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Biomedical subjects

E D Frank

Publications and source records attributed to E D Frank.

At least 19 recordsLinked to original sources
PubMed1979

The detection of smooth muscle desmin-like protein in BHK21/C13 fibroblasts.

Three distinct proteins, actin (42,000 daltons), the principal form of fibroblast 10 nm filament protein (55,000 daltons), and a protein with a molecular weight of 52,000 and a pI of 5.8 were detected in nonionic detergent-insoluble cytoskeletal and 10 nm filament preparations of control BHK21/C13 and line 9 hamster fibroblasts. Cytoskeletal preparations of other hamster fibroblast cell types, such as NIL8 and primary embryo fibroblasts, contained the 55,000-dalton component but lacked the 52,000-dalton protein. A Rous sarcoma virus transformant of the BHK21/C13 line and an adenovirus transformant of line 9, resembled the NIL8 and other fibroblast types in that they contained only the 55,000- and 42,000-dalton polypeptides. The identity of the 52,000-dalton protein in BHK21/C13 cells was studied. This protein co-electrophoreses on both one- and two-dimensional polyacrylamide gels with the predominant muscle form of 10 nm filament protein. Further, one-dimensional peptide maps of the hamster smooth muscle 10 nm filament protein and the hamster fibroblast 52,000-dalton protein are identical to one another and distinct from the peptide maps of both the 42,000- and the 55,000-dalton components of the fibroblast cytoskeletal preparations. We conclude that BHK21/C13 cells contain both the fibroblast and the muscle form of 10 nm filament protein.

Animals↗
PubMedDate not supplied

Radiography of the ankle mortise.

Radiographers often use methods to position the ankle that produce inaccurate images of the ankle mortise. This article describes a technique using a 15 degree to 20 degree internal-oblique position of the leg and foot to demonstrate the ankle mortise radiographically. A textbook protocol also is included.

Ankle Joint↗
PubMedDate not supplied

Interpleural bupivacaine for postoperative analgesia following cholecystectomy: a randomized prospective study.

The effect of interpleural analgesia on postoperative hospital course was analyzed ina prospective randomized study of patients undergoing cholecystectomy. Control patients were treated in the standard manner with systemic narcotics alone; catheter patients had an interpleural catheter placed at the end of surgery and, in addition, could receive systemic narcotics if necessary. The catheter group received interpleural 0.5% bupivacaine with epinephrine every six hours for a total of four injections. Thirteen patients were in each group. Pain score, pulmonary function and narcotic requirement were measured over the first postoperative day. Catheter patients had a lower average pain score (visual analog scale (VAS), 3.6 versus 5.2), decreased narcotic requirement in the recovery room and improved oxygen saturation (96% versus 93%). However, there was no statistical difference in amount of morphine (catheter, 25 +/- 14 mg; control, 31 +/- 15 mg), number of narcotic injections (catheter, 3.8 +/- 1.5; control, 3.5 +/- 1.5), forced vital capacity (catheter, 44% preoperative control, 41% preoperative), recovery room time (catheter, 129 +/- 54 minutes, control, 117 +/- 39 minutes) or total hospital stay (catheter, 4.1 +/- 0.9 days; control, 3.7 +/- 0.8 days). Analysis of hourly VAS scores following a bolus indicated that the analgesia disappeared within approximately four hours. The mean time to a request for narcotic following a bolus was 4.2 hours (excluding 17 of a potential total of 52 instances when narcotic was not requested at all). Therefore, the duration of pain relief for subcostal incisions using interpleural 0.5% bupivacaine is approximately four hours.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMedDate not supplied

Scatter radiation.

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Absorption↗
PubMedDate not supplied

Publishing a manuscript: manuscript preparation guide for authors. Part II.

Part II presents a guide for manuscript preparation. The guide describes 11 steps necessary to complete a manuscript and is designed to serve several purposes: making the process of having a manuscript published easier for the author and less tedious for the Editorial Review Board; reducing the number of revisions and the revision time; and providing the author with a better chance of getting his or her material published.

Publishing↗
PubMedDate not supplied

Publishing a manuscript: analysis of reviewed manuscripts with discussion. Part I.

Fifty-one manuscripts that were submitted for publication to Radiologic Technology were analyzed. The acceptance, revision, and rejection rates of those manuscripts are discussed. The specific reasons for the rejection and for the major revisions needed for many of the manuscripts are emphasized. Manuscript preparation is also discussed.

Periodicals as Topic↗
PubMedDate not supplied

X-ray tube warm-ups.

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Technology, Radiologic↗
PubMedDate not supplied

Scatter error.

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Humans↗