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Biomedical subjects

D Yang

Publications and source records attributed to D Yang.

At least 433 records · Page 24Linked to original sources
PubMed1992

[The patterns of the health care seeking behavior and related factors in the schizophrenic patients].

Three hundreds schizophrenic out-patients, from 6 different socioculture background psychiatric facilities in Hunan were investigated by structured interview with definite schedule. The results show that after the recognition of the problem by decision maker, 45% subjects were delayed at least one month for help-seeking, 61.3% subjects first visited the non-psychiatric agencies, and 74.3% subjects has counselled with non-psychiatric doctors or healers at least one time, especially to the healer providing superstitious or religious therapy. The help-seeking styles mainly attributes to the lower socioeconomic status of the family, inconvenient referral to the psychiatric facilities, especially if the decision maker is a female, rural resided under-educated person knowing nothing of modern psychiatry. Based on these findings, the authors urge to improve scientific psychiatric care knowledge in population, develop the resource of mental health service to facilitate the radical change of help seeking behavior of the population.

Female↗
PubMed1992

[Studies of cockroachcidal toxin].

A cockroachcidal bacterial isolate CW-W-90-3 was selected by egg yolk agar plate. The isolate produced phospholipase C (PLC) which was pathogenic to the nymph of cockroach. The conditions for production of high level PLC indicated that using LB medium supplement Tween-80 or minimal medium could effectively increase the activity of PLC. The optical phase for production of PLC was in the period of 12-18 hours and below pH8.0. The activity of PLC was reduced along with the culture time until 48 hours. The PLC was resistant to heat. The partially purified PLC from the culture supernatant was assayed by using cockroach nymphes and produced 71.74% mortality.

Animals↗
PubMed1991

Crystal structure of a bovine neurophysin II dipeptide complex at 2.8 A determined from the single-wavelength anomalous scattering signal of an incorporated iodine atom.

The crystal structure of a dipeptide complex of bovine neurophysin II has been solved at 2.8 A resolution solely by using single-wavelength anomalous scattering data from a single iodinated derivative. The asymmetric unit is an elongated tetramer of dimensions 110 x 40 x 30 A, composed of two dimers related by pseudo twofold symmetry. Each monomer consists of two homologous layers, each with four antiparallel beta-strands. The two regions are connected by a helix followed by a long loop. Monomer-monomer contacts involve antiparallel beta-sheet interactions, which form a dimer with two layers of eight beta-strands. One peptide per monomer occupies the principal hormone-binding pocket formed by part of the amino-terminal region and parts of the connecting helix and loop, with binding to protein consistent with conclusions drawn from solution studies. Dimer-dimer contacts involve the Tyr49 region adjacent to this site. A fifth dipeptide, of unknown biological significance, helps to stabilize one of the monomer-monomer interfaces and the tetramer-tetramer network in the crystal.

Amino Acid Sequence↗
PubMed1991

The structure of the gene for ribosomal protein L5 in the archaebacterium Sulfolobus acidocaldarius.

The gene for the ribosomal protein L5 from the archaebacterium Sulfolobus acidocaldarius has been isolated and sequenced. The gene codes for a basic protein of molecular weight 29 165 Da. This protein shows substantial similarity to the equivalent protein from other archaebacteria as well as from yeast, and considerably less similarity to the equivalent eubacterial protein. These results support the concept of the archaebacteria as a monophyletic kingdom more closely related to eukaryotes than to eubacteria.

Amino Acid Sequence↗
PubMed1991

Selective amplification of periportal transitional cells precedes formation of hepatocellular carcinoma in SV40 large tag transgenic mice.

In a major urinary protein (MUP)-promoter/simian virus 40 (SV40)Tag transgenic mouse line (MT-D2) the liver-directed, androgen-regulated transgene expression leads to synchronized pathology resulting in a stepwise progression to multiple hepatocellular carcinomas. SV40Tag-activated replication gives rise to two different preneoplastic alterations in hepatocytes, which are characterized in detail: 1) dysplasia and finally cell death in the original hepatocyte population and 2) amplification of periportal transitional hepatocytes leading to multifocal hyperplasia and hepatocellular carcinoma. Multifocal hyperplasia, most probably the equivalent of SV40Tag-immortalization, grows confluent and leads to hepatomegaly. SV40Tag-independent, secondary events are necessary for the tumor development from confluent hyperplasia. This allows further investigation of the steps involved in malignant transformation and progression during hepatocarcinogenesis in vivo.

Animals↗
PubMed1991

[A study of DEAE ion exchange chromatography for the purification of hen's yolk IgG].

A DEAE ion exchange chromatography procedure performed by using either gradient or stepwise elution was developed for the purification of chicken IgG antibodies from the egg yolk of immunized hens. Following this procedure, highly purified yolk IgG preparations were obtained as identified by Sephadex G 200 gel filtration as well as sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Moreover, this procedure guaranteed the recovery of antibodies of undiminished activity. The results demonstrate that the procedure is simple, reliable and practical for the yolk IgG purification. The establishment of such a method is beneficial to the production and application of egg yolk antibodies.

Animals↗
PubMed1990

The case for relationship of the flavobacteria and their relatives to the green sulfur bacteria.

