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David S Hungerford

Publications and source records attributed to David S Hungerford.

At least 19 recordsLinked to original sources

Nickel and vanadium metal ions induce apoptosis of T-lymphocyte Jurkat cells.

Metal alloys are used as prosthetic components in the orthopaedic and dental field. However, there is growing concern over the reported leaching of metal ions from implants. Ions released from metals have been thought to be associated with local immune dysfunction, inflammation, and tissue cell death. The objective of our study was to investigate whether nickel(II) and vanadium(V), present at a smaller percentage in most alloys, are cytotoxic to T-lymphocyte cell models. Jurkat T cells possess characteristics similar to human T-lymphocytes and proliferate at a faster rate. Jurkat T cells were incubated with control media alone or with concentrations of 1, 10, and 100 microg/mL of Ni(II) or V(V) for 24 h. Both types of metal ions reduced cell viability and proliferation in a dose-dependent manner. Ni(II) at 10 microg/mL and V(V) at 100 microg/mL activated Caspase-3 expression. Hoechst 33258 staining and transmission electron microscopy revealed chromatin condensation, as well as nuclear blebbing and fragmentation. Induction of DNA fragmentation by Ni(II) at 100 microg/mL was also indicated by agarose electrophoresis. Our observations indicate that Ni and V ions kill T cells via apoptotic and nonapoptotic pathways.

Apoptosis↗

What would you do? Challenges in hip surgery.

The panel reviewed cases involving both revision and primary total hip arthroplasties. The panelists are Hugh Cameron and Allan Gross from Toronto, Charles Engh from Arlington, Aaron Rosenberg from Chicago, and Bernard Stulberg from Cleveland. The cases discussed brought up some controversial issues.

Adult↗

Nontraumatic osteonecrosis of the femoral head: ten years later.

The etiology of osteonecrosis of the hip may have a genetic basis. The interaction between certain risk factors and a genetic predisposition may determine whether this disease will develop in a particular individual. The rationale for use of joint-sparing procedures in the treatment of this disease is based on radiographic measurements and findings with other imaging modalities. Early diagnosis and intervention prior to collapse of the femoral head is key to a successful outcome of joint-preserving procedures. The results of joint-preserving procedures are less satisfactory than the results of total hip arthroplasty for femoral heads that have already collapsed. New pharmacological measures as well as the use of growth and differentiation factors for the prevention and treatment of this disease may eventually alter our treatment approach, but it is necessary to await results of clinical research with long-term follow-up of these patients.

Arthroplasty↗

Systematic analysis of classification systems for osteonecrosis of the femoral head.

BACKGROUND: Multiple classification systems for osteonecrosis of the hip have been developed to assist physicians in the diagnosis and treatment of this potentially debilitating disorder. The purpose of this analysis was to delineate the classification systems utilized in reports published since 1985 and, through a comparison of the most commonly used systems, to identify consistent factors that would allow for cross-publication comparisons to be made. METHODS: We performed a PubMed search for reports of outcome studies concerning treatment methods for osteonecrosis of the hip. All studies of reported outcomes with greater than ten patients were included in the analysis. Various classification systems were tabulated to determine usage frequencies. The four most commonly used systems were then analyzed to determine common factors used for classification. RESULTS: One hundred and fifty-seven studies were available for analysis. Sixteen major classification systems that made use of more than one radiographic factor were identified, and nine of these systems had one to five modifications reported throughout the literature. Additionally, eleven other systems made use of single factors obtained from either magnetic resonance imaging or anatomic data. The review revealed that four classification systems accounted for greater than 85.4% of the reported studies. Parameters for these four systems were stratified to allow for uniformity of patient or study evaluation. CONCLUSIONS: This analysis of the reported classification systems for osteonecrosis of the femoral head revealed several similarities between the most commonly used systems. An analysis of patients can be made with any of the four major systems if specific data are collected according to various magnetic resonance imaging and radiographic findings. This approach will allow for easier comparison of studies across different centers. LEVEL OF EVIDENCE: Prognostic Level III. See Instructions to Authors on jbjs.org for a complete description of levels of evidence.

Classification↗

Survivorship of femoral revision hip arthroplasty in patients with osteonecrosis.

