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Biomedical subjects

E Dabelsteen

Publications and source records attributed to E Dabelsteen.

At least 127 records · Page 7Linked to original sources
PubMed1985

Langerhans cells in candidal leukoplakia.

Using histochemical and immunohistochemical methods, we examined specimens of candidal leukoplakia from the oral mucosa of 5 smokers to determine the morphological relationships between Candida and Langerhans cells (LC) in tissue sections. LC were fairly evenly distributed in control sections, but had a patchy distribution in lesions. Fewer LC were found in lesions than in control tissue, but the difference was not statistically significant. Candidal antigens were not detected on LC by the methods used, but we found ATPase-positive LC among, or at least near, intraepithelial candidal hyphae. However, sections double-reacted with anti-Candida and T6 antibodies to label candidal antigens and LC, respectively, showed a clear zone of epithelium between the T6-positive LC and the candidal hyphae. The difference in distribution of ATPase-positive and T6-positive LC may indicate locations of 2 subtypes of LC, or a change in T6 antigen expression by the LC closest to the candidal hyphae.

Adult↗
PubMed1985

Pattern of distribution of T lymphocytes, Langerhans cells and HLA-DR bearing cells in normal human oral mucosa.

The tissue distribution of helper/inducer and suppressor/cytotoxic T cells, Langerhans cells (LC) and HLA-DR bearing cells was determined in normal oral mucosa by use of monoclonal antibodies OKT4, OKT8, OKT6 and OKIa1, respectively. OKT4+ and OKT8+ cells were invariably present in normal oral epithelium and in the lamina propria. OKT8+ cells were consistently seen inside the basal cell layer of the epithelium. The distribution of LC in oral epithelium showed regional variation. In palatal epithelium LC were evenly distributed in the basal half of the epithelium, whereas in buccal mucosa the highest concentration of LC was seen in the epithelium overlying the tips of connective tissue papillae. OKIa1 stained dendritic cells in the epithelium and plump cells with small dendritic processes in the connective tissue. Some of the latter were located close to the basal cells of the epithelium. The consistent relationship between immunocompetent cells and the epithelium of the oral mucosa suggests the presence of a local immunologic defence barrier in the oral mucosa.

Adolescent↗
PubMed1985

Changes of blood group antigens in premalignant and malignant lesions of the human exocervix.

In this preliminary study the distribution of blood group A- and H-antigens and their structural precursor N-acetyllactosamine in normal, pre-malignant and malignant squamous epithelium were investigated using the immunofluorescence technique. In normal cervical epithelium the basal cells and few parabasal cells expressed N-acetyllactosamine. The parabasal and lower spinous cells expressed the H-antigen and the upper spinous layer expressed the A-antigen. All pre-malignant and malignant lesions showed irregular decrease in contents of A- and H-antigens. N-acetyllactosamine was either completely lost or found to accumulate in these lesions, and it is suggested that this antigen could be a valuable tool in the diagnosis of cervical cancer.

ABO Blood-Group System↗
PubMed1985

Staining patterns of oral lesions of discoid lupus erythematosus by monoclonal anti-keratin antibodies.

The immunofluorescent staining patterns of oral lesions of discoid lupus erythematosus were examined by use of monoclonal antibodies AE1, AE2 and AE3. AE1 and AE2 showed suprabasal staining, whereas AE3 stained all cell layers of the epithelium. This pattern is consistent with that of other benign hyperkeratinized lesions of the oral mucosa. Occasionally, however, the most basally positioned epithelial cells stained positive with AE1. The morphology of these cells was similar to stratum spinosum cells. Colloid bodies in the epithelium as well as in the connective tissue stained positive with AE1 and AE3, demonstrating their epithelial origin. Few bodies in the connective tissue staining positive with IgM were negative for keratin. These structures may be Russell bodies or may be derived from the basement membrane zone.

Antibodies, Monoclonal↗
PubMed1985

Staining patterns of human pre-malignant oral epithelium and squamous cell carcinomas by monoclonal anti-keratin antibodies.

