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Biomedical subjects

D Wu

Publications and source records attributed to D Wu.

At least 235 records · Page 13Linked to original sources
PubMed1999

[FHIT gene is abnormal in laryngeal carcinoma].

OBJECTIVE: To determine the role of FHIT gene in laryngeal carcinogenesis. METHODS: Laryngeal cancer cell line Hep-2, HeLa cell line and 80 primary tumors from patients with laryngeal cancer were studied by means of RT-PCR, sequencing and microsatellite analysis. RESULTS: Abnormal transcripts of FHIT gene were found in the two cell lines and 70% (14/20) of primary laryngeal carcinomas. Aberrant laryngeal tumor transcripts often lack more than two exons of FHIT gene. Microsatellite analysis showed that both loss of heterozygosity and microsatellite instability of FHIT gene existed in laryngeal carcinoma and the former was more common. CONCLUSION: FHIT gene participates in the laryngeal carcinogenesis and may be one of the candidate tumor suppressor genes.

Acid Anhydride Hydrolases↗
PubMed1999

[Effects of kidney nourishing and spleen invigorating recipes on glucocorticoid receptors of pulmonary tissue and plasma corticosterone in asthmatic rats].

OBJECTIVE: To observe the effects of Guiqi San (GQS, a Chinese herbal preparation with effect of Kidney Nourishing and Spleen Invigorating), Bushen Dingchuan Tang (BSDCT, with effects of Kidney Nourishing) and Liujunzi Tang (LJZT, with effect of Spleen Invigorating) on glucocorticoid receptor (GCR) and plasma corticosterone (PCC) in asthmatic rats. METHODS: The asthmatic model of rats made by intraperitoneal injection of ovalbumin two weeks before, and the asthma consecutively challenged daily with spray inhalation of 1% ovalbumin for 7 days. The asthmatic rat treated with above-mentioned three recipes (per os) and Beclomethasone dipropionate (BDP) were taken as treated groups and the asthmatic rat as control group. GCR density of the pulmonary tissue and PCC were determined by radioligand-binding assay and competitive protein radio-binding assay. RESULTS: The RT value of GCR reduced gradually and PCC lowered also after asthma being developed. On day 7th, the three recipes could markedly up-regulate the RT of GCR and PCC. There was significant difference statistically as compared the pulmonary tissue GCR density and PCC of GQS (P < 0.01, P < 0.05), BSDCT (P < 0.05, P < 0.05) and LJZT (P < 0.05, P < 0.05) groups with those of control (asthmatic group) on the 7th day. CONCLUSION: GQS, BSDCT and LJZT played an up-regulate role of GCR density and improvement of adrenocortical function in asthmatic rats in the management of asthma attacks.

Animals↗
PubMed1999

[Dynamic monitoring of serum soluble HLA class I antigens in renal transplant recipients].

OBJECTIVE: To investigate the relations between serum soluble HLA class I antigens (sHLA-I) and the period of acute rejection and infection in renal transplant recipients. METHODS: We measured the serum levels of sHLA-I in 36 renal transplant recipients with ELISA dynamically. RESULTS: The serum sHAL-I levels were higher in uremia patients than in normal controls [(2.94 +/- 0.34) microg/L vs. (0.76 +/- 0.33) microg/L, P < 0.05]. After renal transplantation, the serum sHLA-I levels decreased significantly in stable patients [(0.63 +/- 0.33) microg/L], but increased significantly 3 days before acute rejection and 5 or 7 days after infection. CONCLUSIONS: The serum sHLA-I levels can be used as a parameter for monitoring acute rejection and infection in renal transplantation.

Adolescent↗
PubMed1999

[The study of ultrastructure of retinal pigment epithelium of transplantation].

