Quantitative determination of hemoglobin in urine. 1. The inhibitory effect of urine on the peroxidase-like activity of hemoglobin and on horseadish peroxidase.
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Alginates↗
Biomedical subjects
E C Adams
Publications and source records attributed to E C Adams.
Specific antisera to myoglobin.
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Animals↗
Studies on guinea pig oocytes. II. Histochemical observations on some phosphatases and lipid in developing and in atretic oocytes and follicles.
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Acid Phosphatase↗
Use of the capybara for antisera production.
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Amino Acid Sequence↗
Immunochemical confirmation of gastrointestinal bleeding.
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Animals↗
Differentiation of myoglobin and hemoglobin in biological fluids.
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Ammonium Sulfate↗
Differentiation of myoglobin and hemoglobin in biological fluids.
The use of several methods to differentiate myoglobin from hemoglobin has been investigated. The immunochemical methods, particularly those of hemagglutination inhibition and radioimmunoassay, are the most useful. This report summarizes work in the Ames Research Laboratory over the past 17 years with the several methods.
Hemagglutination Inhibition Tests↗
Radioimmunoassay for serum myoglobin.
A column flow-through radioimmune assay for serum myoglobin has been developed. The assay uses antibodies to human or rhesus monkey myoglobin coupled to Sepharose by cyanogen bromide activation and myoglobin labeled by the Chloramine-T method. The labeled myoglobin is stable over two to three half-lives. Serum myoglobin levels were elevated when serum was taken up to 12 hours after myocardial infarction and generally elevated up to 24 hours. A continued or repeated elevation of serum myoglobin probably indicates continued myocardial damage or new attacks. In the cases of myocardial infarction, levels up to 1340 ng per ml were found.
Animals↗