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E Ackerman

Publications and source records attributed to E Ackerman.

At least 55 records · Page 3Linked to original sources
PubMed1981

Transcriptional initiation and 5' termini of yeast mitochondrial RNA.

We have used vaccinia virus guanylyltransferase to label polyphosphate-terminated yeast mitochondrial RNAs in vitro with [alpha-32P]GTP. Hybridization of RNA labeled in vitro indicates the presence of multiple transcriptional initiation sites in both grande and petite mitochondrial genomes. Agarose/urea gel electrophoresis of capped RNA suggests the existence of a precursor to the small (14 S) rRNA. In contrast, direct examination of the large (21 S) rRNA by partial ribonuclease T1 digestion reveals a complete lack of processing of the 5' end of the primary transcript of this RNA.

DNA Restriction Enzymes↗
PubMed1980

The identification by affinity chromatography of the rat liver ribosomal proteins that bind to elongator and initiator transfer ribonucleic acids.

Mixed yeast elongator-tRNAs (bulk tRNA lacking fRNAm,fMet), pure isoaccepting species of elongator-tRNAs (tRNAmMet and tRNAPhe), and purified initiator-tRNA (tRNAfMet) were each oxidized with periodate and the 3' terminus was coupled to Sepharose 4B through an adipic acid dihydrazide spacer. The rat liver ribosomal proteins that associated with the tRNAs were isolated by affinity chromatography and identified by electrophoresis in polyacrylamide gels. The rat liver ribosomal proteins that were bound to the elongator-tRNA preparations were L6, L35a, and S15; small amounts of a number of other proteins also associated with the nucleic acid. When initiator-tRNA (tRNAfMet) was immobilized on Sepharose, only L6 and L35a were bound; no 40 S subunit proteins associated with initiator-tRNA. No Escherichia coli proteins formed a complex with either eukaryotic initiator- or elongator-tRNAs.

Animals↗
PubMed1979

Immunonephelometric assays.

Nephelometry, the measurement of scattered light, determines the size, shape, and concentration of the scattering particles (at least in theory). For the applications of nephelometry in immunoassays, the scatterers are the antigen-antibody complexes formed. The rationale for using nephelometry includes the advantages of greater simplicity and improved precision as compared to other assay methods. There are, however, other factors which limit the immunological assays in which nephelometry is employed, most notably its sensitivity to noise due to dust and occasional large aggregates of antigen-antibody complexes. Nephelometers commercially available for immunoassays use a variety of light sources; most measure the scattered light at a single angle, and several incorporate microcomputers. A review of the theory of light scattering by small spheres is introduced to indicate fashions in which future developments may make nephelometry the method of choice for an even wider range of immunoassays.

Humans↗
PubMed1978

Investigation of statistical decision rules for sequential hematologic laboratory tests.

A statistical data processing program for identifying patients who are likely to have abnormalities on various hematologic laboratory tests is described. The prediction of abnormal levels of serum vitamin B12, serum folate, transferrin saturation, and reticulocyte counts is based on a statistical analysis of the patient's age, sex, and routine blood cell measurements. The program was developed using data from normal-value studies and data from patients who had these laboratory abnormalities. The sensitivity and specificity of the program were evaluated in a controlled prospective study of about 5,000 ambulatory adult patients. The program's predictions also were compared with the laboratory tests requested by the patients' attending physicians. The program was most sensitive for predicting low serum vitamin B12 (74%) and low transferrin saturation (78%). In the prospective evaluation, the predictive values varied from 11% for predicting low serum folate to 65% for predicting low transferrin saturation.

Adult↗
PubMed1978

Relative merits of two electrophoretic and two column-chromatographic kets for determining serum creatine kinase isoenzyme MB activity.

Prospective analysis (by use of commercially available kits) of 339 validated admission specimens from patients consecutively admitted to a cardiac monitoring unit revealed sensitivities of 68 and 63% for two electrophoretic assays (Bioware and Helena) and up to 73 and 77% for two chromatographic assays (Roche and Worthington). Specificities were 94.8, 96,6, 90.2, and 89.7% and efficiencies were 90.2, 90.7, 87.0, and 87.0, respectively. Although these results differ from early reports, they agree well with several recent studies. However, 86 (25%) of the specimens gave at least one discrepant result based on clinical evaluation of the patients, and 68 (20%) gave at least one discrepant result between methods.

Chromatography, Ion Exchange↗
PubMed1977

Economic impact of a computer-based centralized organization in a clinical laboratory.

The Department of Laboratory Medicine, University of Washington, has explored various management of organizational approaches over the past seven years. The Centralized Processing Area supported by a computerized information system has evolved from these efforts. Despite a decrease in U.W. hospital usage since 1968-69, laboratory volume has steadily increased. The cost of these services is shown in relation both to three successive management systems and to the impact of inflation on laboratory costs. Chemistry and hematology costs per procedure, including administration and computer costs, are compared with microbiology cost per procedure, where the management system was not used. There is growing acceptance of the intangible benefits of computerization in the laboratory. Economic benefits are often presumed but have not been proven. Favorable economic effects of the Centralized Processing Area together with the computerized information system are demonstrated.

Autoanalysis↗
PubMed1977

[Not Available].

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Contraception↗
PubMed1976

An influenza simulation model for immunization studies.

A stochastic simulation epidemic model based on discrete time intervals and appropriate for any infectious agent spread by person-to-person contacts is presented. The population is highly structured, allowing for five age groups and for subgrouping mixing in families, neighborhoods, schools, and preschool playgroups as well as total community mixing. With proper choice of relative susceptibility by age, length of latency and infectivity periods, pathogenicity and withdrawal patterns, and the relative infectiousness of silent infections, the model becomes highly agent-specific. The model includes flexible immunization routines and variable vaccine response patterns. The model is applied to the 1957 Asian and 1968 Hong Kong pandemic strains of influenza A. The results of several schedules of immunization of school children are presented and compared for the two strains.

Adolescent↗