Biomedical subjects

D Wang

Publications and source records attributed to D Wang.

At least 235 records · Page 13Linked to original sources

PubMed2001

[3-D angiography in diagnosis and treatment of intracranial aneurysm].

OBJECTIVE: To summarize the results of initial application of three-dimensional angiography (3D-Angio) in diagnosis and treatment of intracranial aneurysms. METHODS: From August 1999 to January 2001, standard digital subtraction angiography (two-dimensional angiography, 2D-Angio) and 3D-Angio(obtained by reconstruction of rotational angiography) were performed in 65 patients with confirmed or suspected aneurysms. RESULTS: Sixty-six aneurysms were found in 60 patients and nothing was found in 5. Of 66 the aneurysms (60 cases), 46 (43 cases) were treated by intrasaccule embolizotion with coils, 3 (3 cases) by occluding the parent artery with detachable balloon, 10 (10 cases) by surgery, and 1 (1 case) by combination of extracranial-intracranial bypass and balloon occlusion of parent artery. Six (3 cases) were not treated. Two aneurysms that could not be detected by 2D-Angio were diagnosed by 3D-Angio. Three aneurysms that were difficult or dangerous to embolize by 2D-Angio were embolized successfully with the help of 3D-Angio. Three aneurysms seemed to be embolized on 2D-Angio were proved impossible to be embolized on 3D-Angio. Of the 46 aneurysms embolized intrasaccularly, 38(82.6%) were completely embolized on 2D-Angio. In the 38, 8 had residual aneurysm on 3D-Angio. CONCLUSION: More accurate diagnosis and secure treatment of intracranial aneurysm can be reached by 3D-Angio.

Adolescent↗

PubMed2001

[Association between renin-angiotensin system gene polymorphism and type 2 diabetics with stroke in China].

OBJECTIVE: The aim of this study was to explore the association between gene polymorphisms of renin-angiotensin system (RAS) and type 2 diabetes mellitus (DM2) complicated with stroke. METHODS: Angiotensin 1-converting enzyme (ACE) genotypes and type 1 angiotensin II receptor(AT1R) genotypes were investigated with PCR and PCR/DdeI enzyme digesting techniques in 152 cases of DM2. RESULTS: No significant differences in genotype frequencies of AT1R gene were found between DM2 complicated with stroke and that without stroke (P> 0.05). The C allele frequency of AT1R gene in DM2 with stroke was higher than that in DM2 without stroke (6.8% vs 1.9% P< 0.05; OR=3.693, 95% CI: 0.977-13.704). The ACE DD genotype and D allele frequencies were higher in DM2 with stroke than in DM2 without stroke(51.4% vs 33.3%, P<0.05; 69.6% vs 54.5%, P<0.05). There was interaction between AT1R gene A1166C polymorphism and ACE gene I/D polymorphism in the development of stroke in DM2 patients (P<0.05; OR=4.00, 95% CI: 0.733-21.838). Logistic regression analysis showed that ACE DD genotype, systolic blood pressure and serum uric acid level were risk factors of DM2 complicated with stroke. CONCLUSION: It is suggested that ACE gene I/D polymorphism is a susceptible gene for stroke in DM2 patients in China. AT1R gene A1166C polymorphism contributes to the development of stroke in DM2 patients, but it is not an independent risk factor. There is synergistic effect of AT1R A1166C and ACE I/D gene polymorphisms on the occurrence and development of stroke in type 2 diabetics in China.

Aged↗

PubMed2001

[An experimental study on the delayed resuscitation of dogs with burn shock].

