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Biomedical subjects

E Bottone

Publications and source records attributed to E Bottone.

At least 37 records · Page 2Linked to original sources
PubMed1971

Characteristics of a bacteriocin derived from Streptococcus faecalis var. zymogenes antagonistic to Diplococcus peumoniae.

A bacteriocin-producing strain of Streptococcus faecalis var. zymogenes (E-1) was isolated from clinical material (conjunctiva). The active substance differed from bacteriocins described by other investigators primarily in its spectrum of antibacterial activity, especially by its marked inhibition of Diplococcus pneumoniae. The E-1 bacteriocin also inhibited nonhemolytic strains of enterococci as well as one-third of the Viridans group of streptococcal strains investigated. The degree of inhibition, however, as indicated by the size of the zones against the latter organisms, was significantly reduced. No activity was detected against any of the strains belonging to the following groups of bacteria: hemolytic enterococci, beta-hemolytic streptococci, nonhemolytic streptococci, staphylococci, and various gram-negative species. Similarly, three strains each of Bacillus cereus and Listeria monocytogenes and one strain of Erysipelothrix insidiosa were not inhibited. The bacteriocin was able to diffuse through bacterial membranes as well as cellulose dialyzer tubing. It was inactivated by heating to 80 C for 20 min but resisted inactivation by either trypsin or chloroform.

Agar↗
PubMed1970

Unusual fermentative, gram-negative bacilli isolated from clinical specimens. I. Characterization of Erwinia strains of the "lathyri-herbicola group".

Five strains of gram-negative, yellow chromogenic bacilli were recovered from clinical specimens which fit the characteristics of the "lathyri-herbicola group" within the genus Erwinia. The strains were facultatively anaerobic, fermentative, anaerogenic bacilli with peritrichous flagella which grew at 37 C, reduced nitrate to nitrite, and failed to produce oxidase, pectinase, arginine dihydrolase, and decarboxylases for lysine and ornithine. Aggregations of bacteria (symplasmata) were observed in the syneresis water of slant cultures, and analogous granular aggregates and biconvex, spindle-shaped bodies developed in colonies on plate cultures. Awareness of these characteristics should result in more frequent identification of Erwinia species from human sources.

Adult↗
PubMed1970

Unusual fermentative, gram-negative bacilli isolated from clinical specimens. II. Characterization of Aeromonas species.

Morphological and physiological characterization of 12 Aeromonas strains isolated from clinical specimens demonstrated several important diagnostic features. These included polar arrangement of the flagella; production of oxidase, deoxyribonuclease, amylase, gelatinase, and lipase; lack of ornithine decarboxylase; and sensitivity to polymyxin. Awareness of these features should result in more frequent differentiation of aeromonads from the physiologically similar members of the genus Plesiomonas and the late- or non-lactose-fermenting species of Enterobacteriaceae.

Adult↗