A diaphragm cell and the procedure for studying isothermal diffusion in dental enamel.
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Biomedical subjects
Publications and source records attributed to E C Moreno.
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Diffusion fluxes of two anionic species through hydroxyapatite membranes were found to be reduced up to 50% by the presence of adsorbed salivary pellicles developed on the membrane surfaces. By contrast, water fluxes were only marginally affected, indicating that salivary pellicles display ionic permselectivity. This property is used to explain a remarkable protection of the enamel observed when salivary pellicles were developed on extracted teeth before exposure to acid lactate buffers.
Applications of APF and NaF solutions to extracted human teeth reduced the extent of enamel subsurface demineralization induced by colonization of S. mutans but were ineffective against demineralization by acid lactate buffers. It is concluded that the CaF2 formed on the tooth surface during the treatment is responsible for the protection observed.
Enamel subsurface demineralization induced by Streptococcus mutans was significantly reduced by seven-day saliva pre-treatments conducive to the formation of enamel pellicles. A two-hour saliva pre-treatment was ineffective. Results suggest that the protection provided by long-term pellicles may relate to changes in ionic transport rates rather than cell attachment.
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