Biomedical subjects
Publications and source records attributed to D Weber.
The research undertaken by CRAHCA results in many new techniques and ideas being adopted by medical practice managers. However, it is not merely a one-way street. In this article, six group practice managers respond to questions about how they have used and been involved with research activities the Center has developed.
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A Comprehensive Women's Center (CWC) is a concept designed to enhance the quality of both patient care and the care experience while increasing patient volumes to hospitals by detection of conditions requiring additional diagnostic interventions or therapeutic interventions. Expanding an existing Breast Center into a Comprehensive Women's Center attracts new patients by diversifying services offered from mammography and breast related services alone to providing services such as bone densitometry, cholesterol screening and even children's immunizations. A Comprehensive Women's Center also positions the hospital as the site of choice for women making healthcare decisions. The CWC will enhance the quality of women's health by increasing compliance with screening guidelines through ease of access and quality of services provided.
It has been demonstrated in mammalian systems that techniques using embryonal stem cells provide advantages over conventional injection of DNA into embryos for generation of transgenic animals. We employed cell culture approaches in an attempt to develop this technology for fish transgenesis. Using a trout embryo-derived mitogenic preparation in a specialized culture medium, we initiated replication of zebrafish blastula-derived cell cultures and expressed marker genes introduced into the cells by plasmid transfection. Reintroduction of cells from the cultures into blastula-stage embryos indicated that the cultured cells survived and may contribute to the developing organism.
Human immunodeficiency virus type 1 variants belonging to subtype A, as well as recombinant gaga/engvB variants, derived from HIV-infected patients living in the Moscow and Perm Regions, were isolated and characterized. Intravenous administration of psychoactive drugs was a major risk factor of the infection for all the patients. All the examined isolates of HIV-1 types A and A/B were shown to be characterized by a low virus-specific activity and to be used as secondary CCR5 and CXCR4 protein receptors. The findings suggest that the domination of subtype A variant in this risk group is unassociated with fundamental differences in biological properties between the isolates of this subtype and recombinant viruses.
Traces of N-nitrosopyrrolidine (NPYR) may occur in some samples of both instant coffee and fine-ground roasted coffee. The identity of NPYR in 2 samples of instant coffee was confirmed by mass spectrometry as well as by liquid chromatography-thermal energy analysis. A 2-step cleanup procedure, involving fractionation on basic alumina followed by gradient elution on reverse-phase C18 cartridge, is described that allows full-scan mass spectrometric confirmation of NPYR in tested samples.
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