OBJECTIVE: To investigate the ultrastructure of retinal pigment epithelium (RPE) for the base of clinical application. METHODS: Pigmented rabbit RPE was transplanted to the subretinal space of 10 unpigmented rabbits by internal route. The ultrastructure of transplanted RPE cells were observed by electron microscopy. RESULTS: The transplantation of RPE was successful. It was shown that the transplanted cells had polarity and attached to Bruch's membrane to form basal infoldings, and the relationships between transplanted cells and the host photoreceptors were also seen. CONCLUSIONS: The internal approach is a practical and useful method for transplantation of RPE. The transplanted RPE can vitalize, perform metabolism and form some normal ultrastructure.

Animals↗
PubMed1999

[The characteristics of luminance of color visual evoked potential in macular diseases].

OBJECTIVE: To objectively evaluate the luminance channel damage of macular diseases by color pattern reversal visual evoked potential (CPR-VEP). METHODS: CPR-VEP was performed in 38 eyes with age-related macular degeneration (ARMD) dry type, 24 eyes with ARMD wet type, 20 eyes with Stargardt's disease and 54 normal eyes. Color pattern stimuli were obtained with color monitor controlled by computer program. Color pattern reversal rate was 2 Hz and its spatial frequency was 0.53 cycle/degree. The color graphic system could present white/black (W/B), red/black (R/B), green/black (G/B), blue/black (B/B), yellow/black (Y/B). RESULTS: In normal eyes, CPR-VEP P(1) latency was negatively correlated with luminance, whereas P(1) amplitude positively correlated with luminance; there were linear regressions between the mean luminance and P(1) latency and between the mean luminance and P(1) amplitude. Also in ARMD dry type, the CPR-VEP showed that the characteristics of the luminance channel were similar to that in the normal eyes. However, these phenomena did not exist in ARMD wet type and Stargardt's disease. CONCLUSION: In ARMD dry type, there is no damage of luminance channel, but in ARMD wet type and Stargardt's disease, there is severe damage of luminance channel and the patients can not discriminate luminance difference.

Adolescent↗
PubMed1999

[Distribution of genotypes of alcohol dehydrogenase 2 and aldehyde dehydrogenase 2 in Japanese twin children].

OBJECTIVE: In order to prevent alcohol related deseases, this study investigated the distribution of the genes controlling alcohol metabolism in Japan's twin. METHODS: Restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) technique was used to measure the control gene of alcohol metabolized enzymes and the genotypes of alcohol dehydrogenase 2 (ADH2) and aldehyde dehydrogenase 2 (ALDH2), which were distributed in Japan's twins. At the same time, according to the difference in genotypes, the sensitive individuals were screened from the study subjects. RESULTS: The distribution of ADH2 and ALDH2 genes were consistent with the Hardy-weinberg equation. The three genotypes of ADH2 gene were ADH2(1)/ADH2(1) (1.1%), ADH2(1)/ADH2(2) (44.6%) and ADH2(2)/ADH2(2) (54.3%). And those of ALDH2 gene were ALDH2(1)/ALDH2(1) (41.3%), ALDH2(1)/ALDH2(2) (39.1%) and ALDH2(2)/ALDH2(2) (19.6%). The frequency of ADH2 and ALDH2 genes was 0.255, 0.745 and 0.609, 0.391 respectively. CONCLUSION: Not only the distribution of genotypes of ADH2 and ALDH2 is known, but also the sensitive individuals are found, which can help prevent alcohol related disease.

Alcohol Dehydrogenase↗
PubMed1999

[Hemorrheological status in patients with silicosis and its clinical significance].

OBJECTIVE: To study relationship between silicosis and hemorrheology and its clinical significance. METHODS: Varied indices for hemorrheology were measured in 85 male patients with silicosis, 45 male patients with chronic bronchitis and 100 healthy male subjects. RESULTS: Compared with the control group and the chronic bronchitis patient group, average hematocrit (Hct), whole blood apparent viscosity (eta(a)) at 4 s(-1) - 200 s(-1) shear rates, plasma viscosity (eta(p)), erythrocyte electrophoresis time (EET) and platelet electrophoresis time (PET) all significantly increased in patients with silicosis (P < 0.001). Hemorrheological indices in patients with silicosis increased with severity of disease, increased with decrease in their pulmonary function, and were all significantly higher than those in the control group (P < 0.01 approximately equal 0.001). Stepwise regression analysis showed that severity of disease correlated closely to Hct, eta(a) at 4 s(-1) - 200 s(-1), EET and PET, and that pulmonary function status in patients with silicosis and their courses correlated closely to Hct, eta(a) at 4 s(-1) - 200 s(-1), eta(p), EET and PET. CONCLUSIONS: Blood of patients with silicosis was in highly viscose status, which was easy to be complicated with multiple diseases.