OBJECTIVE: To explore the effects of delayed resuscitation on burn shock with ringer's solution. METHODS: Twelve mongrel dogs were randomly divided into control (S, n = 6) and treatment (LR, n = 6) groups. The dogs in LR group were inflicted by 35% TBSA III degree burn and were resuscitated with Lactate Ringer's solution. Urine output in amount of 1 ml.kg-1.h-1, and cardiac output at level of 70% approximately 80% of pre-burn level were referred for the modulation of the speed and amount of fluid infusion. The postburn changes of volumetric load, mean arterial pressure (MAP), LVSP +/- dp/dtmax, CI, DO2 and VO2 during the first 24 hours of resuscitation were observed. RESULTS: The infusion amount of LR solution was 8.87 +/- 1.02 ml/kg per 1% TBSA during the first 24 hours of resuscitation, which was 1.2 times more than that with Parkland formula. Furthermore, the infusion fluid amount within the first 4 hours of resuscitation was (3.63 +/- 0.99) ml.kg(-1).1% TBSA-1, which was 41% of total amount within the first 24 hours. MAP, LVSP, +/- dp/dtmax, CI, DO2 and VO2 reached or approached the levels in S group within 2 hours of resuscitation. CONCLUSION: The infusion amount of Lactate Ringer's solution needed in delayed resuscitation was larger than that in early resuscitation. And the indices of hemodynamics and myocardial mechanics were improved obviously within 2 hours of resuscitation.

Animals↗

PubMed2001

Laryngeal and hypopharyngeal carcinoma: comparison of helical CT multiplanar reformation, three-dimensional reconstruction and virtual laryngoscopy.

OBJECTIVE: To evaluate the clinical application of helical CT multiplanar reformation (MPR) three-dimensional reconstruction (3D) and virtual laryngoscopy (CTVL) in laryngeal and hypopharyngeal carcinoma. METHODS: Axial helical CT scans were performed in 22 patients with laryngeal or hypopharyngeal carcinoma, along with MPR, 3D and CTVL. These results were compared with the findings of fiber optic laryngoscopy and surgery. RESULTS: Combining axial and MPR images, both the accuracy in preoperative tumor staging and the diagnosis of metastatic lymph nodes were 95%. MPR demonstrated more information about the extent of tumor than axial images in 23% cases; 3D image displayed clearly the extension of tumor, the vessels and airway from multiple views. The location, size and extent of tumors found in cranio-caudal CTVL corresponded well with that of laryngoscopy, and CTVL demonstrated the relationship between the tumor and vocal cords and anterior commissure by caudo-cranial approach, which was inaccessible to fiber optic laryngoscopy in 3 cases. CONCLUSIONS: Axial images of helical CT clearly demonstrate the location, size and extent of laryngeal and hypopharyngeal carcinoma, while MPR and 3D images are useful in displaying the three-dimensional images and anatomical relation of the tumor. CTVL can clearly display the mucosal surface structures of the larynx and hypopharynx and is a good complementary method of laryngoscopy.

Adult↗

PubMed2001

Cytogenetic analysis in 61 couples with spontaneous abortions.

OBJECTIVE: To examine the relationship between spontaneous abortion and chromosomal abnormalities. METHODS: Couples who had one or more consecutive spontaneous abortions and had normal genitals were enrolled for cytogenetic karyotype analysis. RESULTS: In the 61 couples, the detected incidence was 11.5%, with five Robertsonian translocations, one reciprocal translocation, and one pericentric inversion of chromosome 7. CONCLUSION: Chromosomal abnormalities may play an important role in fetal wastage.

Abortion, Spontaneous↗

PubMed2001

Effects of peripheral blood mononuclear cells from idiopathic nephrotic children on cultured rat glomerular epithelial and mesangial cells.