Formalin-fixed, paraffin-embedded biopsies of metaplastic keratinized oral mucosa (fibromas and leukoplakias), oral mucosa with epithelial dysplasia and oral squamous cell carcinomas were stained with two monoclonal anti-keratin antibodies (AE1 and AE2). Intense suprabasal staining was seen with AE1 in metaplastic keratinized epithelium, whereas staining of adjacent normal unkeratinized epithelium generally was restricted to basal cells. In dysplastic epithelium and squamous cell carcinomas, staining with AE1 revealed a highly disturbed anti-keratin staining pattern. AE2 stained metaplastic keratinized epithelium in a suprabasal pattern but adjacent unkeratinized epithelium did not stain. In dysplastic epithelium and squamous cell carcinomas, AE2 staining was variable and sometimes absent. Further studies are indicated to clarify whether changes in anti-keratin staining patterns can be used for diagnostic and prognostic purposes.

Antibodies, Monoclonal↗
PubMed1985

Keratin proteins in the epithelium of the uterine cervix.

Keratin proteins in human cervical squamous epithelium were investigated by the immunofluorescence technique, using the monoclonal keratin antibodies AE1 and AE2. Although the exocervic is covered by a non-keratinizing, histologically homogenous, squamous epithelium, different staining patterns were found within a single histological section. This indicates a regional variation in the maturation pattern of stratified exocervical epithelium. Detection of morphological versus biochemical keratinization in the diagnosis of pathological cervical conditions is discussed.

Antibodies, Monoclonal↗
PubMed1985

EDTA separation and recombination of epithelium and connective tissue of human oral mucosa. Studies of tissue transplants in nude mice.

A possible epithelial-mesenchymal interaction in determining epithelial histologic features of human oral mucosa was examined. The study comprised 74 biopsies of normal buccal mucosa and 54 biopsies of normal palatal mucosa. Epithelium was separated from connective tissue by the use of 1 mM ethylenediamine tetraacetate dihydrate. Self-recombined and cross-recombined epithelial and connective tissues and connective tissue sheets alone were transplanted to subcutaneous sites of nude mice. Histologic examination of cross-recombined palatal epithelium/buccal connective tissue transplants showed a change in keratinization pattern but no major change in number of epithelial cell layers as the result of connective tissue influence. Transplanted sheets of connective tissue after growth for 14 days showed that complete separation of biopsies from buccal mucosa had been obtained. However, palatal mucosa had been incompletely separated as evidenced by re-epithelialization of most of the connective tissue transplants. The consequences of the incomplete palatal epithelium-connective tissue separation are discussed.

Adolescent↗
PubMed1984

The distribution of blood group antigens in rodent epithelia.

The pattern of distribution of antigens cross-reacting with antibodies to human blood group antigens A and B and two precursor molecules was examined by immunofluorescence in the epidermis, oral mucosa and forestomach of rats and mice. Staining for blood group antigen A was negative. In all epithelia examined, blood group antigen B was present at the surface of basal and parabasal cells, and the H antigen at the surface of spinous cells. N-acetyllactosamine was present on the cell membranes in the upper spinous and granular cell layers of epidermis and forestomach epithelium and was not expressed in the oral epithelia except for a limited area in the dorsal tongue epithelium. Thus, the expression of antigen varies both regionally and, as earlier shown in human epithelium, with the stage of maturation of cells within a given epithelium. The observed sequence of expression of these antigens during maturation differs from that of human epithelia, but the present study provides a basis for further experimental studies of the role of cell surface antigens in epithelial homeostasis and maturation.

ABO Blood-Group System↗
PubMed1984

Pattern of distribution of blood group antigens on human epidermal cells during maturation.

The distribution in human epidermis of A, B, and H blood group antigens and of a precursor carbohydrate chain, N-acetyl-lactosamine, was examined using immunofluorescence staining techniques. The material included tissue from 10 blood group A, 4 blood group B, and 9 blood group O persons. Murine monoclonal antibodies were used to identify H antigen (type 2 chain) and N-acetyl-lactosamine. Human antisera were used to identify A and B antigens. In all groups N-acetyl-lactosamine and H antigen were found on the cell membranes of the spinous cell layer. N-acetyl-lactosamine was present mainly on the lower spinous cells whereas H antigen was seen predominantly on upper spinous cells or on the granular cells. Epithelia from blood group A or B persons demonstrated A or B antigens, respectively, but only if the tissue sections were trypsinized before staining. In such cases A or B antigens were found on the cell membranes in the granular cell layer.

