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Biomedical subjects

E A Kim

Publications and source records attributed to E A Kim.

20 records · Page 2Linked to original sources
PubMedDate not supplied

[Na,K-ATPase activity in the myocardial sarcolemma in ischemia].

The total time-controlled ischemia (up to 45 min) was studied for its effect on the Na,K-ATPase activity, content of nonesterified fatty acids (NEFA) and intensity of lipid peroxidation (LP) in sarcolemmal (SL) preparations and soluble fractions (SF) from the rat and guinea-pig left ventricles. A strong correlation between Na, K-ATPase inhibition and NEFA accumulation was revealed in the SF. On the contrary, changes in the NEFA content and LP level both in SL and SF did not correlate with a decrease in the enzymic activity. Pretreatment with albumin (0.5 mg/ml) induced equally small increase both in the control and in the ischemic SL preparations. It is suggested that the Na,K-ATPase activity during a short period of myocardial ischemia (up to 45 min) may be due to the NEFA accumulation in the cytosolic and/or extracellular space, but not in SL.

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PubMedDate not supplied

[The mechanism of formation of spectrum of nonesterified fatty acids in biological fluids of rabbits with alimentary hypercholesterolemia].

Increase in activity of fatty tissue lipoprotein lipase and blood plasma post-heparin lipoprotein lipase, decrease in activity of myocardial lipoprotein lipase as well as in the rate of intracellular lipolysis of triacylglycerols in fatty tissue, unaltered activity of liver tissue endothelial lipase were observed in rabbits with moderate alimentary hypercholesterolemia (9.63 +/- 3.48 mumole of cholesterol per ml of blood plasma as compared with 1.51 +/- 0.16 mumole/ml under conditions of normal state). The ratio of stearate was increased in composition of unesterified fatty acids from blood plasma, mesenteric lymph and myocardial sarcolemma as well as in triacylglycerols of chylomicrons total fraction, in very low density lipoproteins and their remnants. Ratios free stearate/total content of other acids correlated in blood plasma and myocardial sarcolemma (r = 0.65). Any shifts in the spectra of fatty acid residues of phospholipids were not found in myocardial membranes. In rabbits with alimentary hypercholesterolemia importance of intestine-derived triacylglycerols for production of circulating unesterified fatty acids was increased, as a result of which the ratio of various acyls, metabolized in myocardium, was altered.

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