BACKGROUND: Recent studies have indicated that the rate of successful long-term outcome after primary total hip arthroplasty for patients with osteonecrosis may be improved with the use of later-generation porous-coated prostheses (biologic ingrowth fixation) and cement techniques (cement fixation). Less is known about the long-term outcome after revision arthroplasty in the same patient population. The purpose of this study was to characterize the clinical and radiographic outcomes of revision total hip arthroplasty in patients with osteonecrosis. METHODS: We evaluated thirty-four osteonecrotic hips in thirty patients who had undergone revision of a femoral component of a prior total hip arthroplasty. There were nineteen men (twenty-two hips) and eleven women (twelve hips) with a mean age of forty-six years. Thirty one of thirty-four hips were implanted without cement. The cementless prostheses were of different stem lengths, but thirty of thirty-one were proximally porous coated. The mean duration of follow-up was 8.2 years. Prerevision radiographs were used to determine the degree of femoral bone loss according to the classification system of Della Valle and Paprosky. The need for revision was analyzed for correlation to known risk factors for osteonecrosis, age and gender of the patient, and degree of prerevision femoral deficiency. A clinical and radiographic evaluation of outcome was performed. RESULTS: This was the first revision for twenty-seven hips, the second for five hips, and the third for two hips. Preoperatively, the defects included four Type I, nine Type II, fifteen Type IIIA, two Type IIIB, one Type IV, and three unknown. The femoral component was rerevised in twelve of the thirty-four hips. One of the failures was the only fully porous-coated stem that was implanted. One of the three cemented implants failed, as compared with eleven of the thirty-one noncemented implants. Survival rates were 90.9% at five years, 54.8% at ten years, 54.8% at fifteen years, and 27.4% at twenty years. With the small sample size, no relationship could be identified with regard to frequency of re-revision and defects, associated risk factors, patient age, or gender. CONCLUSIONS: There was a high failure rate of revised, uncemented, proximally coated femoral components in patients with osteonecrosis of the femoral head at the time of the intermediate-term follow-up. The cause of failure could not be correlated with patient age, gender, risk factors for osteonecrosis, or femoral bone stock. LEVEL OF EVIDENCE: Therapeutic Level IV. See Instructions to Authors on jbjs.org for a complete description of levels of evidence.

Adult↗

The response of osteoblast-like cells to dexamethasone and cyclic loading.

The aim of this study was to investigate whether cyclic loading can alter the response of osteoblasts to dexamethasone. Proliferation of osteoblast-like cells (MG-63) was assessed by determining uptake of 3H-thymidine. Three doses of dexamethasone were tested: 0.001 nM, .001 nM, or 0.1 nM. Exposure to increasing levels of dexamethasone resulted in decreased proliferation of osteoblast-like cells. Although there was a slight decrease in proliferation seen in the controls (no dexamethasone) with cyclic loading, cyclic loading did not affect the response of osteoblast-like cells to dexamethasone under these test conditions. Varying loading conditions and the potential effect on metabolic and synthetic activity of the osteoblast-like cells need further study.

Cell Line↗

What would you do? Case challenges in knee surgery.

These knee arthroplasty cases were presented to a panel of surgeon and they represent classic clinical problems: (1) indications for unicompartmental arthroplasty; (2) total knee arthroplasty after a high tibial osteotomy complicated by infection and extensor mechanism rupture; (3) neuropathic arthropathy; (4) posttraumatic osteoarthritis with extraarticular deformity; (5) degenerative arthritis in the young patient; (6) osteoarthritis with a valgus deformity, fixed flexion contracture, degenerative scoliosis, and leg length discrepancy.

Adult↗

Metal-on-metal resurfacing arthroplasty: no way under the sun!--in the affirmative.

I have been charged in this debate format to refute the idea suggested in the title. To do this, I will address 2 questions: (1) What problem does it solve? and (2) What risk does it pose? The answers to these questions will support my conclusion that metal-on-metal resurfacing arthroplasty is the wrong operation to be making a reappearance at this time.

Arthroplasty, Replacement, Hip↗

Ginger extract components suppress induction of chemokine expression in human synoviocytes.

INTRODUCTION: Ginger has a long history of medicinal use, particularly as an anti-inflammatory agent for a wide variety of diseases such as arthritis. Suppression of inflammation in arthritis is attributed to suppression of proinflammatory cytokines and chemokines produced by synoviocytes, chondrocytes, and leukocytes. OBJECTIVE: This study aimed to elucidate the effect of a combination ginger extract and its individual components on chemokine expression in human synoviocytes. METHODS: Human synoviocytes were incubated with 100 microg/mL combination ginger extract (GE) of Alpinia galanga (AG) and Zingiber officinale (ZO); AG extract alone; ZO extract alone; or control media, for 1 hour at 37 degrees C, 5% CO2. Cells were next activated with 1 ng/mL of tumor necrosis factor alpha (TNF-alpha) for 1 hour to determine macrophage chemotactic factor (MCP-1) and interferon-gamma activated protein (IP-10) mRNA levels using reverse transcriptase polymerase chain reaction (RT-PCR). Secreted MCP-1 and IP-10 were quantified by enzyme-linked immunosorbent assay (ELISA) following a 24 hour incubation period. RESULTS: The GE combination was consistently more effective in decreasing chemokine mRNA and chemokine secreted protein levels than its individual components ZO or AG. In comparison, ZO was more effective than AG in suppressing chemokine expression. CONCLUSION: The present study demonstrates that GE inhibits chemokine expression, and that the combination of ZO and AG components acts synergistically. This ginger formulation may be useful for suppressing inflammation due to arthritis.

