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E Bell

Publications and source records attributed to E Bell.

154 records · Page 9Linked to original sources
PubMedDate not supplied

Prolylhydroxylase activation in IMR 90 fibroblasts: state of differentiation rather than population doubling level determines requirements for enzyme activation.

The stimulation of prolylhydroxylase activity by ascorbate in IMR 90 fibroblasts grown as monolayer decreases with increasing population doubling level, but the enzyme is not stimulated in IMR 90 fibroblasts of either low or high population doubling level incorporated into collagen lattices in which they cease to divide. The capacity to be activated is restored when cells are released from the lattice. We consider that reduction of stimulation of prolylhydroxylase activity by ascorbate as the population doubling level of cells grown as monolayer increases is not a phenomenon of cell aging. Rather, it is due to an increase in the size of the noncycling subpopulation whose state of differentiation is not the same as that of cells in cycle.

Cell Differentiation↗
PubMedDate not supplied

A new material for splinting neonatal limb deformities.

We report the use of Permagum, a silicone rubber dental-impression material, in the management of neonatal limb deformities. A wide range of custom-made splints can be quickly and easily made from Permagum for both the upper and lower limb. The material is not sufficiently rigid for the older, heavier child but is particularly useful in the neurologically impaired child.

Foot Deformities, Congenital↗
PubMedDate not supplied

Association between myotonic dystrophy and primary hyperparathyroidism.

A case of primary hyperparathyroidism in a patient with myotonic dystrophy is reported. A 56-year old female with myotonic dystrophy, admitted to hospital with a urinary tract infection, had widespread muscle atrophy and myotonia with bilateral cataracts. Biochemical findings of normal renal function but raised blood calcium, depressed blood phosphate and increased parathyroid hormone, were consistent with a diagnosis of primary hyperparathyroidism. Thallium scanning of the parathyroids showed an area of discordant thallium suggesting a parathyroid adenoma. When the left lower parathyroid was later excised, histology was consistent with the diagnosis of parathyroid adenoma. As far as the authors are aware this is the first report of myotonic dystrophy and primary hyperparathyroidism in the same patient.

Adenoma↗
PubMedDate not supplied

Growing straight.

Explore the source record for details and available documents.

Arm↗
PubMedDate not supplied

Sporicidal testing of commercial germicides using a chemical standard and a calibrated bioindicator.

The AOAC sporicidal method uses as a standard the resistance of spores on carriers to 2.5N HCl. This resistance is variable at exposure times ranging from 2 to 20 min. The method described in this paper uses a glutaraldehyde standard and distinguishes various levels of sporicidal activity in the presence of 1-5% glutaraldehyde by using appropriate spore strains, spore preparations, and spore levels. The resistances of 2 Bacillus subtilis 19659 spore preparations cultured in 10% Columbia broth plus manganese and nutrient agar plus minerals, as well as that of B. subtilis var. niger cultured on Lab-Lemco agar, were tested. T-soy broth was a better recovery medium than fluid thioglycollate or modified fluid thioglycollate for B. subtilis 19659 spores exposed to HCl. Sporicidal tests were done on B. subtilis 19659 spores with 2 types of spore preparations. A commercial glutaraldehyde germicide was used for comparison of the sporicidal activity of the glutaraldehyde standard. Two strains of B. subtilis spores and 4 levels of spores (20,000-80,000, 100,000-400,000, 500,000-800,000, and 1,000,000 and up) were removed from check penicylinders from the same batches used for sporicidal tests. B. subtilis var. niger spores were the most resistant to HCl, while B. subtilis 19659 spores were more resistant to glutaraldehyde. Sporicidal activities of a commercial germicide containing 2.5% glutaraldehyde with additives and another containing 5% glutaraldehyde in phosphate buffer were similar. Both totally destroyed high levels of B. subtilis 19659 spores cultured in 10% Columbia broth plus manganese. Results indicate that use of a glutaraldehyde standard, calibrated numbers of spores on penicylinders (bioindicators), and appropriate spore strains and preparations can reduce the variability of sporicidal testing of commercial germicides.

Bacillus subtilis↗
PubMedDate not supplied

Recovery and sporicidal resistance of various B. subtilis spore preparations on porcelain penicylinders compared with results from AOAC test methods.

Sporicidal test results obtained from carriers inoculated with 4 types of defined Bacillus subtilis spore preparations were compared with the standard AOAC sporicidal test using soil extract nutrient broth (SENB) B. subtilis 19659 spores. Recoveries of spores inoculated on penicylinders from B. subtilis clean spores (washed and suspended in water) and B. subtilis 19659 spores inoculated from culture filtrates according to the AOAC method were compared. Spores were exposed to 6 concentrations (0.5-3.0% w/v) of glutaraldehyde in phosphate buffer (pH 7.5) for 10 h. Concentrations were established by titrimetry and liquid chromatography. Recoveries of surviving spores were determined for 3 types of clean B. subtilis var. niger preparations, one clean B. subtilis 19659 preparation, and the SENB B. subtilis 19659 filtrates. Spore carriers, inoculated by the standard AOAC protocol, resulted in as much as a 2-log number difference in runs 1-12, but not more than 0.5 log number for each clean spore preparation. The SENB spores varied most in resistance to glutaraldehyde, with no growth in recovery media from 3 different batches of 1, 1.5, and 2% glutaraldehyde. Separate batches of SENB preparations of B. subtilis 19659 were resistant and destroyed by 1.0% glutaraldehyde, with 3.98 and 6.0 log numbers of spores on penicylinders, respectively. Clean spore preparations of B. subtilis 19659 on porcelain penicylinders were more resistant to glutaraldehyde than were SENB spores. Nutrient agar/Mg/Ca and nutrient agar/Mg spore preparations of B. subtilis var. niger showed the most uniform resistance to glutaraldehyde. Spores with calcium added showed increased resistance to glutaraldehyde. B. subtilis 19659 spores from the Columbia broth spore preparation were the most resistant and were recovered after exposure to 3.0% glutaraldehyde.

Bacillus subtilis↗
PubMedDate not supplied

Relationship of local i.v. complications and the method of intermittenti.v. access.

Infiltration, infection, phlebitis, and thrombophlebitis are considered the most frequent complications of i.v. therapy. The purpose of our study was to determine if a difference existed in incidence of complications during i.v. infusions depending on whether the i.v. tubing is directly connected to the infusion device or the tubing is connected to the latex port of an i.v. lock using a needle. Background information, mehtod followed, and results obtained in the study are discussed.

Aged↗