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Biomedical subjects

E Berti

Publications and source records attributed to E Berti.

At least 127 records · Page 7Linked to original sources
PubMed1987

Generalized eruptive histiocytoma in children.

Generalized eruptive histiocytoma is a papular, non-X, nonlipidic, self-healing histiocytosis affecting mainly adults. This article describes the clinical, histologic, ultrastructural, and immunologic findings in four children, aged 10 months to 4 years, who were suffering from this disease. Generalized eruptive histiocytoma of children differs from generalized eruptive histiocytoma of adults only in that the lesions are not symmetrically distributed, they do not affect the mucous membranes, and they may become xanthomatous.

Biopsy↗
PubMed1986

Angiosarcomas in lymphedematous limbs.

The clinical and histopathological features of four cases of angiosarcoma in congenital, post-traumatic, and post-hysterectomy lymphedematous limbs are reported. A good correlation between histologic and ultrastructural findings and clinical course was observed.

Cell Transformation, Neoplastic↗
PubMed1986

Normolipemic eruptive cutaneous xanthomatosis.

We describe a form of diffuse, rapidly self-healing xanthomatosis with the clinical and ultrastructural features of hyperlipemic xanthoma but occurring in a subject with normal lipid metabolism and with no associated systemic disorders.

Adult↗
PubMed1985

In situ identification of immune competent cells in gastrointestinal mucosa: an evaluation by immunoelectronmicroscopy.

The in situ identification of lymphocyte subpopulations by means of immunopathological techniques using specific monoclonal antibodies provides a tool for the study of the gastrointestinal-associated lymphoid tissue (GALT) in health and disease. In this field, monoclonal antibodies have been applied previously using light microscopy and either immunofluorescence or immunoperoxidase; however, these techniques are not sensitive enough to allow precise evaluation of localization of labelling. We describe an immunoelectronmicroscopic method, which defines labelling specificity, since it allows the identification of cells by immunophenotype labelling and ultrastructural markers simultaneously. This in turn allows a better evaluation of the labelled cells and of the relationship between labelled and unlabelled cells. The main features of the method are the use of fresh tissue samples, fixing in paraformaldehyde CaCl2, and the coupling of the immune reaction to an amplification system (avidin-biotin-peroxidase complex). The technique yields a good preservation of cellular ultrastructure, together with a strong and specific immunolabelling. Our results confirm the high specificity of monoclonal antibodies when applied to immunopathology techniques. We confirm the pattern of distribution of various lymphocyte subsets in the jejunal mucosa described by other authors by light microscopy.

Antibodies, Monoclonal↗
PubMed1985

Functional and molecular characterization by the CB04 monoclonal antibody of a cell surface structure exerting C3-complement receptor activity.

CB04 monoclonal antibody which reacts with an epitope of a surface molecule expressed on human monocytes has been elicited using peripheral blood lymphocytes as immunizer. The characterization of the monoclonal antibody examined at the phenotypic, molecular, and functional levels indicates that the CB04 antibody defines a structure present on monocytes, tissue macrophages, B cells, polymorphonucleates, and erythrocytes. The molecular weight (220 kD), the tissue distribution in health and disease conditions, and the involvement in relevant biological processes indicate that the CB04 structure is the receptor for the C3b fragment of the complement. The binding of the antibody to the cell surface induces inhibition of the C3bi receptorial function.

Animals↗
PubMed1985

Heterogeneity of dermal OKT6+ cells in inflammatory and neoplastic skin diseases.

This immunopathologic study of both normal and pathologic skin specimens (contact dermatitis [CD], lichen planus [LP], cutaneous T cells lymphoma [CTCL], and histiocytosis X [HX]) allowed as to differentiate four types of dermal OKT6+ cells: (1) cells with the same morphologic features as epidermal Langerhans cells (LCs), rarely found in either normal or pathologic dermis; (2) cells structurally similar to LCs but lacking Birbeck granules (BGs), found mainly in CD and LP; (3) larger cells rich in cytoplasmic organelles, only 5% of which contained BGs. They were especially common CTCL; and (4) cells typical of HX.

Adult↗
PubMed1985

Immunohistochemical reactivity of anti-LAV p18 monoclonal antibody in lymph nodes from PGL and AIDS patients.

