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Biomedical subjects

Danuta Gryczyńska

Publications and source records attributed to Danuta Gryczyńska.

6 recordsLinked to original sources
PubMed2006

Expression of Fas ligand and CTLA4 in adenoids has a predictive value for allergic rhinitis development in children.

BACKGROUND: The balance of CD28/CTLA4-derived signals and Fas-dependent apoptosis activity is determined by the peripheral defense mechanisms and might play a role in the pathogenesis of allergy. OBJECTIVES: The aim of the study was to investigate the expression of costimulatory and pro- and antiapoptotic molecules in adenoid T cells of children suffering from allergic rhinitis and to find out which of these molecules have a predictive value in the development of allergic rhinitis. METHODS: The adenoids of 60 children, removed because of nasal obstruction, chronic rhinitis and recurrent respiratory infection, were evaluated. Patients were divided into two groups: group 1, suffering from chronic allergic rhinitis, and group 2, suffering from chronic rhinitis, where no specific IgE was detected, including children with a positive family history of allergy (group 2a) and children with neither a personal nor a family history of allergy (group 2b). For immunohistochemical stainings anti-CD3, anti-CD19, anti-CD4, anti-CD8, anti-CD25, anti-CD28, anti-CTLA4 (CD152), anti-bcl-2, anti-Fas, and anti-FasL antibodies were used. The number of cells expressing these molecules was identified in adenoid interfollicular spaces. The results were then analyzed in allergic and nonallergic children. During a 24-month follow-up children were re-examined for allergy and results were compared to previous immunohistochemical evaluations. RESULTS: The expression of CD4, CD25, CD28, FasL, and CTLA4 was significantly increased in group 1 compared to group 2 (p < 0.05). However, the discriminant analysis confirmed that only CTLA4 and FasL expression fully discriminated allergic subjects from the others. During a 24-month period of observation 8 children from group 2a were also diagnosed with allergic rhinitis. All of them, especially those sensitized to mites, had an increased number of FasL+ and CTLA4+ in previously removed adenoids. CONCLUSION: An increased number of cells with intracellular expression of FasL and CTLA4, in interfollicular spaces of adenoids, seems to be a predictive factor of the development of allergic rhinitis.

Adenoids↗
PubMed2004

[Pediatric obstructive sleep disordered breathing].

The most common sleep disorder in children is obstructive sleep apnea syndrome (OSAS). The majority of children with OSAS improve following tonsillectomy and adenoidectomy. Diagnosis of OSAS in children and adults has difference in criteria if disease and clinical picture.

Child↗
PubMed2004

[Expression of the chosen proapoptotic and activation markers in adenoids in children with allergic rhinitis].

Naive T cells can become either tolerant or immune as a result of their first encounter with antigen. Apoptosis is the most important immunoregulatory mechanism leading to either regional tolerant or autoimmunity and allergy. The lymphatic structure closest to the nasal mucosa is the adenoid, the part of NALT - Nasal Associated Lymphoid Tissue. The objective of this study was to find evidence for our hypothesis that allergic sensitization can occur in adenoid. Of special interest the activity of proapoptotic markers of adenoids lymphocytes in interfollicular areas. In this study the number of cells presenting expression of chosen antigens in adenoids of 20 allergic and 20 non-allergic children were evaluated. The number of positive cell for CD3, CD4, CD8, CD25, CD28, CTLA4(CD152), Fas, FasL, bcl-2, bcl-x were determined using immunohistochemical staining techniques. The eosinophils in the interfollicular space showed a trend to be more numerous in the allergic group (p < 0.005). The number of CD4, CD28, FasL, CTLA-4 and bcl-x positive cells was higher in adenoids allergic than non-allergic children. The differences were statistically significant (p < 0.05; p < 0.001). The result of this study showed that the activity of pro-apoptotic and proliferation markers of the adenoid of allergic children was more intensive than in other children. These findings support that allergic sensitization either is related to or takes place in the adenoid.

Adenoids↗
PubMed2004

[Estimation the effectiveness of cefuroxime therapy in pediatric otolaryngology].

The authors present results of retrospective clinical analysis of usefulness the cefuroxime therapy of acute ENT diseases in children. The study group consist of 886 patients, aged 4 m. to 17 year, hospitalized at the Department of Paediatric Otolaryngology between 1997-2002. The efficacy of therapy was estimated on the ground of 4 degree scale. Particular attention was paid on measuring an average time of intravenous and oral administration of drug and on side effects of treatment. The results of the study shown that cefuroxime therapy is safe and effective. Beneficial therapeutic effect was obtained in 98.9% of patients.

Acute Disease↗
PubMed2003

Evaluation of CD25, CD152, Fas-ligand expression in the adenoids of allergic and non-allergic children: a pilot study.

BACKGROUND: The nasal mucosa is the initial site in the upper airways of the host defence against antigen challenge. The lymphatic structure closest to the nasal mucosa is the adenoid. Apoptotic cell death is an important mechanism for maintaining homeostasis in the immune system, and for regulating the fates of lymphocytes following encounters with self and foreign antigens. This process regulates the population of lymphocytes in peripheral lymphoid organs following exposure to various allergens. OBJECTIVES: The objectives of this study were to find evidence for the hypothesis that the development of allergic illness depends on allergic sensitisation in the adenoid. METHODS: In this study, cellular infiltrates in the adenoids of 12 allergic and 13 non-allergic children were evaluated. The number of positive cells for CD4, CD8, CD25, CD152, and Fas-ligand were determined using immunohistochemical stainings and imaging analysis techniques. RESULTS: There was found a higher expression of CD25, CD152 and Fas-ligand in small, activated lymphocytes, especially in the interfollicular area of allergic patients than in the control group. The expression of FasL in activated lymphocytes, as well as in macrophages, was observed. Statistical analysis has shown a significant correlation between expression of CD152 and allergic disease, and a more intense correlation between CD25, CD152 and Fas-ligand and allergic disease. There was no statistically important divergence in the CD4/CD8 ratio in different samples.

Adenoids↗