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Biomedical subjects

E Bender

Publications and source records attributed to E Bender.

At least 55 records · Page 3Linked to original sources
PubMed1983

Model conformations of the carboxyl telopeptides in vivo based on type I collagen fibral banding patterns.

Densitometric scans of high resolution banding patterns of uranyl acetate-stained, type I collagen fibrils teased from rat tail tendon were correlated with sequence derived model scans. The best model for the alpha 1 carboxyl telopeptide has the first 15 residues in a "S fold" or "random coil" with a net axial displacement of 7 residues (a contracted region) and the last 10 residues extended along the fibril axis. The correlation of both models with experimental scans is significantly improved when two 4D-staggered intermolecular crosslinks are modeled by eliminating four charges in band a3: the two lysines in the alpha 1 carboxyl telopeptides and two hydroxylysines at residues 87 and 88 in the triple helix. The alpha 2 carboxyl telopeptide is modeled as being folded back on the triple helix. Also, as previously found for type I segment long spacing crystallites, the highest correlation of models with the experimental data is obtained when the alpha 2 chain is in the A or reference position of the triple helix (alpha 2, alpha 1, alpha 1). It is further hypothesized that unfolding of the "S fold" or "random coil" in the alpha 1 carboxyl telopeptide may be a reversible mechanism for low strain energy dissipation in tendon and other connective tissues.

Animals↗
PubMed1983

[In vitro cultivation of vital tissue slices: a new variation of organ culture technics].

A new modification of the organ culture technique is described. In contrast to the conventional technique in which organ pieces (1 mm3) are cultivated, uniform, viable, microscopically controlled tissue slices are used in a modified organ culture technique. The new method is suitable for in vitro culture of human mammary carcinoma. Flow cytometry measurements reveal changes prior to and after collagenase treatment of human mammary carcinomas. In organ culture the composition of cell population prior to and after 48 h could be maintained. Examination under the microscope confirmed these results. The uptake of [3H]-thymidine was greater in tumor slices than in tumor pieces. Organ cultures sufficiently represent carcinoma in vivo in comparison to the other mentioned in vitro techniques.

Breast Neoplasms↗
PubMed1983

[Pectoralis muscles].

Origins, length, and insertion zones of the pectoralis muscles are described. The pocket of the pectoralis major and the square area of these muscles and some variations (Mm. sternales and the muscular arch) are included.

Adult↗
PubMed1982

Type I collagen segment long spacing banding patterns. Evidence that the alpha 2 chain is in the reference or A position.

Densitometric scans of electron micrographs of type I collagen segment long spacing crystallites stained with uranyl acetate or phosphotungstic acid and uranyl acetate have been correlated with computer-synthesized scans derived from the sequence of the alpha 1(I) and alpha 2 chains. Three models that differ in the location of the alpha 2 chain were used in the computer synthesis; Models A, B, and C have the alpha 2 chain in the A, B, and C chain positions, respectively. For all 13 experimental scans, the order of decreasing correlation with the models was found to be A,B,C. The probability of getting the same order of decreasing correlation all 13 times is 6/6(13). It was also determined at the 0.99 confidence level that the mean of the differences in the correlation coefficients among the models is greater than 0, supporting the conclusion that sequence-derived models best fit the experimental data when the alpha 2 chain is in the A position. Our results also agree with recent studies that show that uranyl ions bind to both positively and negatively charged residues on collagen type I.

Animals↗
PubMed1980

The protein pattern of PMF virus, a type-D retrovirus from malignant permanent human cell lines.

The protein pattern of a type-D retrovirus (PMFV) isolated from and propagated in human cell lines has been investigated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Staining with Coomassie blue and labeling with 14C-leucine/14C-lysine revealed five viral polypeptides with molecular weights of 10,000, 12,000, 15,000, 25,000, and 68,000. The 68,000 D-protein was shown to be a glycoprotein by incorporation of 3H-glucosamine and the 15,000 D-protein was identified as a phosphoprotein. By comparing PMFV with the closely related Mason-Pfizer monkey virus (MPMV) in co-electrophoresis experiments no clear difference was detected in viral 14C-leucine/14C-lysine profiles. The viruses differ, however, with respect to their glycoprotein patterns. A glycoprotein corresponding to the gp20 of MPMV has not been detected in PMFV irrespective of the cell line used for propagation of viruses.

