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D Y Ts'o

Publications and source records attributed to D Y Ts'o.

11 recordsLinked to original sources
PubMed2001

A hierarchy of the functional organization for color, form and disparity in primate visual area V2.

By combining optical imaging, single unit electrophysiology and cytochrome oxidase (CO) histology, we sought to reveal in greater detail the functional organization within the CO stripes of visual area V2 of primates. To visualize the disparity selective regions of V2, the imaging of binocular interaction was employed. These imaging maps guided single unit penetrations that then revealed a columnar organization for disparity. Our studies also showed a pattern of intermixing between the color and disparity pathways of V2, including the existence of single cells tuned for both color and disparity. While previous studies have suggested that the CO stripes of V2 constitute the fundamental organizational unit within V2, our results show a further level of organization consisting of functionally distinct subcompartments, 0.7-1.5 mm in diameter, within individual stripes. These subcompartments, which are not clearly revealed by CO histochemistry, lie within each of the thin, pale, and thick CO dense stripes in V2 and are specific for aspects of color, orientation and retinal disparity, respectively. The present results favor an architectural view of V2, not unlike that of V1, as a collection of functionally distinct subcompartments or modules situated within each of the V2 stripes. These modules also support the notion that for each cortical area (e.g. V1, V2, V4), there exists a stereotyped cortical module with a geometry that is characteristic for each area. These modules exist as a middle tier in a hierarchy of functional organization within V2.

Animals↗
PubMed1999

Specificity of color connectivity between primate V1 and V2.

To examine the functional interactions between the color and form pathways in the primate visual cortex, we have examined the functional connectivity between pairs of color oriented and nonoriented V1 and V2 neurons in Macaque monkeys. Optical imaging maps for color selectivity, orientation preference, and ocular dominance were used to identify specific functional compartments within V1 and V2 (blobs and thin stripes). These sites then were targeted with multiple electrodes, single neurons isolated, and their receptive fields characterized for orientation selectivity and color selectivity. Functional interactions between pairs of V1 and V2 neurons were inferred by cross-correlation analysis of spike firing. Three types of color interactions were studied: nonoriented V1/nonoriented V2 cell pairs, nonoriented V1/oriented V2 cell pairs, and oriented V1/nonoriented V2 cell pairs. In general, interactions between V1 and V2 neurons are highly dependent on color matching. Different cell pairs exhibited differing dependencies on spatial overlap. Interactions between nonoriented color cells in V1 and V2 are dependent on color matching but not on receptive field overlap, suggesting a role for these interactions in coding of color surfaces. In contrast, interactions between nonoriented V1 and oriented V2 color cells exhibit a strong dependency on receptive field overlap, suggesting a separate pathway for processing of color contour information. Yet another pattern of connectivity was observed between oriented V1 and nonoriented V2 cells; these cells exhibited interactions only when receptive fields were far apart and failed to interact when spatially overlapped. Such interactions may underlie the induction of color and brightness percepts from border contrasts. Our findings thus suggest the presence of separate color pathways between V1 and V2, each with differing patterns of convergence and divergence and distinct roles in color and form vision.

Animals↗
PubMed1997

Form processing modules in primate area V4.

Area V4 occupies a central position among the areas of the primate cerebral cortex involved with object recognition and analysis. Consistent with this role, neurons in V4 are selective for many visual attributes including color, orientation, and binocular disparity. However, it is uncertain whether cells within V4 are organized with respect to these properties. In this study we used in vivo optical imaging and electrophysiology in macaque visual cortex to show that cells that share certain physiological properties are indeed grouped together in V4. Our results revealed regions containing cells with common orientation selectivity. These regions were similar in size to those seen in V2 and much larger than those seen in V1 and were confirmed by appropriately targeted single-unit recording. Surprisingly, orientation organization visible through imaging was limited to the portion of V4 representing the central visual fields. Optical imaging also revealed a functional organization related to stimulus size. Size-sensitive regions (S regions) contained cells that were strongly suppressed by large stimuli. In contrast to V2, S regions in V4 contain orientation domains. These results suggest that V4 contains modular assemblies of cells related to particular aspects of form analysis. Such organization may contribute to the construction of object-based representations.