OBJECTIVE: To investigate the effect of immune cell from idiopathic nephrotic children on extracellular matrix (ECM) synthesis by cultured rat glomerular epithelial cell (GEC) and on the proliferation of mesangial cell (GMC). METHODS: Twenty-eight children with idiopathic nephrotic syndrome and 15 age-matched healthy children were randomly selected and divided into 4 groups: Group 1, untreated nephrotic children; Group 2, glucocorticoid treated nephrotic children; Group 3, children undergoing glucocorticoid treatment with negative proteinuria; and Group 4, normal control. The peripheral blood mononuclear cells (PBMC) were collected from these children and PBMC conditioned medium (PBMC-CM) were prepared. The PBMC-CM was co-cultured with GEC and GMC respectively. The concentrations of collagen, laminin, collagen III and collagen IV in the GEC and PBMC-CM co-culture medium were investigated. The GMC proliferation was measured by the 3H-thymidin incorporation method. RESULTS: The 3H-proline incorporation coefficients of the GEC treated with the PBMC-CM of the 4 groups were 0.93, 1.24, 1.23, and 1.11, respectively. The laminin inhibitory coefficients of the 4 groups were 0.95, 1.02, 1.01, and 1.04, respectively. The inhibitory coefficients of collagen III were 0.97, 1.00, 0.99, and 1.01, respectively, for the 4 groups. All these parameters showed a significant difference between Group 1 and the other 3 groups (P < 0.05). However, there was no significant difference in the inhibitory coefficient of collagen IV between each two of the 4 groups (1.04, 1.05, 1.04, 1.08, P > 0.05). The 3H-thymidine incorporation coefficients of GMC responsive to PBMC-CM were 1.21, 1.53, 1.50, and 1.10, respectively, and no significant difference was found between the 4 groups (P > 0.05). CONCLUSION: The results suggested that the circulating immune cells from idiopathic nephrotic children have a direct effect on some ECM component synthesis in cultured rat GEC; the bio-activity of immune cells could be neutralized by administering glucocorticoid; and the circulating immune cells of nephrotic children have no direct effect on GMC proliferation.

Animals↗

PubMed2001

[CT virtual bronchoscopy in the diagnosis of central lung cancer].

OBJECTIVE: To evaluate the clinical application of CT virtual bronchoscopy(VB) in the diagnosis of centrally located lung cancer. METHODS: Forty-five cases confirmed by fiberoptic bronchoscopy and pathology underwent volume scanning using spiral CT (HiSpeed Advantage CT/i. GE Medical Systems, U.S.A). The patients examined included 33 with central lung cancer, 8 with post-operative residual bronchial stumps, 2 with post-radiotherapy lung cancer, and 2 with tracheal carcinoma. Data were transferred to computer workstation (GE Advantage Windows 3.1) and CT virtual bronchoscopic (CTVB) images with pseudocolor encoding were generated using Navigator Smooth soft-ware. RESULTS: CTVB could reveal 100% segmental bronchus and 80% subsegmental bronchus simulating fiberoptic bronchoscopy. The tumors appeared as masses or nodules, causing bronchial wall thickening and occlusions. Post-operative residual bronchial stumps showed smooth end without recurrence of tumors or appeared as nodular protrusion if tumor recurred. The post-radiotherapy bronchial wall was uneven and there was narrowing of bronchial lumen. CONCLUSION: CTVB is a noninvasive method of observing the airway. It stimulates the images observed under fiberoptic bronchoscopy and com be used partly in lieu of it. Associated with multiplanar reformat, CTVB can demonstrate extraluminal invasion of the tumor. The limitations of CTVB are that it is not possible to observe mucosal abnormalities and perform biopsy.

Adult↗

PubMed2001

[The clinical usefulness of helical CT multiplanar reformation, three-dimensional reconstruction and virtual laryngoscopy in laryngeal and hypopharyngeal carcinomas].

OBJECTIVE: To evaluate the clinical applications of helical CT multiplanar reformation (MPR), three-dimensional reconstruction (3D) and virtual laryngoscopy (CTVL) in laryngeal and hypopharyngeal carcinoma. METHODS: Axial helical CT scan was performed in 24 patients with laryngeal or hypopharyngeal carcinoma, meanwhile MPR, 3D and CTVL were performed on each patient. The results were compared with the fiberoptic laryngoscopic and surgical findings. RESULTS: Axial helical CT combined with MPR images, the accuracy of preoperative tumor staging and detection of metastatic lymph nodes were both 96%. In 25% of the cases, MPR was more informative than axial images about the extent of tumor invasion. The 3D image three-dimensionally displayed the extent of tumor invasion, and its relation to blood vessels and airway. On CTVL, the location, size and extent of intraluminal tumors of the larynx and hypopharynx when observed from above corresponded well with those observed under laryngoscope. In 4 patients, CTVL observed from below, demonstrated the relationship between the tumor and vocal cords and anterior commissure inaccessible to fiberoptic laryngoscopy. CONCLUSION: Helical CT axial scanning combined with MPR, 3D and CTVL images, can provide more comprehensive information about laryngeal and hypopharyngeal carcinoma.