Aged↗
PubMed1999

[Effects of alcohol and metabolite acetaldehyde on the proliferation of astroglial cells of fetal brain].

To explore the mechanism of the retardation and abnormality of nerve system development induced by drinking alcohol during pregnancy, 3H-TdR incorporation was used to study the proliferation of astroglial cells of fetal brain induced by alcohol and its metabolite acetaldehyde in vitro. The results showed that alcohol and acetaldehyde obviously restrain the proliferation of astrocytes. The effect of restrain induced by acetaldehyde is stronger than that by alcohol. Nerve system development retardation and abnormality resulted from alcohol is likely to be the restrain of proliferation induced by alcohol and its metabolite acetaldehyde.

Acetaldehyde↗
PubMed1999

[Chinese hamster emotionality rating scale].

To establish the rating method of Chinese Hamster emotionality and verify its reliability of assessment result so as to investigate the role of emotionality in onset/incidence of diabetes in Chinese Hamster of non-insulin-dependent diabetes mellitus, Ader's rating methods of emotionality were revised, the level of emotionality for Chinese Hamster was measured, cut off score of emotionality was computed, and reliability of rating scale was analysed. The results were that the cut off score of emotionality which was 5 scores could divide the high/low emotionality response in individual animal; Some items were moderately and highly correlated to total score (r = 0.3268-0.7347); The test-retest reliabilities of total score after 1 and 8 days ranged 0.5874 to 0.6105, the inter-rater agreement for reliability trials was 95.2%, and its Kappa value was 0.905. The results suggest that Chinese Hamster Emotionality Rating Scale has the satisfactory reliability.

Animals↗
PubMed1999

[Effect of sodium ferulate on proliferation of rabbit aortic smooth muscle cells induced by oxidized LDL].

OBJECTIVE: To investigate the effect of sodium ferulate on the proliferation and lipid peroxidation of aortic smooth muscle cells(SMC). METHOD: Using experimental models of proliferation of cultured rabbit aortic SMC induced by oxidized-low density lipoprotein, the level of lipoperoxide(LPO) was determined by thiobarbituric acid reactive substance(TBARS) method and 3H-thymidine(3H-TdR) incorporation was used to observe DNA synthesis. RESULTS: SF markedly reduced the increase of medium LPO and inhibited the 3H-TdR incorporation in a dose-dependent manner. CONCLUSION: SF can inhibit the proliferation of SMC. The inhibitory effect may be related to the inhibition of lipid peroxidation.

Angelica sinensis↗
PubMed1999

[The influence of pulsed magnetic fields on SOD's activity and MDA value in metabolism of mice].

This experiment aimed to investigate the influence of pulsed extremely-low-frequency-magnetic fields on free radical metabolism of mice. Thirty-two mice were randomly divided into four groups and were exposed to 0 T, 0.25 T, 0.34 T and 0.64 T intensity pulsed magnetic fields of 20 Hz for 40 min. The 0 T exposed group was the control group. The free radical metabolism, SOD's activity and MDA, of mice were measured respectively. The result showed the SOD's activity and MDA of the 0.34 T exposed group were both significantly lower (P < 0.01, P < 0.05) than that of the control group, while the 0.25 T and 0.64 T exposed groups were not significantly different from the control group. This demonstrates that the biological effect of pulsed extremely-low-frequency magnetic fields on free radical metabolism of mice varies and depends on the intensity of the fields. The "window" effect may exist.

Animals↗
PubMed1999

[Preparation of latices with functional group containing N and their application in rapid diagnosis of some diseases].

