A reliable method to detect bacterial contamination of blood components using an automated blood culture system.
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Bacteria↗
Biomedical subjects
E C Alport
Publications and source records attributed to E C Alport.
Clonal B cell populations in a minority of patients with Hashimoto's thyroiditis.
BACKGROUND: Hashimoto's thyroiditis (HT) is a risk factor for thyroid lymphoma, and clonal B cell populations in HT support this link. The literature on B cell clonality in HT is controversial. AIMS: To identify clonal B cell populations in HT and to assess their usefulness in differentiating HT from mucosa associated lymphoid tissue (MALT) lymphoma and predicting future development of lymphoma. METHODS: DNA from formalin fixed, paraffin wax embedded blocks of thyroid specimens from 10 patients with HT and two thyroid MALT lymphomas was analysed for B cell clonality by seminested polymerase chain reaction (PCR) using FRIII/LJH and FRIII/VLJH primers to amplify the IgH gene VDJ region. In one case, PCR products were sequenced. Immunohistochemistry was performed by labelled streptavidin-biotin technique using antibodies to: CD45, CD45RO, CD3, CD20, and cytokeratin. RESULTS: The histopathological and clinical findings were characteristic of HT. Clonal bands were seen in three and a polyclonal smear pattern was seen in seven cases. The clonal bands in HT were associated with a background smear, and could not be reproduced from other blocks from the same case or from deeper sections of the same block. The clonal bands in thyroid lymphomas were not associated with a background smear and were reproducible. None of the patients with clonal B cells has developed malignant lymphoma during a follow up of 10-13 years. CONCLUSIONS: B cell clonal bands in HT have different features from those in lymphoma (non-pure and non-reproducible) and do not predict future development of lymphoma.
Adult↗
Ifosfamide, doxorubicin and dacarbazine chemotherapy, and adjunctive laparoscopic cystoprostatectomy and external beam radiotherapy for prostatic embryonal rhabdomyosarcoma.
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Adult↗
Molecular analysis of clonality of sporadic angiomyolipoma.
Angiomyolipoma, which consists of three intimately intermixed components, smooth muscle, blood vessels, and adipose tissue, is variably considered a hamartoma, a choristoma or a true neoplasm. This study has investigated the clonality of sporadic angiomyolipomas in seven women, each with a single lesion, by determining the pattern of X-chromosome inactivation. Polymerase chain reaction (PCR) amplification of the highly polymorphic human androgen receptor gene (HUMARA) was performed on the DNA extracted from the paraffin-embedded lesional tissue microdissected to sample the admixed smooth muscle and blood vessel component (SMC/BV) and the adipose tissue component. All seven patients were heterozygous for HUMARA polymorphism upon amplification of undigested DNA from non-lesional tissue and were therefore informative for further analysis. In all patients, lesional DNA, representative of the components, was predigested with HpaII restriction enzyme for amplification of the methylated allele. In six patients, the lesions were clonal, while in one, polyclonal. The polyclonal lesion was small and had less than 20 per cent SMC/BV component. Microdissected SMC/BV component was clonal in 6/7 lesions; the scanty SMC/BV in the remaining lesion did not yield amplifiable DNA. Microdissected adipose tissue was polyclonal in all seven lesions. Angiomyolipomas are three clonal lesions due to a clonal smooth muscle cell and blood vessel component, while the polyclonal adipose tissue is probably metaplastic or reactive.
Actins↗
Fahr's disease associated with astrocytic proliferation and astrocytoma.
This report documents the neuropathological findings of astrocytic proliferation and astrocytoma in a patient with Fahr's disease. At autopsy, there was extensive bilateral symmetrical calcification involving basal ganglia, sulcal depth of the cerebral cortex, and dentate nuclei of the cerebellum. A large low-grade astrocytoma was identified in the left parietal lobe. Astrocytic proliferation was also noted in the areas of early calcification and at the margins of large calcareous deposits, away from the tumor.
Adult↗
Telangiectatic osteosarcoma: unusual behaviour.
The telangiectatic variant of osteogenic sarcoma is rare. Its biologic behaviour, treatment and prognosis are controversial. The case of a 15-year-old girl with this tumour is described. Both the location and clinical behaviour of the tumour were unusual. The tumour, which involved the distal ulna, was initially treated by a limited resection of the distal 8 cm of ulna. Sixty-nine months later the tumour recurred locally; there was no evidence of metastases. The forearm was amputated and the patient was then treated aggressively by chemotherapy. She was well 42 months later. This case illustrates the tendency for telangiectatic osteosarcoma to recur locally if it is not radically excised.
Adolescent↗
Pigmented villonodular synovitis of the temporomandibular joint.
Pigmented villonodular synovitis ( PVNS ) is a benign disease of unknown etiology arising from the synovial membranes of joints, bursae, and tendon sheaths. Though histologically benign, it is a very aggressive lesion, capable of bone destruction and widespread infiltration of surrounding tissues. It is a rare disease, usually confined to large joints, particularly the knee. Its propensity for recurrence is emphasized in this report. Involvement of the temporomandibular joint (TMJ) is unusual. It most commonly presents in the TMJ region as a left pre-auricular mass. Symptoms of TMJ dysfunction are usually present, but may be subtle. These may consist of painful mastication, clicking, trismus, malocclusion, and deep pain in the TMJ. Radiological evidence of bone destruction is frequently present, and together with the pre-auricular mass, should suggest the diagnosis. Surgical excision is the preferred mode of treatment. While cosmesis is a consideration, radical excision, including involved bone, is mandatory to prevent recurrence. The effect of radiotherapy on this disease in the head and neck region is documented.
Diagnosis, Differential↗
The syndrome of gastric argyrophil carcinoid tumors and nonantral gastric atrophy.
Records of the 30 cases of gastric carcinoid at the Mayo Clinic showed that the gastric mucosa was normal, hyperplastic, or atrophic (nonantral) in 12, 2, or 16 patients, respectively. In the atrophic group, the tumors were in the gastric body and fundus; small, polypoid, and multicentric; and associated with fundal argyrophil cell hyperplasia. In immunocytochemical studies, minor tumor cell populations stained positively for 5-hydroxytryptamine, gastrin, and somatostatin in 1 case and for 5-hydroxytryptamine in 3 others. Metastasis occurred in 3 patients. Twelve patients had pernicious anemia. Parietal cell or intrinsic factor antibodies or both were present in all 12 patients tested. Each of the 7 patients with an intact antrum had massive hypergastrinemia. No common HLA-A, -B, or -DR antigen pattern was detected among the 10 patients tested. The results suggest that nonantral gastric atrophy predisposes to gastric carcinoid as well as to gastric carcinoma.
Adult↗
An immunoenzyme assay for quantitation of human IgG antibodies to honeybee venom phospholipase A2.
A new method to measure the concentrations of IgG antibodies to phospholipase A2 in sera from patients treated with honeybee venom immunotherapy is described. This method utilizes a microcentrifugal analyzer to detect inhibition of PLA2 enzymatic activity by antibodies in serum standards and unknowns. Sera from beekeepers with known concentrations of specific antibodies, measured by radioimmunoprecipitation, were used to construct a logit-log standard curve for the immunoenzyme assay. The standard curve was linear for concentrations between 2.3 and 20.0 microgram/ml. The concentrations of specific antibodies measured by enzyme inhibition correlated well with the concentrations as measured by radioimmunoprecipitation, r = 0.959 (least squares linear regression), n = 32. Interassay variation was 10.1% at 10 microgram/ml. The immunoenzyme method is rapid and does not require radiolabeled reagents.
Antibodies↗