Anti-Inflammatory Agents↗

Evaluation of thermoreversible polymers containing fibroblast growth factor 9 (FGF-9) for chondrocyte culture.

We previously evaluated a thermoreversible polymer gel composed of N-isopropylacrylamide and acrylic acid as a cell culture substrate and cell-delivery vehicle. The copolymer promoted phenotype expression and amplification of chondrocytes. In this study, we determined whether addition of fibroblast growth factor 9 (FGF-9), which is mitogenic for chondrocytes, would further enhance cell proliferation and phenotype expression in the polymer. We tested the hypothesis that the thermoreversible polymer containing FGF-9 would promote increased chondrocyte proliferation and phenotype expression. Articular chondrocytes (1 x 10(5)/150 microL) were plated onto control (without gel) and gel containing 24-well plates. The gels were prepared in media alone or in media containing heparin (100 microg/mL) and FGF-9 (5 microg/mL). The cultures were incubated at 37 degrees C in 5% CO(2) for 3 days. Cells remained viable in the thermoreversible polymer in the presence or absence of FGF-9. Addition of FGF-9 to the copolymer did not induce proliferation and the cell numbers did not increase. Reverse transcription polymerase chain reaction (RT-PCR)-determined expression of chondrocyte markers collagen type II and aggrecan. FGF-9 did not enhance chondrocyte proliferation nor alter the phenotype after 3 days in culture. These findings suggest the poly(NiPA-co-AAc) gel alone may provide the optimal 3D environment for propagation of chondrocytes.

Biocompatible Materials↗

Total knee arthroplasty in Ehlers-Danlos syndrome.

The Ehlers-Danlos syndromes (EDS) are a rare group of connective tissue disorders characterized by severe joint hypermobility and instability. Ten patients with 12 primary knee arthroplasties were identified. Average age at time of surgery was 43.3 years, with follow-up data acquired at an average of 65 months after surgery. Primary indications for surgery were tibiofemoral or patellar instability (n = 8) and arthritis (n = 4). Knee Society Functional scores averaged 29.6 before surgery and 51.3 at time of interview (P<.005). Knee Society Knee scores at time of follow-up evaluation averaged 70. Tibiofemoral and patella stability were significantly improved. Arthroplasty appears to be an effective option for knee arthritis and instability in EDS patients, although results and satisfaction are lower than that reported for conventional arthroplasty indications.

Adult↗

Total knee arthroplasty in obese patients: a comparison with a matched control group.

Thirty knees in 27 obese patients were matched to 30 knees in 27 nonobese patients, with both cohorts followed for a mean of approximately 15 years. Nine of 30 obese knees were revised, whereas 3 of 30 nonobese knees were revised, with Knee Society objective scores being higher in the nonobese group. There were more polyethylene insert revisions in the nonobese group, which may have been accounted for by a trend of higher activity levels in this group. The authors concluded that at long-term follow-up, although not statistically significant, there was a trend for obesity to influence the rate of aseptic loosening. This may occur because of increasing stress at the bone-prosthesis interface, whereas increased activity levels in the nonobese patients may adversely affect the longevity of the polyethylene insert.

Adult↗

Minimally invasive total hip arthroplasty: in opposition.

At the Knee Society Winter Meeting in 2003, Seth Greenwald and I debated about whether there should be new standards (ie, regulations) applied to the release of information to the public on "new developments." I argued for the public's "right to know" prior to the publication of peer-reviewed literature. He argued for regulatory constraint or "proving by peer-reviewed publication" before alerting the public. It is not a contradiction for me to currently argue against the public advertising of minimally invasive (MIS) total hip arthroplasty as not yet being in the best interest of the public. It is hard to remember a concept that has so captured both the public's and the surgical community's fancy as MIS. Patients are "demanding" MIS without knowing why. Surgeons are offering it as the next best, greatest thing without having developed the skill and experience to avoid the surgery's risks. If you put "minimally invasive hip replacement" into the Google search engine (http://www.google.com), you get 5,170 matches. If you put the same words in PubMed (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi), referencing the National Library of Medicine database, you get SEVENTEEN; none is really a peer-reviewed article. Most are 1 page papers in orthopedics from medical education meetings. On the other hand, there are over 6,000 peer-reviewed articles on total hip arthroplasty. Dr. Thomas Sculco, my couterpart in this debate, wrote an insightful editorial in the American Journal of Orthopedic Surgery in which he stated: "Although these procedures have generated incredible interest and enthusiasm, I am concerned that they may be performed to the detriment of our patients." I couldn't agree with him more. Smaller is not necessarily better and, when it is worse, it will be the "smaller" that is held accountable.

