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Biomedical subjects

D W Dresser

Publications and source records attributed to D W Dresser.

13 recordsLinked to original sources
PubMed1979

Restricted heterogeneity of antibody synthesized by T-cell deprived mice.

Mice which had been thymectomized and injected with anti-thymocyte serum to remove long-lived recirculating T cells, initially failed to produce haemagglutinating and haemolysing antibody after injection of sheep erythrocytes. After six fortnightly injections of heterologous erythrocytes, however, haemolysin titres in the T-cell deprived mice were comparable to those in similarly challenged but immunologically intact animals. Isoelectric focusing of these sera indicated that the anti-sheep erythrocyte antibody eventually synthesized by the T-cell deprived mice was less heterogeneous than antibodies found in the sera of control mice.

Animals↗
PubMed1979

IgM rheumatoid factor as a source of non-specificity in murine anti-allotype sera.

Anti-allotype sera have shown a breakdown of the expected specificity especially when used for the development of haemolysis of fragile target erythrocytes. IgM anti-IgG rheumatoid factor has been shown to be the source of non-specificity. Removal or destruction of IgM in an anti-allotype serum restores the expected specificity.

Animals↗
PubMed1979

The influence of T cells on the initiation and expression of immunological memory.

Nude and normal CBA mice have been used in adoptive transfer experiments to analyse the development of immunological memory. The development of B-cell memory to xenogeneic erythrocyte antigens is to a very large degree dependent on the presence of T cells, with IgG memory being somewhat more dependent than IgM memory. In this system, the expression of B memory, that is the transformation of memory cells to antibody-secreting cells under the inductive influence of antigen, is largely dependent on the presence of T cells. Primed (educated) T cells can have an antigen-specific potentiating effect on unprimed B cells in the presence of antigen.

Animals↗
PubMed1978

The effect of the parenteral administration of a rabbit anti-(mouse)-IgD serum on the immune response of mice to sheep erythrocytes.

Experiments have been carried out to find out if the administration of an anti-IgD serum to mice interferes in anyway with their immune response to sheep red blood cells. This was done to test a hypothesis that a biological role for IgD might be as a critical cellular receptor for antigen. Our results show that the injection of anti-IgD two days before antigen results in suppression of primary responses and priming (the antigen dependent generation of memory cells) but has no suppressive effect on a secondary response. On this indirect evidence we conclude that it is likely that IgD is present on antigen-sensitive percursor cells but not on memory cells.

Animals↗
PubMed1977

Conditions for the development of IgM- and IgG-antibody-secreting cells from primed mouse splenocytes in vitro.

A modified Marbrook chamber has been devised and manufactured which will fit inside a standard tissue culture Petri dish. A comparison has been made between Mishell-Dutton and modified Marbrook culture systems with respect to their ability to support an immune response by quiescent memory cells exposed to antigen in vitro. Good culture conditions are maintained for considerably longer in the modified Marbrook cultures and it seems that as a consequence these cultures support a large and reliable IgG response, normally absent or very small in cultures of the Mishell-Dutton type. Ease of manipulation and convenient size make the modified Marbrook system a good prospect for future long-term experiments.

Animals↗
PubMed1977

The immunoglobulin class of anti-hapten antibody secreted during secondary responses in vitro and in vivo.

A comparison has been made of the in vitro and in vivo response of primed mouse spleen cells to the hapten DNP. The responses were analysed in terms of six classes (sub-classes) of humoral antibody directed against the cross-reacting hapten TNP. By comparison with the response in intact mice the adoptive secondary response is delayed by 3 days in addition to being somewhat lesser in magnitude. The timing of the response in vitro is similar to that observed in intact mice. The preponderant class in all three responses was gammaG1 with gammaA and gammaG3 secreting cells consistently comprising the smallest proportion of the total of antibody-secreting cells.

Animals↗
PubMed1976

The immunological properties of haptens coupled to thymus-independent carrier molecules. IV. The IgG response to dinitrophenylated Ficoll.

Dinitrophenylated polysucrose (DNP-Ficoll) elicits T cell-independent IgM anti-DNP antibody formation in mice. This antigen also elicits a heterogeneous IgG1 and IgG2 anti-DNP response, which is operationally as T-independent as the IgM response. However, a concomitant graft-versus-host reaction markedly enhances the IgG response (allogeneic effect). These results confirm those of others, indicating that a certain proportion of the precursors of IgG-producing cells can be triggered by some T-independent antigens. However, our results suggest that even with such antigens optimal triggering of IgG precursors requires T cell help.

Animals↗
PubMed1975

The immune response of mice to phiX174. The potentiation of B-cell immunity and the suppression of T-cell help by pertussis vaccine.

The primary and secondary responses to phiX174 have been studied in T cell-deprived (T- minus) and control CBA mice including sham-operated (T+) and normal mice. The effect of pertussis on the immune response of these mice has been measured. It is concluded that: (1) the gammaG and memory responses to phiX are produced in T- minus mice even though greatly reduced in comparison with T+ and normal mice; (2) pertussis appears to enhance the gammaG response in T- minus mice while suppressing it in T+ and normal mice.

Adjuvants, Immunologic↗