Search PubMed⌕ Search

Biomedical subjects

E Cercenado

Publications and source records attributed to E Cercenado.

65 records · Page 4Linked to original sources
PubMed1987

Serratia bacteremia.

During a 6-yr period, 146 patients at our institution had Serratia bacteremia (3.8% of the total number of episodes of bacteremia), with an incidence of 1.24/1000 admitted patients. We chose a random group of 50 cases for clinical analysis in the present study. The disease was community-acquired in 8% of the cases and nosocomially-acquired in the remaining 92%. The bacteremia was unimicrobial in 84% and part of a polymicrobial bacteremia in 16% of the episodes. The most frequently isolated species of the Serratia genus was S. marcescens. Portals of entry, in decreasing order of frequency, were: urinary, unknown, respiratory, and surgical wound infections. Clinically, the most frequent finding was fever (100%). Shock occurred in 28% of the patients, and none of our cases showed evidence of disseminated intravascular coagulation. We found 62% of Serratia isolates resistant to gentamicin. Overall mortality was 38% and factors associated with a poor prognosis were: severity of the underlying disease, critical clinical situation at onset of bacteremia, presence in the intensive care unit (I.C.U.), occurrence of shock or polymicrobial bacteremia, portal of entry in the respiratory tract, and inadequate treatment.

Adolescent↗
PubMed1987

Legionella micdadei and Legionella dumoffii monoclonal antibodies for laboratory diagnosis of Legionella infections.

Two different monoclonal antibodies directed against Legionella micdadei and L. dumoffii (Genetic Systems Corp., Seattle, Wash.) were evaluated for their specificity and ability to detect L. micdadei and L. dumoffii in human and animal clinical samples and bacterial isolates in an indirect immunofluorescence assay. All three frozen sputum samples and all three Formalin-fixed sputum and liver samples from patients with culture-documented L. micdadei pneumonia were positive when tested with the L. micdadei monoclonal antibody. A Formalin-preserved lung sample from a patient with culture-documented L. dumoffii pneumonia was positive with its homologous monoclonal antibody. No cross-staining reactions were found with either monoclonal antibody on any of 25 human sputum samples tested from patients without Legionella infections. A total of 66 Legionella strains and 56 non-Legionella strains including 22 Pseudomonas strains and 34 other bacterial strains were studied. No cross-staining reactions were found except in Staphylococcus aureus Cowan 1 ATCC 12598. The lower limit of detection in seeded sputum samples was about 7 X 10(4) cells per ml for both monoclonal antibodies. Lung and tracheal lavage specimens from L. micdadei- or L. dumoffii-infected guinea pigs showed specific staining only with their respective monoclonal antibodies. The monoclonal antibodies stained homologous bacteria slightly less intensely than did the polyclonal antisera, but the signal-to-noise ratio was considerably higher for the monoclonal antibodies. No differences in sensitivity of staining of clinical specimens or bacterial isolates were noted between the monoclonal antibodies and the polyclonal reagents for L. micdadei and L. dumoffii (Centers for Disease Control, Atlanta, Ga., and BioDx, Denville, N.J. These monoclonal antibodies ae sensitive and specific, making them good candidates for laboratory diagnostic purposes.

Animals↗
PubMed1985

Retrospective analysis of two hundred and twelve cases of bacteremia due to anaerobic microorganisms.

Cases of bacteremia caused by anaerobic microorganisms and occurring during a four year period in a non-selected patient group in a Spanish general hospital were analysed retrospectively. Microbiological data was collected on 212 patients and clinical data on 103 patients. Cases of anaerobic bacteremia represented 8.6% of the total number of cases of bacteremia. Of the 232 anaerobic microorganisms causing bacteremia, gram-negative bacilli were responsible in 113 cases (48.7%), gram-positive bacilli in 92 cases (39.6%), gram-positive cocci in 25 cases (10.7%), and Veillonella spp. in two cases (0.8%). The most important clinical features were fever (64%), anemia (56%), septic shock (22%) and metastatic abscesses (21%). Patients with anaerobic bacteremia were hospitalized for an average of 51.7 days. The overall mortality was 32% and factors associated with poor prognosis were severe underlying disease, nosocomial acquisition, presence of shock, presence of metastatic foci of infection, and absence of adequate surgical drainage. The mortality rate of patients who received adequate antimicrobial therapy was 30% and that of patients who received inadequate treatment or none was 29%. It is concluded that anaerobic bacteremia has a significant rate of morbidity and mortality and that underlying disease and surgical debridement and/or drainage have greater prognostic significance than the use of antimicrobial agents.

Adolescent↗
PubMedDate not supplied

The CLED agar option in urine culture routine. A prospective and comparative evaluation.

Current recommendations for routine urine culture are still inoculation of specimens in a combination of solid media, usually blood agar (BA) plus MacConkey agar (MK). We have performed a prospective blind study comparing the accuracy of CLED agar (C), as the sole medium, with classic procedure (BA+MK). We included 1157 urine samples plated on the three culture media simultaneously. After incubation for 20-22 hr at 37 degrees C, the cultures were read by two of the authors independently. The cultures were evaluated according to standard criteria (BA+MK) considering a count above 10(4) CFU/ml as significant. The numbers of positive, negative, and contaminated cultures were 233, 764, and 160, respectively. Sensitivity and specificity of CLED were 98% and 99%, respectively. Fastidious microorganisms were accurately detected on C agar as well as on BA+MK. Morphologic differentiation of colonies was easier with C agar, and time required for inoculating and reading the plates was logically less in the C group. As the productivity of C is similar to that of standard procedures, its economy and convenience make it a medium worth using for routine culture of urine samples.

Agar↗
PubMedDate not supplied

Epidemiology of aerobactin production in Enterobacteriaceae.

Clinical isolates (496) of 10 different enterobacterial genera were studied for aerobactin excretion, colicin production and antibiotic resistance. In the case of Escherichia coli, the incidence of aerobactin-positive strains in 108 blood isolates (45%) was not significantly different from that corresponding to faecal sources (41%). Although colicin V production was much more frequently associated with aerobactin production than other colicins, colicin V was only produced by 26% of aerobactin excreters. Some strains were aerobactin-negative and colicin V producers. Aerobactin production seemed to be significantly associated with plasmidic antibiotic resistance. The production of the siderophore is described for the first time in Proteus, Serratia and Morganella strains.

Blood↗