Cell Line↗
PubMed1976

[Morphological studies on cellfree induced sarcomas in syrian hamster (author's transl)].

Morphological studies on sarcomas induced in syrian hamsters by cellfree transmission are described. The tumour tissue for the cellfree preparations stemmed from a sarcoma, containing C-particles. Basically, three histological groups have been distinguished: 1. neoplasms of the peripheral nerve-sheath, 2. undifferentiated sarcomas, and 3. liposarcomas. Furthermore, a rhabdomyosarcoma, an angiosarcoma and, in a heterotransfection on rat, an osteosarcoma have been established. The great majority of tumours could be transmitted by cellfree preparations. To this neoplasms belong the undifferentiated histological structure.

Animals↗
PubMed1976

[Sarcomas in syrian hamster, induced by an oncorna virus and cellfree transmissible (author's transl)].

1. Application of hamster papova virus to newborns of Syrian hamster has produced some s.c. sarcomas after a 5 to 6 month latency period, by virtue of the strong inducer effect of this papova virus to endogenous (latent) oncorna viruses. 2. Cellfree filtrates from a polymorphorus-cell sarcoma produced in this way, when applied to newborn hamsters of the spontaneously tumour-free hamster line HaP, again lead to sarcoma formation after a latency period of 3--8 months in about 20% of the animals; the same holds for cellfree filtrates of these cellfree induced sarcomas and their transplantation generations. 3. In these tumours C-type oncorna viruses, but no papova virus, could be demonstrated regularly. 4. The hamster specificity of this sarcoma virus is suggested by the complete absence of a tumorigenic effect of the cellfree filtrates from these hamster sarcomas in mice and rats. The preferential induction of hamster sarcomas by sarcoma filtrates, in conjunction with the fact that filtrates from hamster leukoses, indicates a certain difference between hamster leukemia and hamster sarcoma viruses.

Animals↗
PubMed1976

[Mesothelioma in syrian hamster. V. Macroscopic and histologic investigation of transplanted mesothelioma in solid and ascitic form (author's transl)].

Transplantable mesothelioma lines are described, which have been inoculated successively either s.c. or i.p. in solid and ascitic form. Increases of the degree of malignancy could be demonstrated for macroscopically biological parameters. The latent periods became shorter with increasing in vivo passages. In the histological picture, progressive dedifferentiation of the tumour cells with rising tendency towards infiltrative growth was observed.

Abdominal Neoplasms↗
PubMed1976

[Elimination of a paramyxo-type virus from oncornavirus-producing human cell lines (author's transl)].

A virus of the paramyxo-type was eliminated from cell-free material of human oncornavirus-producing cell lines (PMF). After transmission of this paramyxovirus-free inoculum to a human permanent cell strain (Tu 197/Tr 1) oncornaviruses were permanently formed and no paramyxoviruses could be detected. The paramyxovirus-free, oncornavirus-producing PMF-39 cell line could be established after inoculation of the TU 197/Tr 1 line with cell-free material containing both oncorna- and paramyxovirus diluted 1 to 1000. A second way of elimination of the paramyxovirus was the treatment of cell-free material containing both viruses with antisera against paramyxovirus. In the Tu 197/Tr 1 line inoculated with such material only oncornaviruses were formed. The second paramyxovirus-free oncornavirus-producing cell line was designated PMF 50.

Animals↗
PubMed1976

[Investigation of electrophoretical enzyme phänotypes glucose-6-phosphate dehydrogenase, red cell acid phosphatase, phosphoglucomutase and adenosine deaminase of permanent human cell lines (author's transl)].

