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Biomedical subjects

D Weisshaar

Publications and source records attributed to D Weisshaar.

18 recordsLinked to original sources
PubMed2006

Wound healing complications with de novo sirolimus versus mycophenolate mofetil-based regimen in cardiac transplant recipients.

Sirolimus was introduced in de novo immunosuppression at Stanford University in view of its favorable effects on reduced rejection and cardiac allograft vasculopathy. After an apparent increase in the incidence of post-surgical wound complications as well as symptomatic pleural and pericardial effusions, we reverted to a mycophenolate mofetil (MMF)-based regimen. This retrospective study compared the outcome in heart transplant recipients on sirolimus (48 patients) with those on MMF (46 patients) in de novo immunosuppressive regimen. The incidence of any post-surgical wound complication (52% vs. 28%, p=0.019) and deep surgical wound complication (35% vs. 13%, p=0.012) was significantly higher in patients on sirolimus than on MMF. More patients on sirolimus also had symptomatic pleural (p=0.035) and large pericardial effusions (p=0.033) requiring intervention. Logistic regression analysis showed sirolimus (p=0.027) and longer cardiac bypass time (OR=1.011; p=0.048) as risk factors for any wound complication. Sirolimus in de novo immunosuppression after cardiac transplantation was associated with a significant increase in the incidence of post-surgical wound healing complications as well as symptomatic pleural and pericardial effusions.

Female↗
PubMed2005

"Safe" Coulomb excitation of 30Mg.

We report on the first radioactive beam experiment performed at the recently commissioned REX-ISOLDE facility at CERN in conjunction with the highly efficient gamma spectrometer MINIBALL. Using 30Mg ions accelerated to an energy of 2.25 MeV/u together with a thin (nat)Ni target, Coulomb excitation of the first excited 2+ states of the projectile and target nuclei well below the Coulomb barrier was observed. From the measured relative deexcitation gamma-ray yields the B(E2;0(+)gs-->2(+)1) value of 30Mg was determined to be 241(31)e2 fm4. Our result is lower than values obtained at projectile fragmentation facilities using the intermediate-energy Coulomb excitation method, and confirms the theoretical conjecture that the neutron-rich magnesium isotope 30Mg resides outside the "island of inversion."

Journal Article↗
PubMed1993

[(Partially) automated gel techniques for determining erythrocyte antigens and antibodies].

Today, in addition to the classical test tube technology to determine red blood cell antigens and antibodies, not only the microtitration plate is increasingly used, but also the gel card. Since this new technology is easy to practice and since it further provides a higher specificity and sensitivity, we investigated whether, and if so to what extent, the gel card can be automated. On the basis of our own experience spanning many years, we were able to show that antigen determinations and antibody screening can be automated. Once the samples to be tested and the tests to be performed have been recorded by electronic data processing and combined in a job list, any sampler can distribute liquids, such as reagents and serum and/or plasma as well as red blood cell suspensions, on the basis of these data. A positive sample identification is made feasible by bar coding sample tubes and gel cards; the pipetting action is organized via the bar codes. Incubation and centrifugation are of secondary importance for automation. At this time, an automatic evaluation of gel cards is not yet possible so that result interpretation must be visual and the input of results must be manual. For documentation, however, laboratory books and result interpretation reports can be printed out automatically.

Blood Grouping and Crossmatching↗
PubMed1993

[Experiences with the ES-600 equipment].

We report on the adaptation of a 'Walkaway' instrument (ES 600, Boehringer Mannheim) to be used in a blood transfusion service for donor screening of HBsAG and anti-HIV 1 + 2. Anti-CMV is obtainable, and anti-HCV will be available in the near future. Sample processing is done by a Tecan 8051 ID from barcoded primary tubes into ES 600 carousels. The capacity of this instrument is limited to 150 specimens and 600 tests per run. After mounting of reagents and control specimens, the tests are performed fully automatically without a technician. Regarding our experiences, the instrument is unanimously suited for use of blood donor testing in transfusion services with less than 145 samples to be tested daily.

Antibodies, Viral↗
PubMed1993

[Walkaway systems in general practice: Behring ELISA Processor III].