Adult↗

PubMed2001

[Non-invasive prenatal diagnosis of fetal Down syndrome by dual-color fluorescence in situ hybridization with co-denaturation].

OBJECTIVE: To explore the possibility of non-invasive prenatal diagnosis of Down syndrome by dual color fluorescence in situ hybridization with co-denaturation. METHODS: Fetal nucleated red blood cells labeled by anti-glycophorin a monoclonal immunomagnetic microbeads were enriched from maternal peripheral blood by magnetic activated cell sorting. The ploidy of chromosome 21 and sex of fetuses were determined using two-color fluorescence in situ hybridization with 21 and Y chromosome probes denatured by codenaturation. The accuracy of prediction was verified according to the karyotype of fetuses by analysis of amniotic cells. RESULTS: The ploidy of chromosome 21 of fetuses from 11 pregnant women was predicted normal. The results were correctly identified by the fetal karyotypes. 5 pregnant women were predicted carrying male fetuses. The number and mean number of male fetal nucleated red blood cells were 9-65 and 25 per 2 ml, respectively. The purity of male fetal erythrocytes enriched from maternal peripheral blood was 1.4%-18.8%; 6 pregnant women were predicted carrying female fetuses, and no male fetal nucleated red blood cell was found. The results of sex prediction were consistent with the kayotypes of fetuses by analysis of amniotic cells. CONCLUSION: It is possible to predict the ploidy of chromosome 21 and the sex of fetuses when fetal nucleated erythrocytes enriched by direct immunomagnetic labeling followed by magnetic activated cell sorting are analyzed using two-color fluorescence in situ hybridization with Y and 21 chromosome probes through codenaturation.

Adult↗

PubMed2001

[Phosphorylation of gap junction gene connexin 43 protein and dissociated calcium in HeLa cell line by all-trans-retinoic acid].

OBJECTIVE: To investigate the regulation effect on signal transduction pathway of gap junction gene connexin (Cx)43 in human cervical carcinoma cell line HeLa by all-trans-retinoic acid(ATRA). METHODS: Cell culture, Fluo-3 AM loading and laser scanning confocal microscope, flowcytometer(FCM) and western blot were employed to detect expression and regulation effect on signal transduction pathway of gap junction gene connexin Cx43 in HeLa cells by retinotic acid(RA). RESULTS: After treated with ATRA, the intercellular second messenger dissociated calcium ([Ca2+]i) was much higher in HeLa cells (58.16 mumol/L) than untreated cell (35.73 mumol/L). A detectable and up-regulation of Cx43 of 43,000 protein in HeLa cells increased from 1.9% to 26.3% in RA-treated cells than untreated cell examined by FCM and western blot. Immunoblot analysis showed that only the treated cells had phosphorylated form of Cx43 protein. CONCLUSION: The anti-tumor effect of ATRA in HeLa cells might be due to up-regulation of Cx43 gene and its signal transduction pathway which medials gap junction intercellular communication.

Calcium↗

PubMed2001

[A pathologic study on the bronchioles and lung tissues in guinea pig asthma models].

OBJECTIVE: To probe further the pathologic changes in peripheral airways and lung tissues and to observe the changes of surfactant in asthma. METHODS: (1) The guinea pig asthma models were established with ovalbumin challenge method. (2) 6 groups were studied: control group, asthma group of day 1, asthma group of day 4, asthma group of day 14, intraperitoneal dexamethasone group, and budesonide inhalation group. (3) The pathologic changes were observed under optical and electron microscope. (4) The total and differential white blood cells counts of bronchoalveolar lavage fluid(BALF) were carried out. (5) The total amounts of phospholipids which represent the levels of pulmonary surfactant were measured with phosphorus detection method. RESULTS: (1) Significant hyperplasia of mucous membrane, basement membrane, and smooth muscle layer of central and peripheral airways in the asthma groups were seen. The alveolar walls were significantly thickened and alveolar spaces diminished (P < 0.001, compared with the control group). Significant infiltration of eosinophils and lymphocytes were observed in airways and lung tissues. (2) The total cell number and the number eosinophils and lymphocytes in BALF were significantly increased in the asthma groups than those in the control group(P < 0.01). Neutrophils were dominant at early stage, but eosinophils and lymphocytes at late stage. (3) The ultrastructural changes of type II alveolar cells in asthma group included cell swelling, evacuation of lamellar bodies and partial cell desquamation. (4) The total amount of phospholipids in BALF was significantly decreased in the asthma groups as compared with that in the control group (P < 0.01). The above changes were significantly improved in both corticosteroid treated groups. CONCLUSION: Widespread and significant inflammation with eosinophilic infiltration exists in the bronchioles and alveolar walls in guinea pig asthma models. Not only there is structural change, but also the function of alveolar cells is influenced. Asthma is a disease involving the whole airway and lung system.