Advertising↗

Cartilage debris increases the expression of chondrodestructive tumor necrosis factor-alpha by articular chondrocytes.

PURPOSE: To determine if cartilage particles increased the expression of TNF-alpha by articular chondrocytes. TYPE OF STUDY: In vitro experiment. METHODS: Articular chondrocytes were obtained from patients undergoing primary total knee arthroplasty for osteoarthritis (n = 3) and from patients undergoing below-knee amputation for peripheral vascular disease (n = 3). Chondrocytes were then incubated with and without cartilage particles at a concentration of 5 microg/10(5) cells for 24 hours. TNF-alpha levels were then determined using reverse transcription polymerase chain reaction. RESULTS: Both normal and osteoarthritic chondrocytes had low baseline expression of TNF-alpha under standard cell culture conditions. Expression was markedly increased in response to incubation with cartilage particles, and was statistically significant. CONCLUSIONS: Cartilage debris in the traumatized and osteoarthritic joint may increase the concentration of TNF-alpha in the joint, contributing to joint symptoms and cartilage destruction. Arthroscopic debridement and lavage may improve symptoms by washing these harmful components from the joint. LEVEL OF EVIDENCE: Level IV.

Cartilage↗

Risk factors for pulmonary emboli after total hip or knee arthroplasty.

Because it is difficult to predict which patients may sustain a pulmonary embolism after total hip or knee arthroplasty, we assessed multiple thrombophilic and hypofibrinolytic parameters to identify risk factors. Twenty-nine patients who survived a known pulmonary embolism after total knee or total hip arthroplasty were matched by age, gender, race, arthritic diagnosis, procedure, and surgery date with 29 patient-controls who had a total hip or knee arthroplasty but who did not have a symptomatic known pulmonary embolism or deep vein thrombosis. Twenty-one serologic measures and five genes associated with thrombophilia, hypofibrinolysis, or both were assessed without knowledge of group assignment. All patients with pulmonary embolism had at least one abnormality of plasminogen activator inhibitor activity, dilute Russell's viper venom time, prothrombin time, or total cholesterol versus 13 of 27 (48%) control patients. Forty-seven percent of patients who experienced pulmonary embolism had at least two abnormalities of plasminogen activator inhibitor activity, dilute Russell's viper venom time, prothrombin time, or total cholesterol, versus 7% of control patients. Preoperatively, to identify patients at high risk of pulmonary embolism, plasminogen activator inhibitor activity, dilute Russell's viper venom time, prothrombin time, and cholesterol levels were most predictive. Using at least one abnormality of these four measures as a screening test to detect risk of pulmonary embolism, the test is sensitive (100%), and the predictive value of a negative test is high (100%). After additional prospective study, this may allow identification of patients at low risk (the majority of patients) in whom anticoagulation may not be required and a small group of patients at high risk for pulmonary embolism in whom prophylactic anticoagulation should be provided.

Age Distribution↗

The incorporation of morselized bone grafts in cementless acetabular revisions.

The treatment of large osteolytic lesions is a challenge during acetabular revisions. Periprosthetic bone loss can compromise the stability of new implants. The purpose of this study was to evaluate the 5- to 10-year clinical and radiographic results of morselized bone grafting for acetabular osteolysis during cementless acetabular revisions. Ninety-nine patients (108 hips) who had an acetabular revision hip arthroplasty for osteolysis were retrospectively reviewed. There were 44 men and 55 women who had a mean age of 66 years. At the index revision, the acetabular defects were debrided of granulomatous tissue and packed tightly with morselized cancellous femoral head allograft. All the revision acetabular components were implanted using cementless fixation. At a mean followup of 85 months (range, 60-118 months), the results of 103 of 108 hips (95%) were clinically and radiographically successful. The mean preoperative Harris hip score was 37 points, which improved to a mean of 91 points at the last followup. All cavitary defects had complete radiographic incorporation of the bone grafts except for two lesions. The results of this study indicate that morselized bone graft incorporates into cementless acetabular revisions. This treatment method provided a stable reconstruction in 98% of patients at a mean of 7 years followup.

Acetabulum↗