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D Weintraub

Publications and source records attributed to D Weintraub.

9 recordsLinked to original sources
PubMed1987

Evaluation of estrogen receptors by immunocytochemistry on fine-needle aspiration biopsy specimens from breast tumors.

The estrogen receptor (ER) content of 31 surgically removed breast tumors (26 duct carcinomas, one lobular carcinoma, one papillary carcinoma, one colloid carcinoma, one duct carcinoma in situ, and one atypical fibroadenoma) was determined by a commercially available immunocytochemical method (Abbott Laboratories, ER-ICA) on cytologic material obtained by fine needle aspiration biopsy (FNAB) of surgical specimens. Immunocytochemical staining of cells by a peroxidase-antiperoxidase technique was evaluated on the basis of the percentage of positive cells and the intensity of staining. An immuno-staining score for cytologic (IS-CYTO) and histologic (IS-HISTO) material was defined and a threshold of positivity determined to facilitate the semi-quantitation of results and the comparison of cases. The results of immunostaining of cytologic material were compared with the evaluation of ER in corresponding tissue samples as determined by the radioligand binding assay using the dextran-coated charcoal procedure (ER-DCC) and by ER-ICA using cryostat sections of frozen tissue. The sensitivity, specificity, predictive value of a positive test, and test efficiency of ER-ICA in cytologic material as compared to ER-DCC was 96%, 83%, 96% and 93%, respectively. The IS-CYTO was significantly correlated with the IS-HISTO in corresponding histologic material (r = 0.72, P less than 0.001). In conclusion, the combination of ER-ICA with FNAB represents a useful new technique for the evaluation of ER which may be applied to small primary tumors, tumor recurrences, and metastases.

Axilla↗
PubMed1987

[Use of immunohistochemistry in clinical cytology].

The purpose of this study was to evaluate the usefulness of immunocytochemical methods of diagnosis in cytology. Pleural effusions and ascites fluids were analyzed from 16 patients in whom the possibility of a malignant effusion was suspected. Cells obtained from the effusions were examined following routine staining by the Papanicolaou method and immunocytochemical staining to determine the presence of tissue specific antigens, i.e. common leucocyte antigen (CLA), cytokeratins (CAM 5.2, AE1), a determinant of the Human Milk Fat Globule (HMFG2) and carcinoembryonic antigen (CEA). The results of the immunocytochemistry studies were compared with the cytologic diagnosis obtained from Papanicolaou stained smears. Good agreement was observed between the diagnosis based on the interpretation of the immunocytochemical studies and the morphological diagnosis. In particular the malignant as well as the benign cells were stained with the epithelial and leucocyte markers according to their tissue origin. Anti-cytokeratin antibody stained epithelial and mesothelial cells, antibody against HFMG2 and anti-CEA stained epithelial (adenocarcinoma) but not mesothelial cells. However macrophages were also positive with anti-CEA antibody. Anti-CLA antibody stained lymphocytes, granulocytes and macrophages but did not distinguish between benign and malignant cells. Based on the results of the present study, immunocytochemical methods using a panel of tissue specific antibodies represent a useful tool in the differential diagnosis of cellular effusions.

Antigens, Neoplasm↗
PubMed1985

Mildly dilated congestive cardiomyopathy.

Five patients with only mildly dilated ventricles but other features typical of congestive cardiomyopathy underwent cardiac transplantation for class IV NYHA heart failure. The findings of clinical studies, cardiac catheterization, endomyocardial biopsy, and pathologic examination of the removed hearts in this group with mildly dilated congestive cardiomyopathy (MDCM) were compared with similar data in four patients with idiopathic restrictive cardiomyopathy (IRCM) and in 10 patients with typical dilated congestive cardiomyopathy (DCM). In comparison with the other groups, patients with MDCM had a higher incidence of familial cardiomyopathy (p = .02) and a shorter symptomatic period than patients with IRCM (p less than .02). Patients with both MDCM and DCM had globular hearts (with predominant left ventricular dilatation), congestive hemodynamics and poor left ventricular contractility (ejection fraction 12% to 19%), and high incidence of ventricular thrombi. Patients with IRCM showed normal ventricular size, marked atrial dilatation, restrictive hemodynamics, mild-to-moderate decrease in left ventricular contractility (ejection fraction 29% to 55%), and absence of ventricular thrombi. Cardiac index, ventricular wall thickness, and light microscopic findings were similar in the three groups of patients. Electron microscopy showed no myofibrillar loss in patients with IRCM but mild (partial) or moderate-to-severe (almost total) myofibrillar loss in those with MDCM and DCM, respectively. We conclude that end-stage congestive cardiomyopathy may occur without significant ventricular dilatation and patients with MDCM have heart sizes intermediate between those found in IRCM and DCM but their clinical, hemodynamic, and pathologic findings are virtually identical to those of patients with typical DCM.

Adult↗
PubMed1985

The lymphocyte subpopulations in cyclosporine-treated human heart rejection.

Monoclonal antibodies are being used as specific therapeutic agents for treating organ rejection. This type of treatment requires that the pattern and type of infiltrating cells in the graft during acute rejection be defined. In this study 24 endomyocardial biopsies from cyclosporine-treated human heart allograft recipients were obtained during acute rejection. The biopsies were stained with immunological markers to define the infiltrating cells. The biopsies were divided into three groups; mild, moderate and resolving acute rejection. In all groups T-lymphocytes (marked with the monoclonal Leu-4) were the predominant cells with absence of B-lymphocytes (marked with T015). The results of this study showed that cyclosporine-treated heart transplant recipients have a mixed suppressor/cytotoxic (Leu-2a) and helper/inducer (Leu-3) myocardial infiltrate in contrast to the biopsies of recipients treated with azathioprine, in which suppressor/cytotoxic T-lymphocytes (Leu-2a) were predominant. A clear sequential pattern of changes in the T-cell subpopulations did not emerge, although macrophages became more prevalent with resolving rejection.

Acute Disease↗
PubMed1977

Immunologic aspects of human colostrum and milk. III. Fate and absorption of cellular and soluble components in the gastrointestinal tract of the newborn.

A group of formula-fed infants were administered a single feed of poliovirus IgA antibody-rich human colostrum 18 to 72 hr after birth. Subsequently, the presence of IgG, IgA, and IgM immunoglobulin and poliovirus antibody activity was determined in serial serum and fecal samples of the neonates. Absorption of IgA immunoglobulin from the colostrum to the circulation was observed in three infants who were fed with colostrum between 18 and 24 hr after birth. Another group of infants of tuberculin-positive mothers who were being breast fed by their own mothers were followed for the development of in vitro correlates of cell-mediated immunity against tuberculin after prolonged breast feeding. Tuberculin-specific proliferative response was observed in the peripheral blood lymphocytes of two neonates after 5 weeks of breast feeding. The responses were undetectable after 12 weeks, although the infants continued to breast feed. No tuberculin reactivity was observed in the cord lymphocytes. These observations suggest uptake of IgA immunoglobulin and components of cellular immunity in the intestine during the immediate neonatal period.

Animals↗