Animals↗

PubMed2001

[Pulmonary thromboendarterectomy in the management of chronic pulmonary thromboembolic hypertension].

OBJECTIVE: The efficacy of surgical treatment (pulmonary thromboendarterectomy, PTE) in the management of chronic pulmonary thromboembolic hypertension (CTE-PH) was reviewed. METHODS: A retrospective study of 8 patients receiving consecutive PTE in Fuwai cardiovascular hospital was made, with special regard to the operation methods, perioperative management and follow-up. RESULTS: All survived the operation. As compared with preoperative values, all patients presented with significant improvement of systolic pulmonary artery pressure [(39 +/- 15) mmHg versus (101 +/- 24) mm Hg], arterial oxygen tension [(92 +/- 7) mmHg versus (59 +/- 11) mm Hg] and arterial oxygen saturation (0.98 +/- 0.01 versus 0.89 +/- 0.06). Mid-term follow-up showed that the cardiac function of the patients fell into NYHA class I (n = 3) or II (n = 5), with significant improvement in quality of life. CONCLUSION: PTE is effective treatment in the management of CTE-PH.

Adult↗

PubMed2001

[Changes of crop yields and soil fertility under long-term application of fertilizer and recycled nutrients in manure on a black soil].

The results from a field experiment over thirteen years on a black soil in Northeast China indicated that nitrogen fertilizer increased crop yields by 724 kg.hm-2 per year on average, with an increase rate of 27.5% and about 9.4 kg increased yield from 1 kg applied N. In the early stage, the crop yields almost gave no response to phosphorus fertilizer, owing to the high level of soil available phosphorus at the beginning of the experiment. The average increase of crop yields by phosphorus in thirteen years was only 241 kg.hm-2 per year, with an increase rate of 7%, about 12.7 kg increased yield from 1 kg applied P. Annual application of recycled pig manure made by 80% of harvested grain fed to pigs and the stolks as bedding materials could increase crop yields by 268, 258 and 255 kg.hm-2 per year with an increase rate of 9.8%, 7.6% and 7.0%, based on no fertilizer, with N and with P and N, respectively. There was a trend that the effect of recycled manure on crop yield was gradually increased during the period of experiment, implying the existence of accumulative residual effect from the manure.

Agriculture↗

PubMed2001

[Damage and economic threshold of the third generation of cotton bollworm in the Yangtze River basin cotton region of Jiangsu Province].

The impact of the third generation of cotton bollworm Helicoverpa armigera on shedding of buds and bolls and on bolling was studied by inoculating its larvae to cotton plants. The results showed that the shed rate of buds and bolls damaged by the larvae had the greatest impact on bolling, and its direct path coefficient was up to -0.8917. The larval population density and the number of buds and bolls damaged by the larvae impacted bolling mainly through the damaged shed rate during inoculating stage, and their indirect path coefficients were -0.8894 and -0.891 respectively. Both of the direct and indirect path coefficients of natural shed rate during inoculating stage to bolling were small, which showed that the cotton had little capacity of compensation while the third generation cotton bollworm occurring. The investigations of damage number by larvae showed that the damage equivalent of the 1st-6th instar larval were 0.02, 0.14, 0.31, 0.47, 0.84 and 1.54 old bolls respectively. Based on direct and indirect benefits from controlling, the economic threshold of the third generation of the bollworm was worked out to be 26 accumulated eggs per 100 plants.