This study deals with the immunohistochemistry of a monoclonal antibody (Mab) raised against the p18 protein of the LAV virus in lymphnodes from 20 cases of persistent generalized lymphoadenopathy (PGL) (2) and 6 of acquired immunodeficiency syndrome (AIDS) (3). In all the PGL cases that have been studied, we observed a very important disruption of the follicular dendritic cell's (FDC) framework in the germinal centers which is associated with the presence of an increased number of Leu2a+ lymphocytes inside the germinal centers. This observation is consistent with the main morphological feature of the lymphnodes in the PGL syndrome which is mainly characterized by lesions of FD cells.

Acquired Immunodeficiency Syndrome↗
PubMed1984

Letterer-Siwe disease in an octogenarian.

The case of an 81-year-old woman suffering for about 1 year from a typical form of Letterer-Siwe disease (LSD) is reported. The only important systemic involvement was hepatosplenomegaly. The patient was treated with steroids and vinblastine and then with thymopoietin pentapeptide. This last drug proved useful for decreasing the dose of steroid and thus for checking the appearance of new skin lesions. The ultrastructural investigations, carried out before and after vinblastine, showed the presence in some histiocytosis X cells (HXC) of coated Birbeck granules (BG). Immunoelectron microscopy showed about 90% of HXC to be OKT6+ and the OKT6 labeling of these cells to be similar to that of the Langerhans cells of normal skin. Five percent of OKT6+ cells apparently contained no Langerhans granules.

Age Factors↗
PubMed1983

The avidin-biotin peroxidase complex (ABCPx)/in skin immunoelectron microscopy.

The interaction between avidin and biotin can be exploited in immunoperoxidase techniques in three main way: the labeled avidin-biotin technique (LAB); the bridge avidin-biotin technique (BRAB); the performed avidin-biotin complex (ABCPx). In a comparative study of these three methods and the peroxidase antiperoxidase method using serial dilutions of antinuclear positive serum, the ABCPx method yielded the most intense staining. For this reason, the ABCPx method has been applied in immunoelectron microscopy (IEM) techniques on normal and pathological skin employing monoclonal OKT4, OKT6, OKT8 antibodies to detect target membrane antigens. The authors notes that the ABCPx method allows strong staining both in normal and pathological skin and improves tissue preservation.

Antigens↗
PubMed1983

Small-bowel involvement in dermatitis herpetiformis and in linear-IgA bullous dermatosis.

In 23 patients with dermatitis herpetiformis (DH) and five patients with linear-IgA bullous dermatosis (BD), we evaluated the occurrence of histologic jejunal changes and small-bowel function abnormalities. None of the patients showed clinical signs or symptoms of malabsorption. Morphological jejunal changes consistent with gluten-sensitive enteropathy were found in 82% of DH patients and in 60% of BD patients. However, BD patients showed only mild jejunal histologic abnormalities, whereas more severe jejunal lesions were found in most patients with DH. Functional tests showed a rough correlation with the severity of the jejunal lesions, being almost completely normal in BD patients and DH patients with mild intestinal damage, whereas most of DH patients with subtotal or total villous atrophy showed abnormal d-xylose tests and folic acid assays. Lactose tolerance tests (H2 breath test and blood glucose after oral lactose load) showed no correlation with the degree of jejunal damage.

Adult↗
PubMed1983

The morphologic events of blister formation in porphyria cutanea tarda.

In porphyria cutanea tarda (PCT), the submicroscopic event causing the formation of spontaneous blisters is the appearance of membrane-limited vacuoles in the superficial dermis. These vacuoles are apparently formed through cytolysis of dermal cells and swelling of the pseudopodia of the basal cells protruding into the dermis, probably as a consequence of photochemically-induced lysosomal damage. The number and size of these vacuoles progressively increase near the cleavage area of the blisters, where their limiting membranes merge and break, giving rise to the cavity of the blister, which is formed under the basal lamina. The presence of coated vesicles along the limiting membranes as well as inside the vacuoles suggests their involvement in the transport of fluids from the damaged vessels, thus contributing to the progressive volume increase of vacuoles themselves.

Adult↗
PubMed1981

Collagen phagocytosis in multicentric reticulohistiocytosis.

A large number of dermal cells engaged in collagen phagocytosis were observed in a case of multicentric reticulohistiocytosis. These cells apparently start to engulf collagen by deep invaginations of the plasma membrane. Fusion of phagosomes containing the engulfed collagen with vesicles probably originating from the Golgi apparatus would give rise to secondary lysosomes. The process of collagen degradation leads to the appearance of fine fibrils and myelin bodies. The presence in the dermis of unusually thick collagen fibers might contribute to this process which appears to occur only under particular physiological and pathological conditions.

Cell Membrane↗