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Biomedical subjects

E Bendvold

Publications and source records attributed to E Bendvold.

28 records · Page 2Linked to original sources
PubMed1985

On the origin of prostaglandins in human seminal fluid.

To evaluate the origin of seminal prostaglandins, genital tract fluids were analysed for prostaglandin content in two clinical situations. Six patients delivered semen samples before and after vasectomy. In these patients the prostaglandin concentration remained essentially unchanged although sperm density decreased to zero. In another patient secretory products from the testis and epididymis, and the ejaculate representing mainly the secretion of the seminal vesicles and the prostate gland, were collected separately. Secretions obtained from the testis and epididymis did not contain detectable amounts of prostaglandins, whilst in the ejaculate from the same patient the concentration was within normal limits. The results of the study show that the testis and epididymis do not contribute significantly to the prostaglandin content of human seminal fluid. The previous assumption that the seminal vesicles are the main source of seminal prostaglandins is thus supported.

Epididymis↗
PubMed1984

The natural variations in prostaglandin concentration in human seminal fluid and its relation to sperm quality.

Prostaglandins E, F, 19-hydroxy E, and 19-hydroxy F (PGE, PGF, 19-OH-PGE, and 19-OH-PGF) in human semen were determined by a new chemical method. The method allows liquefaction to occur, a prerequisite for simultaneous estimation of PG concentration and sperm characteristics in the same ejaculate. The present study showed also that the variation in PG concentration between different individuals was more pronounced than in repeated ejaculates from the same individual. Sperm density and sperm motility seemed related to PG concentration. A high density was associated with a low PG concentration. The concentration of 19-OH-PGE was higher and that of 19-OH-PGF lower in ejaculates with a normal sperm motility than in those with an abnormal motility. It seems that functional requisites of sperm depend upon optimal seminal PG concentration.

Alprostadil↗
PubMed1982

Quantification of prostaglandins in human seminal fluid.

A method is described for measurement of the prostaglandins present in human seminal plasma. The PGEs and the 19-hydroxy-PGEs are converted to the respective PGB-compounds. They are determined with UV-absorbance after purification with two chromatography steps. The PGFs and the 19-hydroxy-PGFs are determined by gas chromatography, which also allows measurement of the 8 beta-isomers. Recovery of added PG was in the average 99.2% (range 89.9-107.7). Mean variation between duplicate analyses was 5.6% (range 0.9-9.1). The method has been simplified to allow at least limited clinical use.

Chromatography, Gas↗
PubMedDate not supplied

The CellSoft computerized semen analysis system. I. Consistency of measurements and stability of results in relation to sample size analyzed.

The CellSoft TM computer-assisted digital image analysis system was evaluated for reproducibility of measurements and for the sample size needed to obtain stable results for seven parameters characterizing human spermatozoa. The parameters were sperm density, velocity, linearity, percent motile cells, maximum and mean amplitude of sperm head's lateral displacement (ALH max and ALH mean) and beat cross frequency. Thirty men were assigned to three equally large groups according to sperm density, and the groups were studied separately. Consistency of measurements was highly acceptable for all parameters (coefficient of variation ranging from 0.9% to 3.7%). The average minimum number of sample size needed for stable results varied between the groups and within each group dependent upon the parameter studied. Velocity and linearity seemed to require the lowest average cell number, while percentage motile cells and ALH max needed the highest sample size. Our current recommendation of sample sizes is outlined in detail and limitations and future utility of CellSoft is discussed.

Evaluation Studies as Topic↗
PubMedDate not supplied

Depressed semen quality in Swedish men from barren couples: a study over three decades.

The semen quality of men who participated in an infertility investigation in 1956 was compared with that from men from similar populations in the years 1966, 1976, and 1986. The sperm parameters studied included total sperm count and sperm morphology. A significant reduction over this 30-year period was observed for both parameters. The total sperm count decreased from 467 million in 1956 to 305 million in 1986 (mean, p less than 0.0001) and the percentage of sperm with normal morphology diminished from 53% to 37% (mean, p less than 0.0001) during the same period. This Swedish study, like studies in other industrialized countries, indicates a decline in semen quality over the past few decades.

Aging↗
PubMedDate not supplied

The CellSoft computerized semen analysis system. II. On the optimalization of running conditions concerning gray scale and cell size range.

The CellSoft automated semen analysis system was studied concerning the effect of gray scale (GSc) variation on cell motion parameters and to establish an appropriate setting for human spermatozoal sizes (PixMin and PixMax) based on the cell's instant velocities (InsVels). GSc could be set within a range from "optimum" till +10 units without, except for curvilinear velocity, the CellSoft generated sperm measures being altered (p greater than 0.05). The aspect of a sample specific GSc setting according to certain criteria is stressed. Considering the area of immobilized spermatozoa (at GSc + 20) and of motile cells with low InsVel, PixMin should be set at 3 pixels. Correspondingly, the InsVel determined setting for PixMax (at a maximum velocity threshold set at 200 microns/sec.) was 28 pixels. A final recommendation on the setting for cell sizes can, however, only be determined in considering sizes of nonsperm seminal elements and in studying the appropriate setting for the maximum velocity threshold.

Computer Systems↗
PubMedDate not supplied

Semen quality in Norwegian men over a 20-year period.

Semen parameters of 135 randomly selected men in 1966 were compared with semen analysis of 148 men in 1986 to assess whether semen characteristics had changed during the period. Both groups of men were of barren couples; median age was identical in the two groups (31 years). The semen analyses included volume, sperm density, and sperm morphology. Significant change over the time period was observed only for the percentage of normal morphology. This parameter deteriorated from 60% in 1966 to 41% in 1986 (median values; p less than .001). This study, like studies from other industrialized countries, indicates a decline in semen quality over the past decades.

Adult↗