Agriculture↗

PubMed2001

[An new filling material of hair for the application of plastic surgery].

OBJECTIVE: To investigate the possibility of hair as a filling material for reconstructive or cosmetic surgery. METHODS: Sterilized pellets of hairs and silicone were selected and implanted subcutaneously in white rabbits. The clinical trial was carried out in 3 cases. RESULTS: A thin fibrous capsule, with much few inflammatory cells, was formed around the implanted hair pellets in 3-4 months. The inflammatory cells disappeared in the 9th month. No implant rejection, granulomatous formation or hair degradation was showed. CONCLUSION: Hair may be a valuable filling material for augmentation of the soft tissue.

Adult↗

PubMed2001

[Study on structure change and hypermethylation of p16 gene in multiple myeloma].

OBJECTIVE: To illustrate the role of structure and hypermethylation of p16 gene in the pathogenesis of multiple myeloma(MM). METHODS: By using PCR-single strand conformation polymorphisms(PCR-SSCP) and methylation-specific PCR(MSP) techniques, the structure and hypermethylation status of p16 gene in MM cell lines and patients were analysed. RESULTS: Homozygous deletion of p16 exon 2 was found in KM3 cells. The completely methylated p16 gene and hypermethylation of CPG island were observed in U266, LP1 cell lines and 55.56% of MM patients. CONCLUSION: Methylation of p16 gene is important in the pathogenesis of MM and may provide a new drug target for the treatment of MM.

Aged↗

PubMed2001

[Thrombopoietic effect of recombinant human thrombopoietin gene transferred to mice mediated by electric pulse on normal and experimental thrombocytopenia mice].

OBJECTIVE: To investigate the thrombopoietic effect of recombinant human thrombopoietin (rhTpo) gene transferred by electric pulse to normal and experimental thrombocytopenia mice. METHODS: Eukaryotic high expressing plasmid pcDI/Tpo was constructed by gene recombinant technology. 200 microg of the recombinant plasmid was injected into quadriceps femoris muscle of normal and experimental thrombocytopenia mice. Six times of electric pulse at 100v, 1Hz, 40ms were given immediately after the injection.The expression of rhTpo gene and its protein were assayed by RT-PCR and Western Blotting, respectively. Serum Tpo concentration was assayed by ELISA method. RESULTS: The recombinant plasmid pcDI/Tpo was successfully constructed. The expression of mRNA and protein of rhTpo gene was detected in the skeletal muscle of mice after transfection. Serum Tpo level increased from 328 +/- 89 ng/L to 1185 +/- 264 ng/L, and the platelet level of transfected mice increased from (259 +/- 27) x 10(9)/L to (640 +/- 31) x 10(9)/L. After injection with carboplatin, the platelet level decreased, but the nadir point was higher in pcDI/Tpo group than that in control group, and the recovery time of platelet count in pcDI/Tpo group shortened. CONCLUSION: The rhTpo gene could be effectively transfected to mice by electric pulse and played thrombopoietic role in vivo.

Animals↗

PubMed2001

[Regulatory effect of Bcl-2 on the cortical neurons of primary cultured mice with HSV-1 infection].

OBJECTIVE: To study the effect of Bcl-2 on the cortical neurons of primary cultured mice with HSV-1 infection. METHODS: Analysis of the expression of Bcl-2 on the cortical neurons of primary cultured fetal mice in vitro infected with HSV-1 for 11 hours or exposed to sorbitol for 5 hours was made by flow cytometry and Western blotting. RESULTS: The Bcl-2 of the cultured neurons undergoing HSV-1 infection expressed upregulating compared with the mock untreated neurons. The Bcl-2 protein of HSV-1 infected and exposed to sorbitol neurons expressed upregulating compared with the control group. The Bcl-2 protein of the primary cultured 3 day cells expressed higher than that of the primary cultured 7 day cells. The higher neurovirulence, the higher expression of Bcl-2. CONCLUSIONS: Bcl-2 might protect the primary cultured cortical neurons of fetal mice from apoptosis where infected with HSV-1, and thereby the lifespan of host cells may be prolonged.

Animals↗