Search PubMed⌕ Search

Biomedical subjects

E A Bogdanova

Publications and source records attributed to E A Bogdanova.

At least 19 recordsLinked to original sources
PubMed2006

[A study of human fecal microbiocenosis by experimental in vitro modeling of constipation].

A study of human fecal microbiocenosis using experimental in vitro modeling of constipation was carried out. The study revealed certain dynamics of the incidence, proportion and ratio of microorganisms isolated from the feces at various stages of cultivation. The character of the changes of these parameters depended on the type of the microorganism. According to the results of the experiment, transitory flora (staphylococci; candidas) begins to disappear within the first day of cultivation. If intestinal evacuation is retarded for more than 2 or 3 days, distortion of the microbiological characteristics of the residential microflora, bifidobacteria and escherichiae, is possible. These processes may lead to a false positive result of dysbiosis analysis. The authors conclude that dysbiotic changes in constipation are caused by fecal retention. That is why correct diagnostics of dysbiosis is possible only after regular everyday stool is reestablished.

Adult↗
PubMed2006

[The adhesive properties of lactobacteria and escherichiae in different divisions of human gastrointestinal tract under normal and pathologic conditions].

A comparison of the adhesive properties of lactobacteria and escherichiae in different divisions of human gastrointestinal tract under normal and pathologic conditions was conducted. The study shows that the population changeability of the adhesive properties of lactobacteria and escherichiae is determined by localization of the strain in the gastrointestinal tract, and morphofunctional condition of the intestinal wall. Bacteria's adhesiveness to glycocalix of intestinal epithelial cells is higher in the left half of the colon than in the right one, and is still higher in feces. Their co-adhesiveness changes in the reverse order. Bacteria localized in the parietal mucin of inflamed segments, possess low capability of forming microcolonies, and high adhesiveness to glycocalix. The results of the study demonstrate that the choice of therapeutic probiotic bacterial strains should depend on the anatomic localization of the lesion and the character of gastrointestinal pathology.

Adult↗
PubMed2006

[A study of parietal gastrointeatinal microflora of rats after oral administration of probiotics].

The article is dedicated to one of the topical problems of medical microecology, possibility of modification of parietal gastrointestinal microflora by oral administration of various probiotics, and evaluation of the effects of such modification. The results of the study show that parietal gastrointestinal microflora can be modified by oral administration of probiotics.

Administration, Oral↗
PubMed2006

[Comparison of the methods of evaluation of the microbiological danger of antimicrobial agents by the example of oligo- and polyguanidines].

The article contains the results of comparison of the methods of evaluation of the microbiological danger of antimicrobial agents by the example of oligo- and polyguanidines. The study demonstrates that choice of an adequate study model (a bacterial or protozoa species) and exposure time depends on the characteristics of the preparation to be tested. It shows that the microbocide activity of polyguanidines should be studied on a bacterial model using long (24 hours) exposure. The most sensitive to XG6 was a protozoa culture in short-time exposure. To evaluate the biological danger ofantimicrobial agents, it is also possible to apply techniques that allow revealing reversable physiological changes in a culture of test objects.

Animals↗
PubMed2006

[Study of the influence of Staphylococcus aureus on gastrointestinal tract microbiocenosis in rats].

The authors studied the modifying effect of Staphylococcus aureus on the microbial composition of gastrointestinal tract microbiocenosis. The subjects were female rats in the condition of eubiosis or dysbiosis. The species and quantitative composition of the fecal microflora and the parietal mucin in different parts of the intestine were studied after an intragastral administration of St. aureus suspension. A single introduction of St. aureus into the gastrointestinal tract of rats led to the appearance of this microbe in the feces and parietal mucin in all the parts of the intestine regardless the initial condition of the intestinal microbiocenosis. The indigenous microflora, both in eubiotic and dysbiotic conditions, practically did not respond to an intragastral administration of staphylococcus, except a little decrease in the proportion of bifidobacteria. Meanwhile, there was a significant increase in the incidence of candid detection. The indigenous parietal microflora changed more substantially, which demonstrates a higher sensitivity of the parietal microbiocenosis to a short-time exposure to an exogenous microbial factor.

Animals↗
PubMed2004

[Correction of rate and structure of ventricular rhythm in permanent atrial fibrillation: a novel pathogenetic approach].