ABO Blood-Group System↗
PubMed1984

Regional variations of cell surface carbohydrates in human oral stratified epithelium.

The distribution of blood group carbohydrate chains with antigen A, B, H type 2 chain (A and B precursor), and N-acetyllactosamine (H type 2 precursor) specificity was studied in human oral epithelium from different anatomical regions. These represented various epithelial differentiation patterns such as non-keratinized, parakeratinized, and orthokeratinized stratified squamous epithelium. The material included buccal and palatal epithelium from 20 persons with blood group A or O, gingival, and alveolar epithelium from 10 persons with blood group A or B, and buccal metaplastically keratinized epithelium from nine blood group A, two blood group B, and nine blood group O individuals. The blood group carbohydrate chains were examined in tissue sections by immunofluorescence microscopy. The A and B blood group antigens were detected by human blood group sera, and antigen H type 2 chains and N-acetyllactosamine by murine monoclonal antibodies. Each antigen showed a similar staining pattern in buccal and alveolar epithelium (non-keratinized) which differed considerably from that seen in palatal and gingival epithelium (ortho- and parakeratinized). The expression of blood group antigens A or B and the precursor antigen H type 2 chains in metaplastically keratinized buccal epithelium was found to differ significantly from that seen in normal non-keratinized buccal epithelium. The regional variations demonstrated in cell surface carbohydrates are suggested to reflect differences in tissue differentiation.

ABO Blood-Group System↗
PubMed1984

Changes in the expression of blood-group carbohydrates during oral mucosal development in human fetuses.

The distribution of the blood-group antigens A, B, H type 2 (A and B precursor), and N-acetyllactosamine (H-type-2 precursor) was studied in human fetal oral mucosa from 30 fetuses. Epithelium was examined from the lip, the alveolar ridge and the hard palate in fetuses representing development from week 10-20 in utero. The blood-group carbohydrate chains were examined in tissue sections by immunofluorescence microscopy. The A- and B-blood group antigens were detected by human blood-group sera, and antigen-H type-2 chains and N-acetyllactosamine were detected by murine monoclonal antibodies. Regional differences in the expression of oral epithelial blood-group carbohydrates occurred during the fetal period. In the labial epithelium that remained unkeratinized, blood-group antigens A and B were present throughout the entire period of fetal development on the cell surface of the spinous and superficial cells, antigen H type 2 was present on parabasal cells, and N-acetyllactosamine was present on basal cells. In the epithelia of the alveolar ridge and the hard palate, the initial uniform staining for blood-group antigens A and B only occurred in the upper cell layers and changed to include a patchy reaction of single cells in the spinous cell layer. The distribution of antigen H type 2 changed simultaneously to include parabasal cells and the entire spinous cell layer.(ABSTRACT TRUNCATED AT 250 WORDS)

ABO Blood-Group System↗
PubMed1984

Identification of human connective tissue in transplant of human oral mucosa in nude mice.

The present study describes a method for identification of connective tissue of human oral mucosal transplants in nude mice. The method was based on the development of a murine antiserum to human fibroblasts. After absorption with murine fibroblasts the antiserum in an immunofluorescence method appeared to react specifically with human connective tissue of frozen sections, whereas the antiserum did not react with murine connective tissue. The antiserum, applied to frozen sections of human oral mucosal transplants in nude mice, could distinguish between human and murine connective tissue in the sections. The ability to distinguish between the two types of tissue was utilized to elucidate a possible relation between epithelial morphology and underlying type of connective tissue. It was found that the formation of rete ridges of transplanted human oral epithelium was dependent on the presence of subepithelial human connective tissue. The method described may be useful for the recognition of human tissue in experimental studies of human transplants to other species.

Adolescent↗
PubMed1984

Blood group substances, T6 antigen and heterochromatin pattern as species markers in the nude mouse/human skin model.

