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Biomedical subjects

David Lara

Publications and source records attributed to David Lara.

3 recordsLinked to original sources

Maximum-likelihood methods in wavefront sensing: stochastic models and likelihood functions.

Maximum-likelihood (ML) estimation in wavefront sensing requires careful attention to all noise sources and all factors that influence the sensor data. We present detailed probability density functions for the output of the image detector in a wavefront sensor, conditional not only on wavefront parameters but also on various nuisance parameters. Practical ways of dealing with nuisance parameters are described, and final expressions for likelihoods and Fisher information matrices are derived. The theory is illustrated by discussing Shack-Hartmann sensors, and computational requirements are discussed. Simulation results show that ML estimation can significantly increase the dynamic range of a Shack-Hartmann sensor with four detectors and that it can reduce the residual wavefront error when compared with traditional methods.

Electrons↗

Axially resolved complete mueller matrix confocal microscopy.

We introduce a technique that is capable of obtaining complete polarization-sensitive three-dimensional images that could reveal unknown anatomical conditions of living tissue that possess polarization-dependent signatures. Previously, the 16 Mueller coefficients were measured independently only by use of two-dimensional imaging techniques. We also present the experimental combination of a depth-resolved confocal imaging system with a complete Mueller matrix polarimeter. To calibrate the system, a double-pass method had to be implemented. We also indicate, experimentally, that the confocal sectioning of the system has a degrading effect on axially resolved Mueller matrix measurements.

Algorithms↗

Double-pass axially resolved confocal Mueller matrix imaging polarimetry.

We present experimental depth-resolved complete polarization-sensitive measurements of a stack of linear retarders and glass plates by using what is to the best of our knowledge the first combination of a confocal imaging system with a complete Mueller matrix polarimeter. The axially resolved Mueller matrices were compared with a forward simulation, with good agreement.

Equipment Design↗