The evaluation of a new Walkaway instrument (Behring Elisa Processor III) for testing HBsAG, anti-HCV and anti-HIV 1/2 in three blood transfusion services with different sample volumes (from 60,000 to 450,000/year) gave the following results: the instrument fulfills the promises of being a real Walkaway system under routine conditions, saving labor and gaining security. The instrument must work in agreement with GLP rules, and all test steps are documented. The organization of the laboratory becomes more transparent and less flexible.

Blood Banks↗
PubMed1991

[Electronic data transfer from computer to computer in blood banks using HL7].

To date on national, european and worldwide level, different groups and committees are working on making a compatible transfer of data in all areas possible. The Working Party on Automation and Data Processing of the International Society of Blood Transfusion (ISBT) has implemented a task force, which, together with HL7 (Health Level 7), is trying to realize a practical concept for a flexible and standardized transfer of all relevant data on blood banking.

Blood Banks↗
PubMed1980

[Carboxyhaemoglobin concentrations in the recipients of banked blood (author's transl)].

The Carboxyhaemoglobin (HbCO) concentrations in banked blood may exceed 10% if the donor had previously been exposed to CO. This applies particularly to heavy smokers. The effects on the recipients of such blood were determined by means of a new method for estimating CO which involves equilibration of the alveolar gases with the partial pressure of CO in the pulmonary capillary blood during closed-circuit anaesthesia. From the values the HbCO levels in the blood of the recipient and donor can be calculated, although the latter values are only approximate. An analysis of the findings in 57 single transfusions showed that the differences in HbCO concentrations between donor and recipient varied between -0.12% and -1.5%. The length of storage of the banked blood did not affect the results. Blood from heavy smokers always induced a higher rise of the HbCO levels in the recipient's blood than did blood from non-smokers. HbCO concentrations as high as 13.5% were calculated to be present in the blood of some donors. The method, results and conclusions to be drawn from the findings are reviewed.

Anesthesia, General↗
PubMed1977

[Pre-stained slides for differential blood counts (author's transl)].

A new method for differential blood counts using pre-stained slides showed it to be suitable for routine use. To differentiate white blood cells the same experience was needed as for conventional stainings. Normal and abnormal blood pictures can be reliably distinguished. The particular advantage of the method is in the simple preparation of the smear and in avoiding the need for time-consuming staining.

Blood Cells↗
PubMed1977

Relation between concentrations of free glycerol and triglycerides in human sera.

The concentrations of total glycerol, unesterified glycerol, and glycerol deriving from triglycerides (triacylglycerols) were measured in 468 fresh human sera from unselected outpatients of a municipal hospital. The distribution mode of free glycerol was log-normal; that of triglycerides was neither normal nor log-normal. A weak but statistically approved correlation between these variables was demonstrated. A comparison of triglyceride concentrations corrected for unesterified glycerol with those based on total glycerol shows that, for the triglycerides determination, analysis for total glycerol and substraction of 0.11 mmol/liter from the resulting value appears to be justified in clinical routine. Subtraction of individual sample-blank values is unnecessary. In a triglyceride concentration range from 1.71 to 2.85 mmol/liter the 95 percentile for the possible error of this procedure ranges from + 0.04 to - 0.08 mmol/liter.

Glycerol↗
PubMed1977

[A fully enzymatic triglyceride determination in a continuous flow 12-channel analyzer (author's transl)].

A fully enzymatic triglyceride determination utilizing enzymatic hydrolysis with a lipase-esterase mixture and subsequent enzymatic glycerol determination, has been adapted for use in a continuous flow 12-channel-analyzer. The method is linear up to 7.9 mmol/l (700 mg/100 ml). The analytical precision in the concentration range of 1.5 to 5.4 mmol/l (133 to 478 mg/100 ml) is characterized by relative standard deviations of 0.8 to 4.8%. In the lower measuring range at concentrations around 0.7 mmol/l (62 mg/100 ml) a mean relative standard deviation of 7.2% is found for 1140 measurements under routine conditions. For triglyceride concentrations of 0.9 to 7.7 mmol/80 to 680 mg/100 ml) a mean relative coefficient of verspill Q = 2.0 is determined. Bilirubin caused no observable interference in the determination. In comparison with the manual method by Eggstein & Kreutz (1966) Klin. Wochenschr. 44, 262-267) the results from the fully enzymatic method on the 12-channel-analyzer were lower by approximately 16%, corrected by an additive factor of 0.029 mmol/l (2.59 mg/100 ml). The accuracy controls with controls sera showed a difference of 10%.

Autoanalysis↗