Analysis of 16S rRNA sequences suggests, but does not convincingly demonstrate a specific relationship between the eubacterial phylum defined by the flavobacteria and their relatives and that defined by the green sulfur bacteria. Consequently, we have sequenced the 23S rRNA from several representatives of the former group and one of the latter in order to bring more data to bear upon this point. The 23S rRNA data alone strongly suggest a specific relationship between the two phyla, and, together with the 16S rRNA results, provides what we consider now to be a convincing case for this specific relationship.

Bacteria↗
PubMed1990

[Comparative study of two methods for the extraction of yolk IgG].

Extraction of specific IgG antibodies from egg yolks of immunized hens is a convenient and economical approach to the production of diagnostic antibody reagents. In this study, two methods for the extraction of yolk IgG were constructed and compared. That either method could extract at least 15 mg of yolk IgG preparation from 1 ml of egg yolk of hyperimmunized hens clearly indicates that both methods are reliable and efficient. Yolk IgG preparations obtained by method II were purer and possessed higher antibody activity than the yolk IgG preparations produced by method I, so method II is superior to method I. Nevertheless, the yolk IgG preparations produced by method I and method II contained 22% and 16% unrelated proteins respectively. Therefore, these two methods should primarily be used for the rough extraction of hens yolk IgG antibodies. If yolk antibody reagents with higher purity are required, the yolk IgG preparations produced by both methods should be further purified.

Animals↗
PubMed1990

[Analysis and comparison of pulp vitality after total and segmental LeFort I osteotomy].

A more systematic electrical testing of pulp vitality demonstrated that the recovery rate of pulp vitality was 89.5% after 12 months of total and segmental LeFort I osteotomy. Segmental LeFort I osteotomy only imposed an interference to the speed of pulp vitality recovery. In other words, the recovery rate of pulp vitality in segmental LeFort I osteotomy was comparable to that in total LeFort I osteotomy. Maxillary segmentation inflicted a minimum damage to its adjacent teeth if clinical manipulation was meticulous and skillful. Interdental osteotomy did not exert an apparent effect on the recovery of pulp vitality and the positive rate of pulp vitality in the teeth close to it was roughly similar with that of homonymous teeth on the opposite side or their adjacent teeth on the same side.

Adolescent↗
PubMed1990

Changes in blood lipids during six days of overfeeding with medium or long chain triglycerides.

Although medium chain triglyceride (MCT) is less calorically dense than long chain triglyceride (LCT), it produces a greater thermic effect following ingestion. We hypothesized that the previously observed high rate of thermogenesis produced by MCT overfeeding was due to hepatic de novo synthesis of long chain fatty acids (LCFA) from the excess medium chain fatty acids (MCFA). To study this, we compared the effects of overfeeding MCT- and LCT-containing diets on blood lipid profiles. Ten in-patient, nonobese males were overfed (150% of estimated energy requirements) two formula diets for 6 days each, in a randomized crossover design. Diets differed only in the composition of the fat and contained either 40% of energy as MCT or LCT (soybean oil). The major differences between diets in the resulting pattern of blood lipids were: 1) a reduction in fasting serum total cholesterol concentrations with the LCT, but not the MCT diet; and 2) a threefold increase in fasting serum triglyceride concentrations with MCT, but not LCT, diet. Moreover, 10% of the fasting triglyceride fatty acids were medium chain and 40% were 16:0 with the MCT diet. This compared to 1% and 20% for medium chain and 16:0, respectively, with the LCT diet. In addition, there were increases in 16:1, 18:0, and 18:1 in the triglycerides during MCT feeding. The changes in fatty acids in triglycerides with MCT feeding are consistent with the hypothesis that excess dietary MCT cause a significant increase in the hepatic synthesis of these fatty acids from MCFA through de novo synthesis and/or chain elongation and desaturation. These processes could account for the higher rate of postprandial thermogenesis with MCT as compared to LCT.

Adult↗
PubMed1989

In vitro percutaneous absorption and stratum corneum binding of alachlor: effect of formulation dilution with water.

The effect of vehicle dilution on the percutaneous absorption of alachlor, 2-chloro-2',6'-diethyl-N-(methoxymethyl)acetanilide, through excised human skin was determined using flowthrough design glass penetration cells and 14C radiotracer methodology. Three dilutions of alachlor (in the solubilizing commercial formulation) with distilled water were utilized: 1:20, 1:40, and 1:80 (v/v); corresponding to concentrations of 23.0, 11.8, and 5.98 mg alachlor per milliliter, respectively. Skin from the penetration studies and human powdered stratum corneum from the binding studies demonstrated a high capacity for alachlor. A soap and water (1:1, v/v) solution effectively decontaminated powdered stratum corneum. Using plasma as the receptor solution, penetration ranged from 0.5 to 4% of the applied dose for an 8-hr exposure period. Lag times of 1.2 to 1.8 hr were observed. Increasing dilution resulted in significant enhancement (p less than 0.01) in the rate and extent of alachlor penetration. Although the biological significance of this observation is unclear, this phenomenon might be explored in future studies because of its ramifications for human toxicity and for decontamination opportunities.

Acetamides↗