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Biomedical subjects

E Bosisio

Publications and source records attributed to E Bosisio.

At least 55 records · Page 3Linked to original sources

Pulmonary diffusing capacity and its components (DM and Vc) in young, healthy smokers.

We have studied the diffusing capacity and its components (DM and Vc) in two groups of 9 males, healthy, smokers and nonsmokers, with similar age, biometric characteristics and spirographic values. Also, BT was not different between the two groups, because the smokers were asked to stop smoking 12 h before the test. Our data showed that TF and DM are significantly lower in smokers than in nonsmokers (p less than 0.005 and less than 0.01, respectively, while Vc was not different between the two groups and confirm that Vc is influenced by COhb level.

Adult↗

Ventilatory and pressor response to isometric exercise in normal subjects.

We studied in 8 young, healthy males, the behaviour of heart rate (HR), systolic blood pressure at the upper (SBPUL) and lower (SBPLL) resting limbs, pulmonary ventilation (VE), oxygen uptake (VO2) and production of CO2 (VCO2) during and after two levels of isometric effort (handgrip at 50 and 100% maximum voluntary contraction; MVC). HR, SBPLL, VE, VO2, and VCO2 did not vary between the two efforts, but SBPUL is significantly different between them. The possible mechanism and predominant role of nervous reflexes upon the pressor and ventilatory response to isometric exercise are discussed.

Adult↗

[Effects of handgrip on cardiovascular and respiratory apparatus of healthy young subjects (author's transl)].

We have studied, in eight healthy subjects, aged 22-26 years, some cardiovascular and respiratory effects, during maximal and half-maximal isometric effort, four minutes lasting, performed with hand (handgrip). During contraction and 15 minutes lasting recovery, heart rate, arterial systolic pressure at resting arm and at limb, pulmonary ventilation, VO2 and VCO2 have been evaluated. The data of the two tests have been submitted to the Student's test. During isometric contraction and recovery, heart rate rapidly increases and decreases, respectively, without significant differences between the two contractions. The abrupt increase of the systolic arterial pressure is greater during maximal work, at resting arm; arterial pressure at limb increases rapidly during contraction, but do not varies between the two efforts. These arterial pressure responses to isometric contraction presumably are related to a neurogenic vasoconstriction. During recovery of maximal work, the systolic arterial pressure, at limb, is significantly greater than basal values from 5th to 15th minute and versus the half maximal effort at 7th, 10th and 15th minute, presumably because the vasoconstriction is still effective. Pulmonary ventilation, VO2 and VCO2 increase during contraction; VO2 and VCO2 further increase after 10 minutes of recovery, probably because the debt of O2 is repaid.

Adult↗

Respiratory response to carbon dioxide after propranolol in normal subjects.

The ventilatory response to carbon dioxide, using the rebreathing technique, was investigated in 5 healthy nonsmoker volunteers, without obstructive bronchopathy. The administration of propranolol (20 mg) in a single oral dose did not produce significant modifications of the slopes of the response curves, but caused a significant increase of the intercept of the curves (p less than 0.05). Since no changes of the spirographic values were noted, the results obtained were attributed to a decrease of ventilation. It is concluded that propranolol, even at the dose of 20 mg, is able to induce a depression of the respiratory center, concomitant with significant reduction of heart rate and arterial blood pressure.

Adult↗

[Effort test on a treadmill in peripheral arteriopathic obstructions (author's transl)].

20 males, 48 to 66 of age, whose 10 normal and 10 with chronic arteriopatic obstruction at inferior limbs, were submitted to effort on a treadmill for 5 minutes or until pain. The clinostatic systolic aterial pressure was obtained in the four limbs before and at fixed intervals of time from the exercise. Arterial pressure increases after effort in the normal limbs; the length and the amount of the systolic pressure decrease after exercise are in correlation with the degree of the arterial involvement. The exercise on treadmill seems useful when the clinical and other diagnostic data are inadequate for a reliable diagnosis and to evaluate the progression of the arteriopathy through a non-invasive, and easy to repeat, method. The possible mechanisms of the pressor decrease in the arteriopatic limbs are discussed.

Aged↗

Relations between prostaglandin E2, F2alpha, and cyclic nucleotides levels in rat brain and induction of convulsions.

Fully convulsant doses of pentamethylenetetrazole cause marked increase in rat brain cortical PGF2alpha, PGE2, cGMP and cAMP during seizures, whereas subconvulsant doses cause an increase of rat brain cortical PGF2alpha without affecting the other biochemical parameters considered. Rat cerebellar prostaglandins were not modified by the convulsant agent at either dosage.

Animals↗

Catabolism of cholesterol by bovine adrenal-cortex enzymes: in vitro formation of oxygenated sterols and side-chain cleavage products.

The identification of sterols and steroids formed by incubation of [7alph-3H, 26-14C]cholesterol with mitochondrial enzymes from bovine adrenal cortex is reported. Only 17% of the radioactivity associated with cholesterol metabolites was found in pregnenolone, whereas 15% was reliable to oxygenated sterols and 6% to steroid compounds. The significance of the formation of these compounds is discussed particularly as regards oxygenated cholesterol derivatives.

Adrenal Cortex↗

Correlation between release of free arachidonic acid and prostaglandin formation in brain cortex and cerebellum.

Levels of free arachidonic acid and of prostaglandin F2alpha and E2 have been measured in both brain cortex and cerebellum of rats killed by focussed microwave irradiation, and after decapitation followed by ischemia. The same parameters were studied during incubation assays. It was found that: a) after ischemia levels of both free arachidonic acid and of prostaglandins in cerebellum are lower than in brain cortex, b) formation of prostaglandins from endogenous precursor in incubated cortex is higher than in cerebellum, c) release of free arachidonic acid occurs mainly during the time interval between the sacrifice of the animals and the beginning of the incubation, whereas prostaglandins are formed mainly during the incubation assay. The correlation between release of free arachidonic acid and prostaglandin formation is discussed.

Animals↗