AIM: To analyze action of digoxin and some non-digitalis drugs (beta-blockers, verapamil, amiodarone, d,l-sotalol) and their combinations on electrical activity of atria, frequency and structure of ventricular rhythm in patients with permanent atrial fibrillation. MATERIAL AND METHODS: One hundred patients with permanent atrial fibrillation and heart rate at rest above 80 bpm were divided into 9 treatment groups. High-resolution orthogonal Frank lead ECG was recorded before and after allocated treatment. Analysis included construction of ff-waves periodograms, histograms of RR interval, cardiointervalograms and application of autocorrelation function. RESULTS: It was demonstrated that frequency and form (structure) of ventricular rhythm was determined not only by the state of AV conduction but also by value of basic period of ff-waves. The mechanisms of ventricular rate deceleration by investigated drugs were not identical. beta-blockers and verapamil directly slowed AV conduction without changing parameters of ff-waves and differed from each other only in action on parameters of concealed conduction in AV node. Action of digoxin in patients with ff-waves period equal to or exceeding 0,15 s was biphasic. During phase I shortening of ff-wave period (by 0.025+/-0.012 s) occurred. This was associated with increase of their concealed conduction through AV node. The latter phenomenon represented independent mechanism of ventricular rhythm deceleration. During phase II of digoxin action direct inhibition of AV conduction took place. Amiodarone and d,l-sotalol increased basic ff-waves period. This facilitated their conduction through AV node. Greater heart rate slowing effect of d,l-sotalol was attributed to its ability to augment concealed conduction. Due to their antiarrhythmic qualities amiodarone and d,l-sotalol slowed heart rate in patients with peak RR duration in the region of 0.28-0.46 s. These patients often had bi- and tri-modal structure of interval RR histogram. Changes of ventricular rhythm structure during use of various drugs were different. Action of digoxin was most whilst that of beta-blockers least favorable. CONCLUSION: Choice of a drug for treatment of permanent atrial fibrillation should be conducted with consideration of ff-waves periodicity, parameters of RR interval histogram, and characteristics of ventricular rhythm structure.

Amiodarone↗
PubMed2004

[A study of the human parietal gastrointestinal tract microflora in health and disease].

An original technique was used to investigate the human colon mucin in different pathologies of gastrointestinal tract. The microbial pattern of the biotope in question was found to be dependable on a morphological-and-functional condition of the intestinal wall. It is noteworthy, that not only its quantitative and qualitative composition but also the nature of the inter-microbe relations and the "specific content" of microbes are subject to changes. A correlation was established between the studied characteristics of the human parietal gastrointestinal biotope and the biochemical blood parameters. The results revealed a new chain in the pathogenesis of disbiotic processes occurring in the colon mucous tunic. The diagnostic value of microbiological parameters of the human colon mucous tunic is under discussion.

Adult↗
PubMed2004

[The parietal mucin microbial population in different sections of the human gastrointestinal tract].

Microbiological examinations of the human-colon parietal mucin were made in different sections of the human gastrointestinal tract (GIT). Biopsy samples of the human colon mucus tunic were used as the research material. Convincing data were found by research on the importance of the anatomic-and-morphological factor in shaping-up of microbiocenosis of the colon-wall parietal mucin. It was established that, depending on a GIT section, not only the quantitative and qualitative composition of normal microflora but also the nature of inter-microbe interactions are subject to changes. In particular, biopsy materials of the small-intestine parietal mucin are much different from that of the colon. The colon section from the ascending colon to the sigmoid colon has, with some exceptions, an identical microbial composition. At the same time, the rectum is significantly different from other colon sections. The results are suggestive of the below cluster-type pattern of parietal biological material: microorganisms are clustered as small domains with certain specific and quantitative compositions. It was established that, although feces and parietal mucin have a similar species composition of microorganisms, still, there is a number of essential differences between them in as far as the frequency ratio and the microorganism concentration are concerned, which signifies a certain degree of isolation of the above biopsy materials. Finally, a certain isolation degree of the feces biopsy materials and of the parietal ones was established.

Bacteria↗
PubMed2004

[An analysis of the strain homogeneity of parietal biotopes of the gastrointestinal tract].

The lumen and parietal biotopes of the human gastrointestinal tract were investigated, i.e. the antibiotic sensitivity of strains E. coli and Enterococcus faecalis residing in them were comparatively analyzed. Certain isolation degrees not only of the parietal and fecal biotopes but also of certain micro-regions in the intestine wall were detected. The obtained data confirm a previously made assumption on the cluster distribution of microorganisms in the parietal mucin. The distinct strain differences between the parietal and fecal biotopes are not enough to completely extrapolate the available characteristics of the lumen microflora on the parietal microbiocenose. The above stated underlines the necessity in purpose-oriented research of parietal microbe populations of "internal" biotopes.

Adult↗
PubMed2002

[Medication reducing therapy of atrial fibrillation: a new approach to an old problem].

