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D W Warnock

Publications and source records attributed to D W Warnock.

At least 145 records · Page 8Linked to original sources
PubMed1982

Antifungals today.

Explore the source record for details and available documents.

Antifungal Agents↗
PubMed1982

Rapid enzyme-linked immunosorbent assay (ELISA) for Aspergillus fumigatus antibodies.

A rapid enzyme-linked immunosorbent assay (ELISA) where component incubation periods were shortened to one hour, was compared with agar gel double diffusion (AGDD) and a standard ELISA procedure for detecting antibodies to Aspergillus fumigatus in 28 asthmatic patients with suspected allergic aspergillosis. Using two A fumigatus antigens the rapid ELISA compared well with AGDD and the standard ELISA method. Eleven sera that reacted with both antigens in AGDD were all positive against antigen 1 in both forms of ELISA, but two failed to react with antigen 2 in the standard ELISA and three failed to react with this antigen in the rapid method. Thirteen AGDD-negative sera were also negative in both forms of ELISA. The rapid ELISA provides a sensitive and reproducible test for routine serological investigation of allergic aspergillosis.

Antibodies, Fungal↗
PubMed1982

Rapid serological diagnosis of Aspergillus fumigatus infection of the frontal sinuses and nasal chambers of the dog.

Aspergillus fumigatus precipitins in dog sera were detected by counterimmunoelectrophoresis (CIE). The procedure gave results within 90 minutes compared with 72 to 96 hours required in agar-gel double diffusion. Culture-filtrate antigens from two-week-old cultures of A fumigatus and two-day-old mycelial antigens produced the strongest reactions in CIE tests and the former antigen also revealed high titres in tests with serum from seven of 14 dogs with A fumigatus precipitins. Serum from these 14 dogs also reacted in CIE tests with a number of Penicillium species antigens.

Animals↗
PubMed1981

Further evaluation of indirect immunofluorescence methods for detection of antibodies against Aspergillus fumigatus.

The IF titres of IgG antibodies to hyphal antigen preparations of 6 isolates of Aspergillus fumigatus were compared with the titres to germling antigen preparations of the same isolates. Titres varied according the antigen used and the method of preparation. No single isolate or method of antigen preparation enabled a group of 15 patients with aspergillosis to be separated from a group of asthmatic subjects or a group of patients with other fungal infections, but significantly higher means IF titres were obtained for the group of patients with aspergillosis.

Antibodies, Fungal↗
PubMed1979

Antibodies to Candida species after operations on the large intestine: observations on the association with oral and faecal yeast colonization.

The occurrence of agglutinating and precipitating antibodies to Candida species was determined in 50 patients undergoing operations on the large intestine. The number of patients with oral or faecal yeast colonization and the amount of faecal colonization increased during the period after operation. No patient developed overt fungal infection but 15 patients developed precipitins to mannan antigens and 9 patients developed percipitins to cytoplasmic antigens during this period. The association between the formation of precipitins and the extent of faecal colonization is discussed.

Adult↗
PubMed1979

Epidemiological investigation of patients with vulvovaginal candidosis. Application of a resistogram method for strain differentiation of Candida albicans.

The resistogram method was applied to 420 isolates of Candida albicans obtained from 30 selected patients undergoing treatment for vulvovaginitis. Of these, 16 patients each harboured a particular strain of C. albicans which persisted in the mouth or intestinal tract or both. In three of these patients, this strain persisted in the genital tract, and, in eight patients, it later recolonised the genital tract. Fourteen patients harboured more than one strain of C. albicans: one failed to respond to treatment and continued to harbour the same strain in the genital tract; in five the original strain later recolonised the genital tract and a second strain remained confined to the mouth or intestinal tract or both; and in three a second strain, present in the intestinal tract, later colonised the genital tract. Each of the male partners of seven patients harboured a strain of C. albicans that was identical to the strain, or to one of the strains, that had been isolated from his female partner.

Adolescent↗
PubMed1979

Effect of simultaneous oral and vaginal treatment on the rate of cure and relapse in vaginal candidosis.

One hundred patients with vulvovaginal candidosis were entered in a double-blind trial to compare the effect of six days' local treatment with clotrimazole with that of the same treatment plus 10 days' oral treatment with nystatin. No significant differences were detected in the rate of cure or relapse between the treatment groups. The cure rate was lowest and the relapse rate highest in patients in whom vaginal candidosis had last been diagnosed during the preceding 12 months.

Administration, Oral↗
PubMed1978

Immunoglobulin classes of human serum antibodies in vaginal candidiasis.

The titre and immunoglobulin class of antibodies against Candida albicans in serum from 60 non-pregnant women was determined. IgG titres up to 1 : 32, IgA titres up to 1 : 8, and IgM titres up to 1 : 4 were detected in 30 women with vaginal candidiasis. Similar titres were found in 20 women harbouring yeasts in the mouth or rectum, and in 10 women who were not harbouring yeasts in the vagina, mouth or rectum. Serum fractionation confirmed that antibodies to C. albicans are found in the three immunoglobulin classes and that those antibodies reside in highest titre in the IgG class. No secretory IgA antibodies against C. albicans were detected in the serum of these women.

Antibodies, Fungal↗
PubMed1977

Grass pollen content of the air in the Bristol Channel region in 1976.

The grass pollen content of the air at four sites bordering the Bristol Channel (at Bristol, Cardiff, Penarth and Penmaen) was studied. The trap at Penmaen was situated at an official climatological station which provided the meteorological data used in the surveys. Although at certain times large differences could be seen between the concentrations of grass pollen at the different sites, overall the findings for the four sites were similar. Changes in the atmospheric pollen concentration related to variations in meteorological conditions. At the peak of the season high concentrations of grass pollen persisted throughout the night.

Atmosphere↗
PubMed1977

Observations on the use of the double diffusion test in the diagnosis of vaginal candidiasis.

Precipitins to mannan and cytoplasmic antigens of three Candida species were determined in an unselected series of 289 non-pregnant women. Precipitins were present in 20% of sera of women with vaginal candidiasis, in 23% of women harbouring yeasts in the vagina without clinical signs of infection, and in 21% of women harbouring yeasts in sites other than the vagina. Of the 47 patients who reacted with Candida albicans mannan or cytoplasmic antigens, 98% reacted with mannan antigen but only 13% with cytoplasmic antigen. The inclusion of mannan and cytoplasmic antigens of C. guilliermondii and C. parapsilosis did not increase the specificity or sensitivity of the test in the diagnosis of vaginal candidiasis. It is suggested that the double diffusion test is of doubtful value as an adjunct to the diagnosis of vaginal candidiasis.

Adolescent↗