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Biomedical subjects

E A Barnett

Publications and source records attributed to E A Barnett.

6 recordsLinked to original sources
PubMed1989

Notes on nervios: a disorder of menopause.

The condition of nerves among women in the small Peruvian town of Puente Piedre is described. Both nervios (a symptom) and Nervios (a disorder) describe a woman's difficulty with nerves. In Puente Piedre, the identification of an anxiety condition among women depends only on the women's age. Younger women with anxiety symptoms are diagnosed as nervios, a temporary condition ascribed to a single episode of high blood pressure. Menopausal women with similar symptoms, on the other hand, are thought to have a specific disorder, Nervios. In Puente Piedre, Nervios clearly is a folk illness limited to women of menopausal age.

Adult↗
PubMed1981

Germination of macroconidia and growth of Sporidesmium sclerotivorum in vitro.

Macroconidia of Sporidesmium sclerotivorum, a mycoparasite of Scleroninia spp., were induced to germinate by aqueous and ethanolic extracts of sclerotia of sclerotinia minor. Paper chromatography of sclerotial extracts indicated the presence of several amino acids and carbohydrates, chiefly glucose. Glucose was identified as the principal germination stimulant in ethanolic extracts. Glucose, fructose, mannose, cellobiose, sucrose, maltose, trehalose, soluble starch, and glycerol at 0.1% (w/v) stimulated macroconidia to germinate in 3-6 days at 25 degrees C. Crude sclerotial extracts, and glucose combined with inorganic and organic nitrogen sources, supported germination of greater numbers of macroconidia than glucose alone. Yeast extract, Casamino acids, peptone, and several carbon substrates alone did not support germination. Macroconidia germinated well (greater than 30%) over the range of pH 3-7; maximum germination (greater than 80%) occurred at pH 5.0-5.5. Mycelial growth in a glucose - Casamino acids - mineral salts medium was also greatest in the range of pH 5.0-5.5, but growth fell off sharply below pH 4.5 and above pH 6.0. The fungus grew slowly on several complex agar media adjusted to pH 5.5.

Ammonium Chloride↗
PubMed1981

Nutritional and environmental factors affecting growth and sporulation of Sporidesmium sclerotivorum.

Three of five isolates of Sporidesmium sclerotivorum, a mycoparasite of Sclerotinia spp., grew well on an agar medium containing mineral salts, glucose, thiamine, and glutamine or Casamino acids as the nitrogen source. The nitrogen requirement for two of the isolates was satisfied by NH4Cl, Casamino acids, or glutamine. Glutamine was the best single nitrogen source. Only one isolate, CS-1, was used in further nutritional studies. The optimum concentration of glutamine for growth was 5 g/L. Glucose, mannose, mannitol, and cellobiose were excellent carbon sources. A glucose concentration of 20 g/L was optimum. Mannitol supported greater growth than glucose with Casamino acids as the nitrogen source but glucose was the superior carbon source with glutamine as the nitrogen source. Greatest growth was achieved with a combination of these carbon and nitrogen sources. Sporidesmium sclerotivorum, isolate CS-1, required thiamine for growth and sporulation. Biotin stimulated growth. The fungus developed maximally within the range of pH 5.0-5.5 and growth was greatly reduced at a pH below 4.0 or above 6.0. Control of acidity by the periodic addition of NaOH solution permitted substantially increased growth. The optimum temperature for growth was 22.5-25.0 degrees C but production of macroconidia was greatest at 15-20 degrees C.

Amino Acids↗