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Biomedical subjects

D Watson

Publications and source records attributed to D Watson.

At least 253 records · Page 14Linked to original sources
PubMed1975

Noninvasive cardiac evaluation (NICE) of intracardiac shunts using inhaled radioactive carbon dioxide.

A totally new, noninvasive technique is presented for the detection and analysis of left-to-right cardiac shunts. Five millicuries of oxygen 15-labeled carbon dioxide is administered by a single-breath inhalation technique. The resultant lung washout curve accurately reflects the presence or absence of a shunt. The quantitative studies are promising, but the method must be compared with standard techniques in a statistically significant series. This work is in progress.

Carbon Dioxide↗
PubMed1975

Comparison of radioimmunoassay methods for human luteinising hormone.

1. Four radioimmunoassay methods for the determination of luteinising hormone in serum or urine are described. 2. Evaluation of these methods (one employing laboratory prepared reagents, three utilizing commercial kits) was made to assess their routine laboratory value. Comparable precision and accuracy were found. Great economy is achieved by pruchasing LH and anti-LH preparations and by labelling one's own hormone. 3. Suggestions for future improvements in kit LH assay control and unitage are made.

Animals↗
PubMed1974

Wound healing in potato tuber tissue: phosphon inhibition of developmental processes requiring protein synthesis.

Several aspects of wound healing in tuber tissue of potato (Solanum tuberosum var. Kennebec), known to require protein synthesis, are inhibited by 2,4-dichlorobenzyltributylphosphonium chloride (Phosphon D). Cell division was completely blocked by 60 mum Phosphon and markedly reduced by concentrations as low as 3 mum. When applied at the time of wounding, 0.25mm Phosphon completely prevented the wound-induced respiratory increase. Application at 15 hours after wounding arrested respiration at the rate present at that time. The same concentrations of Phosphon inhibited auxin-induced cell expansion of the tissue, protein synthesis as measured by the incorporation of leucine-(14)C into the trichloroacetic acid-insoluble fraction of tissue disks, and the appearance of wound-induced peroxidase isozymes. None of these inhibitory effects of Phosphon could be prevented or reversed by the application of gibberellic acid. All wound-induced processes inhibited by Phosphon are also inhibited by cycloheximide. It is suggested that inhibitory effects of Phosphon on wound healing in potato and on other developmental processes in excised plant tissues which cannot be reversed by gibberellin are due to interference with protein synthesis.

Journal Article↗
PubMed1973

A comparative study of five laboratory tests for foeto-placental dysfunction in late pregnancy.

Five foeto-placental function tests were studied in parallel in normal and abnormal late pregnancies with a view to establishing which test or tests is most satisfactorily able to identify the mother whose foetus is in danger.A critical examination of the levels in blood serum of two enzymes, placental phosphatase isoenzyme and cystine aminopeptidase, the polypeptide hormone placental lactogen (chorionic somatomammotrophin), and the oestrogen oestriol-17beta is described.A correlation was attempted beween clinical data and the results of the above laboratory analyses and also with the daily urine oestrogen output. The plasma and urine oestriol levels proved generally to be the more useful warning tests in late pregnancy, whilst the plasma cystine aminopeptidase was the least sensitive indicator of foeto-placental dysfunction.

Aminopeptidases↗