AIM: To study influence of different rate-reducing medicine groups on atrial electrical activity and ventricular thyrhms structure in chronic atrial fibrillation (CAF) and thus to optimize treatment. MATERIAL AND METHODS: 57 patients with CAF treated with digitalis, beta-blockers, their combinations, verapamil, amiodaron and sotalol were studied. Repeatedly highly-resolution ECG analysis was made. RESULTS: Atrial fibrillation is characterized by ff-waves basic period from 0.12 s to 0.20 s. Ventricular characteristics were determined both by AV conduction and basic ff-waves periods. The reducing of basic ff-period is the leading mechanism of digitalis rate-reducing actions in typical cases. The direct AV block appears on the late stage of therapy and is moderate. CONCLUSION: Combined therapy by medicines with different mechanisms of rate-reducing actions is the most efficient. The choice of the drug and its doses is determined by basic ff-waves periods value and the ventricular rhythm structure.

Anti-Arrhythmia Agents↗
PubMed2002

[The state of microcirculation in hypertensive disease (essential hypertension)].

Skin microcirculation was studied by laser doppler flowmetry in 149 patients with essential hypertension (92 men, 67 women; mean age 50.1+/-4.9 years) and in 25 normotensive controls. In patients with hypertension prevailed pathological hemodynamic types of microcirculation. Hyperemic, spastic and congestive types were found in 20.1, 23.5 and 40.3% of patients, respectively, while only 16.1% of patients had normal hemodynamic type. Moreover presence and severity of microcirculatory disorders depended on stage of hypertension and level of blood pressure. Thus, patients with essential hypertension had pronounced impairment of microcirculation caused by structural and functional changes of microcirculatory vascular bed.

Adult↗
PubMed1999

[Selective suppression of polymerase chain reaction].

The selective suppression of the polymerase chain reaction and methods based upon it (construction of cDNA libraries from low amounts of biological material, subtractive hybridization and differential display of mRNA, fast cloning of full-size cDNA, chromosome walking, cloning in vitro, and others) are reviewed. These methods display a high effectiveness and, taken together, enable intricate DNA analyses to be performed--from the search for nontrivial sequences to the total sequencing of the corresponding genes.

3' Untranslated Regions↗
PubMed1999

[24-hour ECG monitoring in cardiac fibrillation].

AIM: To elucidate clinical value of 24-h ECG monitoring followed by analysis of cardiac rhythm structure in patients with perpetual cardiac fibrillation (CF). MATERIALS AND METHODS: 24-h ECG monitoring has been performed 163 times in 142 patients aged 33-82 years. Interval histograms and cardiointervalograms of R-R intervals were performed. RESULTS: 24-h ECG monitoring has shown the phenomena which can be used for perfection of the diagnosis and treatment: features of 24-h changes in heart rate; features of ventricular ectopy; periods of asystole; preclinical signs of hyperdigitalization; frequency-related shift of ST segment. CONCLUSION: 24-h ECG monitoring in patients with perpetual CF provides valuable diagnostic information. Therefore, it should be conducted in all of them irrespective of subjective state and routine ECG data, especially in deciding on antiarrhythmic therapy.

Adult↗
PubMed1998

[Cloning of region-specific genetic markers of planaria using a new method--ordered differential display].

A new method for finding differentially expressed genes, termed ordered differential display of mRNAs (ODD), was used in the search for region-specific molecular markers of freshwater planarian Dugesia tigrina. In this method, the effect of selective suppression of a polymerase chain reaction (PCR) is used for the differential amplification of a pool of 3'-terminal cDNA fragments generated by digestion of cDNAs with a restriction endonuclease. In the resulting amplified cDNAs, every mRNA is represented by a cDNA fragment whose length is determined by the position of the restriction site nearest to the 3'-terminus. Subsequent PCR with primers 3'-extended by two random nucleotides allowed the amplification of 1/192 part of all cDNA molecules present in the sample. The comparison of the generated pools of cDNA molecules separated by PAGE leads to the identification of differentially expressed sequences. The systematic study of the total mRNA pool is achieved by the successive use of all possible combinations of extended primers. Some sequences preferentially expressed along the anterior-posterior axis of planarian were identified using ODD.

Animals↗
PubMed1995

Macluralisin--a serine proteinase from fruits of Maclura pomifera (Raf.) Schneid.

A serine proteinase was isolated from fruits of Maclura pomifera (Raf.) Schneid. by affinity chromatography on bacitracin-containing sorbents and gel-filtration. The enzyme, named macluralisin, is a glycoprotein with a molecular mass of 65 kDa; its protein moiety corresponds to a molecular mass of 50 kDa. The substrate specificity of macluralisin towards synthetic peptides and insulin B-chain is similar to that of cucumisin, a subtilisin-like proteinase from melon fruit. The enzyme is completely inhibited by diisopropylfluorophosphate. Its amino-acid composition resembles that of a serine proteinase isolated from the Cucurbitaceae. The N-terminal sequence has 33% of its residues identical to those of the sequence of fungal subtilisin-like proteinase K. Hence, Maclura pomifera serine proteinase belongs to the subtilisin family, which seems to be broadly distributed in the plant kingdom.

Amino Acid Sequence↗