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Biomedical subjects

E A Vladimirtseva

Publications and source records attributed to E A Vladimirtseva.

At least 19 recordsLinked to original sources
PubMed1991

California serogroup viruses from mosquitoes collected in the USSR.

Four California serogroup viruses isolated from mosquitoes in the USSR were tested for antigenic analogy with prototype viruses of the California serogroup. The topotype isolates are biologically similar to, but antigenically different from each other. One is a subtype of snowshoe hare virus, two are different subtypes of Tahyna, Lumbo, and snowshoe hare viruses, and one is identical to Inkoo virus, previously isolated only in Finland. The results indicate that molecular studies of these viruses are necessary to comprehend their evolution.

Aedes↗
PubMed1988

Identity of Karelian fever and Ockelbo viruses determined by serum dilution-plaque reduction neutralization tests and oligonucleotide mapping.

The causative agents of Ockelbo disease in Sweden, Pogosta disease in Finland, and Karelian fever in the USSR have been attributed to alphaviruses (family Togaviridae) related to Sindbis virus. We compared prototypes Sindbis, Ockelbo, and Karelian fever viruses by neutralization tests. We also analyzed oligonucleotide fingerprint maps of prototypes Ockelbo and Karelian fever viruses and a strain of Sindbis virus from Czechoslovakia. The results indicate that Ockelbo and Karelian fever viruses are essentially identical and suggest that Ockelbo disease, Pogosta disease, and Karelian fever are synonyms for the same disease.

Alphavirus↗
PubMed1975

Characteristics of Sendai virus RNA transcriptive complex formed in the cytoplasm of infected ascites cells.

Virus-specific structures with sedimentation coefficients of 250-300, 200 and 150 S were isolated from the polysome fraction of Sendai virus-infected Ahrlich ascitic carcinoma cells treated with cycloheximide, at early stages of infection (1.5 to 2 hours after inoculation). All these 3 types of structure contained both parental and newly synthesized viral RNA. RNA extracted from these structures consisted of 2 components sedimenting in sucrose density gradients in the zones of 50-70 and 35-40 S. Both components contained parental and newly synthesized RNA and were partially resistant to ribonuclease. RNA extracted from rapidly sedimenting structures (250-300 S) contained mainly the 50-70 S component; RNA recovered from 200 S structures contained the 35-40 S component. By analogy with reported data, the isolated forms of RNA have been characterized as transcriptive intermediates.

Animals↗
PubMedDate not supplied

[Localization of genome RNA synthesis in Sendai virus].

Localization of Sendai virus 50S RNA in Ehrlich ascitic carcinoma cells was studied. At 48 hours postinfection virus-specific 50S RNA was found in the nucleus after 30 min exposure to 3H-uridine, and its amount increased after 1-hour exposure to the precursor reaching 18% of the total nucleus virus-specific RNA. After 2-hour or longer exposure to the precursor the amount of 50S RNA in the nuclei decreased considerably and in the cytoplasm in this gradient region a considerable radioactivity appeared. Rapid utilization of 3H-uridine by the cells under these experimental conditions suggests that 50S RNA is synthesized in the nucleus and migrates to the cytoplasm.

Animals↗
PubMedDate not supplied

[RNA-synthesizing complex of the Sendai virus in the nucleoli of infected Erlich ascitic carcinoma cells].

A virus-specific complex with sedimentation constant of 250S and buoyant density 1.35 g/cm3 in cesium chloride density gradient is formed within 20--24 hours after infection in nucleoli of Ehrlich ascitic carcinoma cells infected with Sendai virus. The complex contains a rapidly sedimenting RNA (70--90S) which is about 50% stable to RN-ase. The complex has RNA-polymerase activity in a cell-free system; the product of the RNA-polymerase reaction is a RNA with sedimentation constant 50S. It is suggested that the virus-specific structure with sedimentation constant 250 S found in nucleoli is the Sendai virus replicative complex.

Animals↗
PubMedDate not supplied

[Physicochemical properties of the RNA and proteins of an influenza virus H1N3 isolated from an ill child and antigenically analogous to A/whale/TO/19/76].

A comparative analysis of RNA and proteins of influenza A/Baku/799/82, A/whale/TO/19/76, and A/PR8/34 viruses was carried out. The viruses were shown to be similar in their polypeptide composition and oligopeptide maps of the heavy (HA1) and light (HA2) chains of hemagglutinin; in their migration properties of RNA fragments in polyacrylamide gel the A/Baku/799/82 and A/whale/TO/19/76 viruses were similar but not identical. Marked differences in the electrophoretic mobility in gel of RNA fragments coding for P proteins, HA, NP, and NA polypeptides were demonstrated. All these fragments of A/whale/TO/19/76 virus had higher electrophoretic mobility in gel. RNA fragments coding for M and NS proteins had a similar electrophoretic mobility. The A/Baku/799/82 and A/whale/TO/19/76 viruses differed considerably in migration properties of the RNA fragment coding for neuraminidase from the epidemic A/PR8/34 virus. In the latter, this fragment had a higher electrophoretic mobility in gel. Experiments of RNA-RNA hybridization demonstrated a high degree of homology of the primary structure of all RNA fragments of A/Baku/799/82 and A/whale/TO/19/76 viruses.

Animals↗
PubMedDate not supplied

[Properties of Hav6Neq2 and Hswl(H0)Hav2 influenza viruses isolated from waterfowl in southern Turkmenia].

Three influenza A virus strains were isolated from shorebirds in October, 1977, in southern Turkmenia, in the vicinities of Tedzhen water reservoir. From a common tern, A/Sterna hirundo/Turkmenia/45/77 strain was isolated with the antigenic formula Hav6Neq2, from a teal and a black-headed gull influenza A/Anas crecca/Turkmenia/4/77 and A/Larus ridibundus/Turkmenia/13/77 strains with previously unknown combination of surface antigens Hswl(H0)Nav2 were recovered. By the molecular weight of the heavy (HA1 59,000 d) and light (HA2 24,000 d) chains of hemagglutinin, the Turkmenian viruses A/Larus ridibundus/Turkmenia/13/77 and A/Anas crecca/Turkmenia/4/77 are similar to each other and to the strains having H0 hemagglutinin: A/PR8/34 (H0N1) and A/Whale/PO/19/76 (H09Nav2). The Turkmenian viruses are characterized by a low content of the light hemagglutinin chain (HA2) which is typical of the viruses with Hsw1 hemagglutinin: A/New Jersey/8/76 (Hsw1N1) and A/SW/Wisk/68 (Hsw1N1).

Animals↗