Potential energy and free energy landscapes.
Familiar concepts for small molecules may require reinterpretation for larger systems. For example, rearrangements between geometrical isomers are usually considered in terms of transitions between the corresponding local minima on the underlying potential energy surface, V. However, transitions between bulk phases such as solid and liquid, or between the denatured and native states of a protein, are normally addressed in terms of free energy minima. To reestablish a connection with the potential energy surface we must think in terms of representative samples of local minima of V, from which a free energy surface is projected by averaging over most of the coordinates. The present contribution outlines how this connection can be developed into a tool for quantitative calculations. In particular, stepping between the local minima of V provides powerful methods for locating the global potential energy minimum, and for calculating global thermodynamic properties. When the transition states that link local minima are also sampled we can exploit statistical rate theory to obtain insight into global dynamics and rare events. Visualizing the potential energy landscape helps to explain how the network of local minima and transition states determines properties such as heat capacity features, which signify transitions between free energy minima. The organization of the landscape also reveals how certain systems can reliably locate particular structures on the experimental time scale from among an exponentially large number of local minima. Such directed searches not only enable proteins to overcome Levinthal's paradox but may also underlie the formation of "magic numbers" in molecular beams, the self-assembly of macromolecular structures, and crystallization.