Isoenzymes of 11 cell lines were investigated by electrophoretical separation. All lines have been cultivated from tissues of white persons, all but one (Leuc. Th. B.) were phosphoglucomutase 1 and all were adenosine deaminase 1. Three out of 11 cell lines did show glucose-6-phosphate dehydrogenase (G6PD) type B as expected. Two out of 8 cell lines with G6PD A were distinguishable by their specific type of "red cell" acid phosphatase (SEP). We conclude that in vitro the electrophoretical G6PD phänotyp B changed to phänotype A. Further 4 lines had other peculiarities which are indicative to their originality, though they were G6PD A. Our investigations did show that G6PD may become type A if a cell line changes to permanent growth capacity in vitro. The enzyme marker G6PD A alone may not be valuated as an absolute evidence for contamination or mix up with He-La Cells.

Acid Phosphatase↗
PubMed1975

[Study on transplantable, melanin containing hamster papova virusinduced skin tumours in Syrian hamster (author's transl)].

A melanin containing skin tumour of trichoepitheliomatic character, induced by a virus some 40 nm large, is described showing neither infiltrative nor metastasizing growth. The multiple, flat, mostly confluent tumour nodes appear in all parts of the skin, especially in dorsal skin, at the chin, eyes, and ears. The tumours are mostly blue black as a result of hemosiderin and melanin deposits; these pigmentations occur in connective-tissue cells, the tumour cells and the abounding keratinizing cysts. The blastomic character of these tumours, which in part are reminiscent of tricho-epitheliomas, can be demonstrated by transplantation experiments, in which with increasing number of passages are observed a decrease of latent period, disappearance of pigment formation, and increase in histologic de-differentiation.

Animals↗
PubMed1975

[A transplantable myeloid hamster leukemia with high peripheral leukocyte counts and C-particles. I. Transplantation experiments, pathogenesis, and electron-microscopic observations (author's transl)].

In this article is reported a generalized leukemia of the myeloproliferative system of golden hamsters capable of cellular transmission. Cellfree transmissions to 83 golden hamsters after a 6-month latency period, have so far yielded the same result in three cases. After cellular transplantation leukocyte counts of 180,000 on the average occured abruptly in the end-phase of the disease. Latencies were between 4 and 10 days. Electronmicroscopically, oncorna-viruses were demonstrated in hamsters inoculated with either the cellular or the cellfree preparation that bear close resemblance to the murine C-particles.

Animals↗
PubMed1975

[A transplantable myeloid hamster leukemia with high peripheral leukocyte counts and C-particles. II. Histology and cytochemistry (author's transl)].

Results on morphologic and hematologic characterization of a hamster leukemia capable of both cellular and cellfree transmission are described. Solid tumors removed in the course of several passages of leukemia animals, after producing blood smears, and spleen, liver, lymph nodes, bone marrow, thymus, kidney and lung were investigated histologically and histochemically. The morphological picture of the hamster leukemia has not changed during several transplantation generations. In addition to solid tumours, typical leukemic infiltration were detected histologically in spleen, liver, lymph nodes, bone marrow, kidneys and lung. No leukemic proliferation was noticed in the thymus. The final stage of the disease is characterized by an abrupt occurrence of high leukemic cell counts. The demonstration of alkaline phosphatase, but especially of naphtol-AS-D-chloroacetate esterase, in the leukemic cells is interpreted as indicating malignization of cells of the granulocytic line.

Alkaline Phosphatase↗
PubMed1975

Serological analysis of an oncornavirus (PMF virus) detected in malignant permanent human cell lines.

Immunodiffusion analysis of the PMF virus which was detected in malignant permanent human cell lines revealed positive reactions with antisera against the Mason-Pfizer monkey virus (MPMV). No cross-reactivity was demonstrated with murine leukemia virus (MuLV), rat leukemia virus (RaLV), hamster leukemia virus (HaLV), feline leukemia virus (FeLV), simian (woolly monkey) sarcoma virus (SSV-1) and mouse mammary tumor virus (MTV). The cross-reactive antigens of the PMF virus and the MPMV are considered as evidence for the human origin of the PMF virus.

Animals↗