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Biomedical subjects

D Wise

Publications and source records attributed to D Wise.

83 records · Page 5Linked to original sources
PubMed1978

Anderson-Fabry disease: rapid detection of carriers by hair bulb analysis.

A rapid and simple method is described for the identification of the carrier state in angiokeratoma corporis diffusum. The alpha-galactosidase (alpha-D-galactoside galactohydrolase, E.C.3.2.1.22) activities in individual hair roots are measured and compared with those of N-acetyl-beta-hexosaminidase (E.C.3.2.1.30), another lysosomal enzyme that is not affected. The cellular mosaicism typical of females heterozygous for X-linked disorders is revealed by the presence of normal, affected and partially affected hair roots. Normal individuals show no affected roots, while males hemizygous for the trait have no hair roots with enzyme activities in the normal range.

Acetylglucosaminidase↗
PubMed1976

Random distribution of centromere regions at mitosis in cultured cells of Muntiacus muntjak.

The manner in which centromere regions of mitotic chromosomes are distributed with respect to the age of their DNA was studied. Cells of the Indian deer Muntiacus muntjak, were grown in the presence of bromodeoxyuridine (BrdU) for two generations and stained with the fluorescent dye Hoechst 33258. Chromatids containing "granddaughter DNA" appear dim when compared with those containing "grandparental DNA". The frequencies of the various anaphase patterns of bright and dim centromere regions were binomially distributed, indicating random distribution of chromatids with respect to the age of their DNA templates.

Animals↗
PubMed1975

Neurochemical regulation of feeding in the rat: facilitation by alpha-noradrenergic, but not dopaminergic, receptor stimulants.

Rats ate voraciously after intraventricular injections of the alpha-noradrenergic receptor stimulant clonidine. Intraventricular administration of l-norepinephrine also facilitated feeding, but similar injections of dopamine and apomorphine (a dopamine receptor stimulant) were ineffective and even tended to suppress feeding. Clonidine was 100 times more potent than norepinephrine and increased the intake of both the ordinary diet of powdered food and a highly palatable wet food. The anorexic action of amphetamine was reversed by centrally administered clonidine. These observations suggest "respondent" rather than "operant" regulation of feeding by noradrenergic systems. That is, in relation to noradrenergic mechanisms, feeding appears to be a respondent which is sensitized or disinhibited by activation of alpha-noradrenergic receptors, rather than an operant which is reinforced by the release of norepinephrine.

Adrenergic alpha-Agonists↗
PubMed1974

The lac repressor protein: molecular shape, subunit structure, and proposed model for operator interaction based on structural studies of microcrystals.

Electron microscopic and powder x-ray diffraction studies of small crystals of the lac repressor protein provide evidence on its molecular shape and subunit structure which in turn suggests a possible mode of repressor-operator interaction. The crystals are probably orthorhombic space group P222(1) with unit cell dimensions of a = 140, b = 91, c = 117 A. This tetrameric protein appears rather asymmetric, having approximate molecular dimensions of 140 A by 60 A by 45 A. The dumbbell shape of the projected molecular outline observed in the electron micrographs can be explained by assuming that the subunits are related by 222 symmetry and are placed at the corners of a plane rectangle. We propose a model for repressor-operator interaction in which the DNA binds to the repressor with its long axis aligned with that of the repressor and with its 2-fold axis coincident with a twofold axis of the repressor.

Bacterial Proteins↗
PubMedDate not supplied

The predictive validity of fundal height curves in the identification of small- and large-for-gestational-age infants.

Several investigators have developed fundal height (FH) curves which have been useful in the identification of small-for-gestational-age (SGA) infants. Two of these curves were tested to determine if they retained their predictive validity for SGA infants, and to determine their predictive validity for large-for-gestational-age (LGA) infants. Only one of the 12 SGA infants born to the 102 women in this sample would have been identified using either of the FH curves. The use of one of the FH curves would have identified all of the 10 LGA infants, while the use of the second FH curve would have identified five. Rather than assuming validity, clinicians are encouraged to test any fundal height curve for its ability to identify SGA and LGA infants in their own population.

Anthropometry↗
PubMedDate not supplied

Physicians speak out.

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Attitude of Health Personnel↗