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Biomedical subjects

E Bailey

Publications and source records attributed to E Bailey.

At least 163 records · Page 9Linked to original sources
PubMed1982

Prevalence of anti-red blood cel antibodies in the serum and colostrum of mares and its relationship to neonatal isoerythrolysis.

The sera of 390 pregnant Standardbred mares and 409 pregnant Thoroughbred mares were tested for anti-red blood cell (RBC) antibodies. Of the Standardbred mares and Thoroughbred mares, 20% and 10%, respectively, had anti-RBC antibodies detectable in hemolytic or saline agglutination tests. Most of the antibodies were specific for the CA blood-group antigen of horses. Other antibodies were specific for the Aa, Ab, Aa, Ab, Da, Df, Ka, Ua, or Qa blood-group antigens. The occurrence of these antibodies in the serum and colostrum was compared for 268 mares. With 3 exceptions, whenever antibodies were found in 1 sample, they were found in the other. When a mare had antibodies to the Aa or Qa blood group antigens which were reactive at serum dilutions of 1:16 or greater, colostrum was withheld from that mare's foal. This practice seemed justified, because 1 foal which accidently received colostrum with anti-Aa antibodies developed neonatal isoerythrolysis. All other foals were allowed to nurse their mares' colostrum. None of them developed neonatal isoerythrolysis, even when anti-RBC antibodies were found for blood-group antigens other than Aa or Qa which reacted with the foals' RBC in in vitro tests.

Agglutination Tests↗
PubMed1981

The determination of O,S,S-trimethylphosphorodithioate in the plasma and various tissues of rats using high-resolution gas chromatography with nitrogen-phosphorus detection.

A method has been developed for the determination of O,S,S-trimethylphosphorodithioate in the plasma, lung, liver, brain and thymus of rats using high-resolution gas chromatography. The organophosphorus compound was extracted from the biological sample with ethyl acetate and analysed on a carbowax 20M fused-silica capillary column with a nitrogen-phosphorus specific detector. O,S,S-Triethylphosphorodithioate was used as an internal standard added to the sample before extraction. The sensitivity of the method allowed the compound to be measured in 0.1-ml aliquots of plasma or in 20-mg wet weight of tissue down to a level of 5 ng/sample. The method has been applied to a pharmacokinetic study in the rat after an oral or intravenous dosage with 25 mg/kg of O,S,S-trimethylphosphorodithioate.

Animals↗
PubMed1981

Determination of the antioxidant 3-tert.-butyl-4-hydroxy-anisole in rat-plasma using high-resolution gas chromatography-mass spectrometry.

A method is described for the determination of the antioxidant 3-tert.-butyl-4-hydroxy-anisole in rat plasma using high-resolution capillary gas chromatography-mass spectrometry with selective ion monitoring. Following the addition of the isomer 2-tert.-butyl-4-hydroxy-anisole, used as an internal standard, extraction was made with n-hexane and the extract derivatized with heptafluorobutyric anhydride. The gas chromatographic separation was carried out on a SE-52 fused silica capillary column and the derivatized 3-tert.-butyl-4-hydroxyanisole and its isomer detected by recording the intensities of their common fragment ion at m/e 361. The sensitivity of the method allowed the antioxidant to be measured in 0.1-ml rat plasma samples down to a level of 10 ng/ml with a high degree of specificity and accuracy. The method has been applied to a preliminary pharmacokinetic study in rats after oral dosage.

Animals↗
PubMed1981

Methylation of cysteine in hemoglobin following exposure to methylating agents.

In addition to reacting with biologically important nucleophilic sites in DNA, alkylating agents also interact with amino acids in proteins. Measurements of the extent of formation of these alkyl amino acids may be used as a means of determining exposure to these compounds. The degree of S-methylation of cysteine in hemoglobin was studied following in vivo exposure of rats to methyl methanesulfonate, dimethylnitrosamine, and 5-(3,3-dimethyl-1-triazeno)imidazole-4-carboxamide. A linear dose-response curve was observed for methyl methanesulfonate over a 100-fold dose range. For dimethylnitrosamine, there was a threshold of doses where no methylation could be detected, and a curved dose-response curve was obtained. At high doses, the degree of methylation of hemoglobin cysteine was 7-fold lower than that with methyl methanesulfonate. In vivo, no alkylation could be observed with 5-(3,3-dimethyl-1-triazeno)imidazole-4-carboxamide; however, the existence of naturally occurring S-methylcysteine in the rat hemoglobin may have overshadowed small increases in alkylation arising from exposure to this compound. The natural occurrence of S-methylcysteine was studied in 13 species, and amounts ranging from 5.6 nmol/g globin (hamster) to 481 nmol/g globin (partridge) were observed. The reason for its occurrence is unknown but is under investigation.

Alkylating Agents↗
PubMed1980

The enantiomer as internal standard for the quantitation of the alkylated amino acid S-methyl-L-cysteine in haemoglobin by gas chromatography-chemical ionisation mass spectrometry with single ion detection.

A method has been developed using gas chromatography-chemical ionisation mass spectrometry for the determination of S-methyl-L-cysteine in rat and human haemoglobin. The D-enantiomer of S-methylcysteine is added as an internal standard prior to the protein hydrolysis and a partial purification of the protein hydrolysate made by ion-exchange column chromatography. The enantiomers of S-methyl-cysteine are separated as their N-trifluoroacetyl n-butyl ester derivatives on a capillary column coated with the chiral stationary phase Chirasil-Val. The use of single ion detection in the assay is three times more sensitive than the use of multiple ion detection with a deuterium labelled internal standard. The method has been applied to haemoglobin samples from rats and humans exposed to methylating agents.

Animals↗
PubMed1980

Determination of tranylcypromine in human plasma and urine using high-resolution gas--liquid chromatography with nitrogen-sensitive detection.

A precise and sensitive method is described for the determination of the antidepressant drug tranylcypromine in human plasma and urine using high-resolution gas chromatography. The drug, together with an added internal standard, is extracted from the plasma or urine sample, derivatized with heptafluorobutyric anhydride and analysed on an OV-225 support-coated open-tubular glass capillary column with nitrogen-sensitive detection. The method has been applied to the measurement of tranylcypromine levels in plasma and urine from both healthy volunteers and from psychiatric patients receiving a therapeutic dosage of the drug.

Chromatography, Gas↗
PubMed1980

Identification and genetics of horse lymphocyte alloantigens.

Six hundred horses were tested with lymphocytotoxic antisera derived from 550 parous mares and 58 antisera produced by alloimmunization with horse blood cells. Seven equine lymphocyte specificities were identified using correlation analysis of the test data, absorption analysis and lysostripping. These specificities are expressed on lymphocytes and platelets, but not on red blood cells (RBC). Therefore, these specificities do not appear to be products of any of the eight known blood group systems of the horse. The distribution of these specificities in 113 Thoroughbred horses and 57 Arabian horses is presented. Two specificities are subtypic to two other specificities reported here. Family studies indicated that all of these specificities are products of one genetic system. However, it is not clear whether the system consists of one or more loci.

Animals↗