This article reports the preparation and diagnostic application of a variety of latices. These latices, including temary copolymer diazo latex, azidocarbonyl latex and cell labeling latex are stable, monodisperse, soap-free, and covalently combined with protein in the absence of condensing agent. We have improved diazo polystyrene latex and made ternary copolymer diazo latex for early and rapid diagnosis of some diseases. Immunoassay reagent for the dectection of dengue fever has been prepared by our lab using ternary copolymeric diazo latex and azidocarbonyl latex. The experiment showed virus antigen could be detected in 5 mins by using diazo diagnostic agent. The sensitivity of detecting patient serum sample was 82.25%; no-across reaction was observed in other inserted spread virus antigens and in serum of other feverish patient and normal control. 40 serum samples were dectected by latex agglutination assay using ternary copolymeric diazo latex immunoassay reagent. The results were compared with those of PCR and virus separation methods. The sensitivities of the three methods were 82.25%, 75% and 55% respectively; the combined sensitivity was 92.5%; higher sensitivities could be obtained by the former two methods within 1-13 days after the onset of disease.

Azides↗
PubMed1999

[Fusion expression of porcine IFN-gamma and Cysticercus cellulosae antigen cC1 in Escherichia coli cells].

AIM: To construct an expression vector, including a chimeric cDNA of porcine IFN-gamma and Cysticercus cellulosae antigen cC1. METHODS: DNA fragments of porcine IFN-gamma and cC1 including linkers were generated by polymerase chain reaction (PCR). The recombinant vector pJLA-PRcC1 was constructed by inserting a chimeric cDNA of porcine IFN-gamma and Cysticercus cellulosae antigen cC1, and transformed to E. coli XL-Blue. RESULTS: The recombinant vector was identified by restriction analysis. An inserted fragment about 1.5 kb could be found. After induction, a 52 kDa new protein band appeared in SDS-PAGE. CONCLUSION: The fusion expression of porcine IFN-gamma and cC1 antigen in Escherichia coli cells is successful.

Animals↗
PubMed1999

[Construction of DNA vaccine including a chimeric gene encoding Cysticercus cellulosae antigen and porcine interleukin-4].

AIM: To construct a fusion expression vector for DNA vaccine including porcine interleukin-4(IL-4) and antigen cC1 to enhance the protective immunity of Cysticercus cellulosae antigen cC1. METHODS: The cDNA fragments encoding porcine IL-4 and cC1 were amplified respectively by PCR and the fused. The obtained chimeric gene IL-4cC1 contained a synthetic linker of ten amino acids and the sequence surrounding its 5' AUG initiatory codon was changed to optimized translational initiation. RESULTS: Identified by restriction enzyme analysis, an insert fragment of 1.5 kb was demonstrated. It had the same sequence as reported and designed by DNA sequencing analysis. CONCLUSION: A fusion expression plasmid containing porcine IL-4 and cC1 was constructed.

Animals↗
PubMed1999

[A multichannel infrared telemetry system for physiological signal].

This paper introduces the multichannel infrared telemetry system for temperature, respiration and ECG measurements. The experimental results are given at the end of the paper. This infrared telemetry system can be used in the environment with high electromagnetic interference, such as the operating room with electrosurgical unit and become an important monitor device.

Electronics, Medical↗
PubMed1999

The study on related genes in the neoplastic transformation of immortalized human fetal tracheal fibroblast cells induced by irradiation.

In this study, we investigated the genes related to the transformation of immortalized human fetal tracheal fibroblast cell line induced by alpha particles by means of differential display mRNA method. The result revealed that there were 23 DNA fragments that were expressed intensively in alphaSHTF cells (SHTF cells forming clone on agar after irradiated by alpha particles emitted by 238Pu) only and not in SHTF (SV40-immortalized human fetal tracheal fibroblast) cells. Northern dot confirmed two fragments, C17-5, C23-1 which showed intensive mRNA expression in alphaSHTF cells, but not in SHTF cells. The length of the C17-5 fragment was 310bp. Searching in BLAST database revealed that the C17-5 fragment might be an unknown sequence.

Base Sequence↗