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Biomedical subjects

Diana Spencer

Publications and source records attributed to Diana Spencer.

2 recordsLinked to original sources

DNA barcoding in diverse educational settings: five case studies.

Despite 250 years of modern taxonomy, there remains a large biodiversity knowledge gap. Most species remain unknown to science. DNA barcoding can help address this gap and has been used in a variety of educational contexts to incorporate original research into school curricula and informal education programmes. A growing body of evidence suggests that actively conducting research increases student engagement and retention in science. We describe case studies in five different educational settings in Canada and the USA: a programme for primary and secondary school students (ages 5-18), a year-long professional development programme for secondary school teachers, projects embedding this research into courses in a post-secondary 2-year institution and a degree-granting university, and a citizen science project. We argue that these projects are successful because the scientific content is authentic and compelling, DNA barcoding is conceptually and technically straightforward, the workflow is adaptable to a variety of situations, and online tools exist that allow participants to contribute high-quality data to the international research effort. Evidence of success includes the broad adoption of these programmes and assessment results demonstrating that participants are gaining both knowledge and confidence. There are exciting opportunities for coordination among educational projects in the future.This article is part of the themed issue 'From DNA barcodes to biomes'.

Biodiversity↗

SmartPhantom--an fMRI simulator.

Many informatics tools have emerged to process the voluminous and complex data generated by functional magnetic resonance imaging (fMRI). The interpretation of fMRI exams is largely determined by these tools. However, their performance is hard to evaluate because there is no independent means of calibration. A novel fMRI calibration system called SmartPhantom has been developed to simulate functional blood oxygen level dependent (BOLD) imaging. SmartPhantom contains a quadrature radio frequency coil, comprising two perpendicular planar loops that can be externally activated or deactivated. The system is able to produce reasonably uniform signal enhancements in a calibration sample surrounded by the two loops during an MRI scan. The enhancement is controlled well in both magnitude and predefined timing and produces BOLD-like signals. Characteristics of SmartPhantom are discussed in detail, followed by a comparison of fMRI informatics tools. Two fMRI data sets are acquired with the SmartPhantom. One with high signal-to-noise ratio provides the calibration. Another with lower SNR is input into three software packages (BrainVoyager, FSL and Statistical Parametric Mapping 2) for data preprocessing and statistical analysis. Results from the three packages are compared in both sensitivity of detecting the activation and correlation between the predicted activation and calibration.

Calibration↗