Biomedical subjects
Publications and source records attributed to Dietrich Schimmel.
The virulence checking for P. multocida and P. haemolytica for characterization the both species is furthermore not necessary. Both species caused death in embryonated eggs. This property can be used for immunological investigations.
The immunization of laying hens caused low titer egg antibodies. These antibodies are not protective in the embryonated eggs.
Lipopolysaccharides (endotoxin) cause according to resorption out of the intestinal tract or aerogenic inhalation or by a septic infection clinical signs. The clinical reactions are praeshock symptoms, acute forms of shock and death. The experimental intratracheally administration of lipopolysaccharides into calves caused pneumonic lesions without bacterial experimental infection.
Investigations to detect the Type D dermonecrotoxin of Pasteurella (P.) multocida were first conducted on guinea pigs, following intracutaneous application of the toxin. The latter was named after the dermatonecroses observed in those early experiments. In recent years, animal experiments for toxin detection have been increasingly replaced by in vitro methods. Comparative checks on results obtained from guinea pig skin test and mouse lethality tests, on the one hand, and cell culturing, ELISA and dot-blot, on the other, revealed very close agreement between in vitro investigations and in vivo reactions. Hence, cell culture and ELISA tests can be recommended for toxin detection without any reservation, the toxin detection of (P.) multocida ssp. multocida strains for diagnostically purposes can be done without experimental animals.