Antibodies to blood group antigens and to human T6 antigen and the DNA-specific fluorochrome D287/170 were applied to human skin transplanted to nude mice and to the surrounding murine skin. It was found that the two epithelia maintained their characteristic, different blood group B antigen distributions and heterochromatin patterns. Furthermore, it was shown that human Langerhans' cells persisted in the grafted epidermis. During an observation period of until 48 weeks after transplantation only one of 32 grafts showed signs of invasion by murine epidermis.

ABO Blood-Group System↗
PubMed1984

Bone resorption in cholesteatomas. An experimental study.

A model for studying different aspects of human cholesteatomas in vivo in the immunodeficient "nude' mice is described. Epithelial-mesenchymal interaction was studied in human cholesteatoma membranes, transplanted to the flank region. Findings of cystic lesions lined by stratified keratinizing epithelium were frequent and the connective tissue reaction were weak. Furthermore human cholesteatomy membranes were transplanted into the middle ear cavity of the "nude' mice. After 21-28 days bone resorption was shown in the labyrinthine capsule, not always with sign of inflammation.

Animals↗
PubMed1983

Keratin pattern in human and buccal and hard palate mucosa.

As a preliminary study the keratin proteins of human oral stratified epithelia from buccal mucosa and hard palate were investigated using SDS-PAGE technique. The keratinized hard palate consistently showed six polypeptides varying in molecular weight from 47 to 67 k daltons, whereas the nonkeratinized buccal epithelium lacked the larger polypeptides and showed three polypeptides with molecular weight from 50 to 56 k daltons. The individual variations in the SDS-gel pattern from eight clinically normal individuals were restricted to minor quantitative differences.

Cheek↗
PubMed1983

Accumulation of a blood group antigen precursor in oral premalignant lesions.

Epithelial cell membrane-bound blood group antigens A and B are lost in premalignant and malignant oral lesions. We now show that this loss in premalignant lesions is accompanied by accumulation of a blood group antigen precursor. The precursor structures were: type 2 chain H-antigen, Fuc alpha 1 leads to 2 Gal beta 1 leads to 4 GlcNAc-R (A and B precursor); and N-acetyllactosamine, Gal beta 1-4GlcNAc-R (H-precursor), in which Fuc is L-fucose, Gal is galactose, and GIcNAc is N-acetyl-D-glucosamine. They were demonstrated in tissue sections by immunohistochemical staining techniques with monoclonal antibodies to H-antigen and N-acetyllactosamine. Precursors were found only on basal and suprabasal cells of normal mucosa. In all nine of ten premalignant lesions, the H-antigen was found on all cell membranes in the epithelium, in higher titers than in normal adjacent epithelium. Ten carcinomas were studied, and all showed an irregular distribution of H-antigen. N-Acetyllactosamine was not found in premalignant or malignant lesions. The accumulation of type 2 chain H-antigen in oral premalignant lesions may prove helpful in early diagnosis of epithelial cancer.

ABO Blood-Group System↗
PubMed1983

Monoclonal antibodies in the diagnosis of epithelial premalignant lesions.

We investigated 14 oral lesions and 10 skin lesions with epithelial dysplasia and 10 oral squamous cell carcinomas by immunofluorescence staining using monoclonal antibodies to two blood group antigen A and B precursor structures: Type 2 chain H-antigen (Fuc alpha 1 leads to 2 Gal beta 1 leads to 4 GlNAc leads to R; A and B precursor) and N-acetyllactosamine (Gal beta 1 leads to 4 GlNAc leads to R; H precursor). Both precursors were in oral epithelium found on the cell membrane of basal cells; in skin they were present on spinous and granular cells. In 12 of the 14 oral lesions the H-antigen was found on all cells throughout the epithelium in higher titers than in normal adjacent epithelium. All 10 carcinomas showed an irregular distribution of H. Some tumor cells did not express H whereas others did. In all 10 skin lesions on the contrary the H-antigen was lost in the dysplastic epithelium. N-acetyllactosamine was found neither in premalignant or malignant oral nor in premalignant skin lesions. The accumulation of Type 2 chain H-antigen in oral premalignant lesions and the loss of the same antigen in skin premalignant lesions may prove helpful in early diagnosis of epithelial malignancy.

ABO Blood-Group System↗