Animals↗
PubMed1995

Visual topography in primate V2: multiple representation across functional stripes.

The second visual cortical area (V2) of the primate is composed of repeating thin, pale, and thick cytochrome oxidase stripes containing primarily color-selective, broad-band oriented, and disparity-selective cells, respectively. We have now examined topography in V2 with respect to these functional subdivisions. Our data suggest that there are multiple, interleaved visual maps in V2, one for each of the color, orientation, and disparity domains. The same region of visual space is re-represented by each stripe within a stripe cycle, resulting in discontinuities or "jumps back" in representation at stripe borders. Adjacent stripe cycles represent adjacent regions of space such that the visual map is continuous from one stripe to the next like stripe. Receptive field size and scatter are significantly larger for thin stripes than for thick stripes. Unexpectedly, our data suggest two types of pale stripes within each stripe cycle, one with scatter similar to thin stripes and another to thick stripes. Some evidence also suggests the presence of multiple maps within individual stripes in V2. Consistent with functional clustering within single stripes (Ts'o et al., 1990b), we have recorded re-representations and topographic discontinuities coincident with functional borders within single stripes. These results suggest that multiple and interleaved mapping may be a common organizational strategy for representing multiple functional domains within a single cortical area.

Animals↗
PubMed1990

Functional organization of primate visual cortex revealed by high resolution optical imaging.

A high spatial resolution optical imaging system was developed to visualize cerebral cortical activity in vivo. This method is based on activity-dependent intrinsic signals and does not use voltage-sensitive dyes. Images of the living monkey striate (VI) and extrastriate (V2) visual cortex, taken during visual stimulation, were analyzed to yield maps of the distribution of cells with various functional properties. The cytochrome oxidase--rich blobs of V1 and the stripes of V2 were imaged in the living brain. In V2, no ocular dominance organization was seen, while regions of poor orientation tuning colocalized to every other cytochrome oxidase stripe. The orientation tuning of other regions of V2 appeared organized as modules that are larger and more uniform than those in V1.

Action Potentials↗
PubMed1990

Cortical functional architecture and local coupling between neuronal activity and the microcirculation revealed by in vivo high-resolution optical imaging of intrinsic signals.

We have shown previously the existence of small, activity-dependent changes in intrinsic optical properties of cortex that are useful for optical imaging of cortical functional architecture. In this study we introduce a higher resolution optical imaging system that offers spatial and temporal resolution exceeding that achieved by most alternative imaging techniques for imaging cortical functional architecture or for monitoring local changes in cerebral blood volume or oxygen saturation. In addition, we investigated the mechanisms responsible for the activity-dependent intrinsic signals evoked by sensory stimuli, and studied their origins and wavelength dependence. These studies enabled high-resolution visualization of cortical functional architecture at wavelengths ranging from 480 to 940 nm. With the use of near-infrared illumination it was possible to image cortical functional architecture through the intact dura or even through a thinned skull. In addition, the same imaging technique proved useful for imaging and discriminating sensory-evoked, activity-dependent changes in local blood volume and oxygen saturation (oxygen delivery). Illumination at 570 nm allowed imaging of activity-dependent blood volume increases, whereas at 600-630 nm, the predominant signal probably originated from activity-dependent oxygen delivery from capillaries. The onset of oxygen delivery started prior to the blood volume increase. Thus, optical imaging based on intrinsic signals is a minimally invasive procedure for monitoring short- and long-term changes in cerebral activity.

Animals↗
PubMed1988

The organization of chromatic and spatial interactions in the primate striate cortex.

