by optical and scanning electron microscopy. Colonies formed of two concentric areas showed in the inner halo elementary pseudo-lobular morphological units similar to the prostate structure. The cell morphological patterns of the halo turned out to be four in number. Every cell pattern was defined morphologically, morphometrically and phenotypically. Results indicate that all morphological differences must be attributed to the various phases of cell life, as all cell types were positive to cytokeratin. The nonconstant display of PSAP and PSA showed a moderate tendency to cell differentiation in WAJC 404 medium. Cell kinetics were also studied and revealed a decrease in proliferation after 14 days of culture. Primary cultures from biopsy fragments of human hypertrophic prostate tissue may be used as an experimental model up to the 14th day of culture.

Aged↗
PubMed1993

Experimental vermeersiekte (Geigeria ornativa O. Hoffm. poisoning) in sheep. I: An evaluation of diagnostic aids and an assessment of the preventive effect of ethoxyquin.

Dried milled Geigeria ornativa, which had been stored at room temperature for 4 years, was fed in rations to sheep. The usefulness of contrast radiography of the oesophagus, biopsies of oesophageal and skeletal muscles, electrocardiography, and clinical pathological parameters for diagnosing vermeersiekte were evaluated. Ethoxyquin was evaluated as a preventive agent. All the sheep developed signs of vermeersiekte but regurgitation was observed in only one animal. Contrast radiography and the examination of tissue biopsies were of diagnostic value in 8 out of 13 and 6 out of 6 sheep respectively. Electrocardiography did not aid in antemortal diagnosis of the condition and selected chemical parameters were non-specific. The inclusion of ethoxyquin in the diet of experimental animals (n = 3) prior to and during feeding of G. ornativa did not prevent the development of vermeersiekte. This study confirmed that dried stored G. ornativa retains its toxicity and that regurgitation is not a consistent clinical sign in sheep with vermeersiekte. The diagnostic value of contrast radiography of the oesophagus and examination of oesophageal and muscle biopsies is reported for the first time.

Animals↗
PubMed1990

[I.V. ketorolac in the therapy of postoperative pain].

Forty-one patients suffering from postoperative pain were admitted to an open, noncomparative trial. A dose of 10 mg of Kerotolac was administered i.v. to patients complaining severe pain. The analgesic efficacy of the drug was evaluated at 5, 15, 30 minutes and at 1, 1.5, 2, 3, 4, 6 hours after injection; the patients were asked to score their pain relief on the VRS comparing the pre-injection evaluation to each post-injection period. A significant decrease of pain intensity (less than p 0.01) was observed at 45 minutes after injection. No side effects were observed. These results suggest that Ketorolac can be successfully used in the treatment of postoperative pain.

Adolescent↗
PubMed1986

Anaplastic adenocarcinoma of the thyroid gland: a study of ten cases.

Anaplastic carcinoma of thyroid gland, while rare, is the most common cause of death from thyroid cancer. Ten cases from a teaching department of surgery have been reviewed, with two-year follow up. Steps in pathological behaviour and management of this lesion are discussed. From the review, it seems that the only logical option in the treatment of anaplastic carcinoma of thyroid gland, is total thyroidectomy.

Adenocarcinoma↗
PubMed1984

[New prospects offered by echography in the diagnosis of carcinoma of the gallbladder].

Experience in diagnosing several cases of primary carcinoma of the cholecyst echographically in reported. The most important ultrasonic characteristics in relation to staging of the lesion are reviewed. It is seen that echographic features always correlate with surgical findings, confirming the diagnostic accuracy of the method which permits morphofunctional as well as structural evaluation. At this time echography seems to be the most rational tool for efficient diagnosis, a step forward in ensuring timely surgery and the improvement of long term prospects for the condition.

Adenocarcinoma↗
PubMed1982

[Scope and limitations of the surgical treatment of diabetes].

A brief account is given of the current knowledge, experiments, and clinical findings concerning the surgical management of diabetes. The aim of all treatments, from conventional pancreas transplant to the sophisticated transplantation of islets and foetal pancreas, and a subject with its own special features, namely intestinal by-pass, is to relieve the patient from insulin management, which, while it increases his survival, does not defend him from possibly fatal vascular complications. Though far from providing a final solution, each of the topics dealt with provides an example of the way research and clinical practice continue to offer reasons for the experimentation of new therapeutic techniques.

Diabetes Complications↗
PubMed1981

[Correlation between hyperamylasemia and acute pancreatitis].

It is often difficult to differentiate acute pancreatitis (A.P.) from some other acute abdominal diseases, when there is an elevated serum amylase. In contrast, the renal clearance of amylase, expressed as a percentage of creatinine clearance, can separate patients with A.P. from patients with acute colecistitis, common duct stone without pancreatitis, hyperamylasemia after biliary surgery, acute peptic ulcer and acute salivary diseases.

Acute Disease↗
PubMed1980

[Echography in renal tumors].

Echography is highly important today for the identification and differentiation of renal diseases. It also enable target biopsy of identified lesions to be carried out, thus making for precise histological diagnosis. Indications and results of echography in the diagnosis of 97 cases of suspect masses of renal origin consisting of cysts, hydropyonephrosis, tumours, multicystic and polycystic kidneys are considered. Overall diagnostic accuracy was 92.7%. The features pointing to differential diagnosis of individual lesions and the method of examination, based on the use A and B Scan with grey scale are described. It is concluded that, with their high diagnostic accuracy and non-invasivity, scanning techniques represent an indespensable tool for the diagnosis of renal masses.

Humans↗
PubMed1979

[Clinical and instrumental study program of nodular diseases of the thyroid].

Authors have passed from a combined clinical and radioisotopic analysis to an integrated polyinstrumental approach by adding thermography and above all echography. In order to achieve the purpose Authors have confronted in retrospective a "clinico-radioisotopic group" of 103 cases, from 1965 to 1975, and an "integrated polyinstrumental group" of 53 cases, since 1976. Comparing the "clinico-radioisotopic method" with "polyinstrumental approach" the main results could be summarized as follows: the diagnostic specificity improved in benign lesions from 77% to 96%, however in malignant lesions it remained about the same (63%). Considering these results the Authors have subdivided thyroid nodules, mainly based on echography, in two distinct categories with the following orientation: I. Solitary Thyroid Nodule: a) definitely cystic -- demonstrated by echography; b) "cold" -- by conventional scintygraphy; c) "negative" -- by thermography; d) non uptake of tumor seeking radiopharmaceutical. The control, therapy and surveilance of these cases should be limited to needle aspiration of the cystic cavity followed, of course, by citologic examination. This aspirations is both diagnostic and possibly therapeutic. II. Solitary Thyroid Nodule: a) definite solid -- echography; b) "cold" -- convential scintygraphy; c) "positive" --thermography (possibly "negative"); d) non uptake of tumor seeking radiopharmaceutical. Here the therapeutic orientation is clearly surgical.

Cysts↗
PubMed1979

[Current status in transplantation of the endocrine pancreas in the treatment of diabetes mellitus 2. Transplantation of fetal pancreas].

The latest findings support hypotheses that diabetes complications are secondary to metabolic control which current insulin therapy is unable to render constant. Surgical attempts to transplant the pancreas and Langerhans islets in the treatment of diabetes mellitus are reviewed and special attention paid to the use of the foetal pancreas which has proved particularly suitable in preventing microangiopathic complication. Comments are based on a careful review of the literature, made in the light of personal experience.

Animals↗