Training GFP to fold.
Explore the source record for details and available documents.
Directed Molecular Evolution↗
Biomedical subjects
Daniel Evanko
Publications and source records attributed to Daniel Evanko.
Using 'bumps' and 'holes' to control protein activity.
Explore the source record for details and available documents.
Animals↗
Bioluminescent quantum dots.
Bioluminescence is widely used for in vivo imaging of nude mice. By conjugating luciferase protein to quantum dots, bioluminescence resonance energy transfer (BRET) turns these useful fluorophores into a new class of bioluminescent probe.
Animals↗
Mimicking nature with protein scaffolds.
Artificial scaffolds composed of carefully chosen protein domains permit protein complexes to be assembled in vitro, providing a line of sight to construct multiprotein architectures that mimic native complexes in vivo.
Biocompatible Materials↗
Small molecules as geneticists.
Stuart Schreiber and colleagues show how small molecule sensitivities can be used to probe the natural genetic variation present in populations of organisms.
Genetic Variation↗
Optimizing your optical tweezers.
A new measurement theory for dual-trap optical tweezers shows researchers for the first time how to easily optimize their experiments to limit noise from Brownian movement.
Biopolymers↗
STEDy progress.
Continuing progress in the development of stimulated emission depletion microscopy (STED) is slowly but surely fulfilling the method's promise of ultrahigh-resolution fluorescence microscopy and may bring unexpected benefits to other imaging modalities as well.
Humans↗
In praise of manual high-throughput screening.
RNA interference (RNAi) and automated high-throughput screening is a promising combination. But the first systematic large-scale mapping of genetic interactions in an animal shows that manual methods still have advantages over sophisticated automated screens.
Animals↗
A protein alternative to RNAi.
A general method of targeting tagged proteins for rapid degradation in the cell promises to provide a powerful alternative to RNA interference (RNAi) for studying the functions of proteins in living cells.
Animals↗
Systems biology for beginners.
A web and print Focus on systems biology from Nature Publishing Group provides a practical introduction to a field that for all its promise still has many skeptics.
Cell Physiological Phenomena↗
Engineering life.
A selection strategy to produce ribosome.mRNA pairs that operate independently of the natural cellular machinery in Escherichia coli could be used for the creation of complex artificial networks in cells.
Escherichia coli↗
Primer: spying on exocytosis with amperometry.
Explore the source record for details and available documents.
Algorithms↗
Light-stimulated neurons.
Expression of an algal protein in neurons provides a means of selectively photostimulating individual neurons with unprecedented speed and reproducibility.
Algal Proteins↗