The cytochrome oxidase-rich patches or blobs of the monkey striate cortex have been shown to contain cells that have unoriented receptive fields, many of which are color selective. We studied the functional organization of color opponency in the blob regions of the parafoveal representation of the visual cortex. We also examined the patterns of connectivity among blob and nonblob cells by multiple electrode penetrations and cross-correlation analysis. Some of the color-selective cells in the blobs exhibited receptive fields that were similar to those found in the parvocellular layers of the lateral geniculate nucleus (LGN): one type exhibited center-surround spatial and chromatic opponency corresponding to the Type I cell found in the LGN; another had center-only chromatic opponency, corresponding to the Type II cell of the LGN. A blob color-selective cell with no LGN counterpart had center color opponency with a nonchromatically opponent surround antagonism. We termed this cell the "modified Type II" cell. Contrary to previous reports, few true double color-opponent cells were found. Some blob cells previously characterized as double opponent probably belong to our modified Type II category and, unlike true double opponent cells, do not respond well to isoluminant color boundaries. Occasional color-selective oriented cells were either intermixed or in close proximity to blob cells. Neighboring electrode penetrations within the same blob yielded cells of the same color opponency, either red versus green or blue versus yellow, suggesting that individual blobs are dedicated to processing one color opponency. Blobs dedicated to red/green color opponency were 3 times more numerous than blue/yellow blobs. Furthermore, the cells in layer 4C lying beneath blobs of a given color opponency had identical color opponency to the overlying cells in blobs. Cross-correlation analysis of pairs of nonblob, oriented cells in the superficial layers showed interactions between cells with matched orientation and eye preference, at varying horizontal separations. Such interactions are consistent with anatomically demonstrated clustered horizontal connections. Positive cross-correlograms were found between blob cells in the same and in adjacent blobs when the cells' receptive field type, color opponency, and ocular dominance matched. Correlograms also indicated monosynaptic connections from Type II to modified Type II cells of the same color opponency, suggesting that Type II cells may contribute to the construction of the modified Type II fields in the cortex.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1986

Relationships between horizontal interactions and functional architecture in cat striate cortex as revealed by cross-correlation analysis.

Anatomical studies in the visual cortex have shown the presence of long-range horizontal connections with clustered axonal collaterals, suggesting interactions over distances of several millimeters. We used cross-correlation analysis in cat striate cortex to detect interactions between cells over comparable distances. Using one cell as a reference, we recorded from other cells with a second electrode at varying distances and looked for correlated firing between the two recording sites. This technique allowed us to combine a physiological measure of the strength and type of connection between cells with a characterization of their receptive field properties. The observed interactions were excitatory, and extended over horizontal distances of several millimeters. Furthermore, the interactions were between orientation columns of like specificity, resulting in a waxing and waning in the strength of interaction as the electrodes passed through different orientation columns. We studied relationships between strength of correlation and other receptive field properties and found a tendency for facilitatory interactions between cells sharing the same eye preference. A large proportion of our correlations was due to common input. This feature, and the similarity of interactions between cells in the same column with the reference cell, suggest a high degree of interconnectivity between and within the columns. As the distance between the two electrodes increased, the overlap of the receptive fields of the cells participating in the interactions gradually diminished. At the furthest distances recorded, the cell pairs had nonoverlapping receptive fields separated by several degrees. The distribution and range of these interactions corresponded to the clustering and extent of the horizontal connections observed anatomically.

Animals↗
PubMed1979

Computer programs for nucleic acids studies. Atomic coordinates of helices and their graphic display.

For the purpose of calculation of NMR and other physiocochemical properties of nucleic acids, a computer program in FORTRAN language has been written. This program provides the printout of the Cartesian and cylindrical coordinates of all atoms of a double-stranded helix of nucleic acid in either A, A' or B conformation with any specified base sequence up to 50 nucleotides or longer. In addition, the interatomic distances between any two atoms or distances (with both perpendicular and parallel components) from the centers of the base rings to any atom in the helix can be calculated. This information has been used for the calculation of the ring current effects of the 1H chemical shift of two short helices. Satisfactory agreement has been found in the comparison between the present data and that obtained from model construction and from the table prepared by Arter and Schmidt. The structure of the helix can also be illustrated in graphic form on a Tektronix 4006 CRT terminal. The presentation can be manipulated, such as selection, enlargement, translation and rotation.

Computers↗