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Biomedical subjects

D W Collins

Publications and source records attributed to D W Collins.

At least 19 recordsLinked to original sources
PubMed2002

Child clinical/pediatric neuropsychology: some recent advances.

The neuropsychological assets and deficits of several types of pediatric neurological disease, disorder, and dysfunction are described. These are examined from the perspective of the syndrome of nonverbal learning disabilities (NLD) and the "white matter model" designed to explain its complex manifestations. It is concluded that children with some of these diseases exhibit the NLD phenotype, whereas others do not. For the most part, the diseases in which the NLD phenotype is particularly evident are those wherein it has been demonstrated that perturbations of white matter (long myelinated fibers) are particularly prominent.

Asperger Syndrome↗
PubMed2002

The effects of sevoflurane on isolated gravid human myometrium.

The volatile anaesthetic agents are known to influence uterine muscle tone. All of the agents studied to date have been found to produce uterine relaxation. This property has been used to produce therapeutic uterine relaxation for difficult obstetric deliveries and the Ex Utero Intrapartum Treatment (EXIT) procedure. This study describes the effects of sevoflurane on isolated human myometrium at concentrations of 0.1, 0.25, 0.5, 0.75, 1.0, 1.5, 2.5 and 3.5 MAC. Sevoflurane produces dose-dependent depression of uterine muscle contractility with an ED50 of 0.94 MAC. Frequency of contraction was increased at concentrations of 2.5 MAC and greater. At concentrations of 3.5 MAC and above, uterine activity was virtually abolished.

Analysis of Variance↗
PubMed2002

Airway management on placental support (AMPS)--the anaesthetic perspective.

Neonatal airway obstruction has been reported to have a high mortality. Antenatal diagnosis of this condition is now possible. Anaesthetic and surgical techniques have been developed that allow neonatal airway obstruction to be managed at delivery, while the fetus remains oxygenated via the placental circulation. Three case studies are presented, and the anaesthetic issues for mother and fetus/neonate are discussed with reference to previously published cases of airway management on placental support. In particular, techniques for uterine relaxation and maintenance of placental circulation are explored. The history of these procedures and issues of planning and logistics are also discussed.

Adult↗
PubMed2000

Evidence for simultaneous protein interactions between human Rad51 paralogs.

In yeast, the Rad51-related proteins include Rad55 and Rad57, which form a heterodimer that interacts with Rad51. Five human Rad51 paralogs have been identified (XRCC2, XRCC3, Rad51B/Rad51L1, Rad51C/Rad51L2, and Rad51D/Rad51L3), and each interacts with one or more of the others. Previously we reported that HsRad51 interacts with XRCC3, and Rad51C interacts with XRCC3, Rad51B, and HsRad51. Here we report that in the yeast two-hybrid system, Rad51D interacts with XRCC2 and Rad51C. No other interactions, including self-interactions, were found, indicating that the observed interactions are specific. The yeast Rad51 interacts with human Rad51 and XRCC3, suggesting Rad51 conservation since the human yeast divergence. Data from yeast three-hybrid experiments indicate that a number of the pairs of interactions between human Rad51 paralogs can occur simultaneously. For example, Rad51B expression enhances the binding of Rad51C to XRCC3 and to HsRad51D, and Rad51C expression allows the indirect interaction of Rad51B with Rad51D. Experiments using 6xHis-tagged proteins in the baculovirus system confirm several of our yeast results, including Rad51B interaction with Rad51D only when Rad51C is simultaneously expressed and Rad51C interaction with XRCC2 only when Rad51D is present. These results suggest that these proteins may participate in one complex or multiple smaller ones.

Baculoviridae↗
PubMed2000

Esomeprazole improves healing and symptom resolution as compared with omeprazole in reflux oesophagitis patients: a randomized controlled trial. The Esomeprazole Study Investigators.

BACKGROUND: The pharmacologic profile of the new proton pump inhibitor esomeprazole has demonstrated advantages over omeprazole that suggest clinical benefits for patients with acid-related disease. METHODS: 1960 patients with endoscopy-confirmed reflux oesophagitis (RO) were randomized to once daily esomeprazole 40 mg (n=654) or 20 mg (n=656), or omeprazole 20 mg (n=650), the standard recommended dose for RO, for up to 8 weeks in a US, multicentre, double-blind trial. The primary efficacy variable was the proportion of patients healed at week 8. Secondary variables included healing and heartburn resolution at week 4, time to first resolution and sustained resolution of heartburn, and per cent of heartburn-free days and nights. Safety and tolerability were also evaluated. RESULTS: Significantly more patients were healed at week 8 with esomeprazole 40 mg (94.1%) and 20 mg (89.9%) vs. omeprazole 20 mg (86.9%), using cumulative life table estimates, ITT analysis (each P < 0.05). Esomeprazole 40 mg was also significantly more effective than omeprazole for healing at week 4 and for all secondary variables evaluating heartburn resolution. The most common adverse events in all treatment groups were headache, abdominal pain and diarrhoea. CONCLUSION: Esomeprazole was more effective than omeprazole in healing and symptom resolution in GERD patients with reflux oesophagitis, and had a tolerability profile comparable to that of omeprazole.

Adult↗
PubMed1998

Isolation and characterization of RAD51C, a new human member of the RAD51 family of related genes.

The yeast and human RAD51 genes encode strand-transfer proteins that are thought to be involved in both recombinational repair of DNA damage and meiotic recombination. In yeast, the Rad51 family of related proteins also includes Rad55, Rad57 and Dmc1. In mammalian cells, five genes in this family have been identified (HsRAD51, XRCC2, XRCC3, RAD51B/hREC2 and HsDMC1), and here we report the isolation of the sixth member, RAD51C. RAD51C was originally identified by a computer screen of the EST database. A full-length approximately 1.3 kb cDNA clone has been isolated that encodes a protein of 376 aa, having a 18-26% aa identity with other human Rad51 family members. RAD51C includes a previously mapped sequenced-tagged site location near the end of chromosome 17q. The RAD51C transcript is expressed in various human tissues, with highest level of expression in testis, followed by heart muscle, spleen and prostate. Yeast two-hybrid experiments indicate that the Rad51C protein binds to two other members of the Rad51 protein family (Xrcc3 and Rad51B) but not to itself. These findings suggest that Rad51C may function similarly to the yeast Rad55 or Rad57 proteins, rather than as a Rad51 functional homolog.

Amino Acid Sequence↗
PubMed1997

A large sex difference on a two-dimensional mental rotation task.

Mental rotation tests require participants to identify rotated versions of a target stimulus. The Vandenberg Mental Rotations Test depicts rotations in 3-D space and typically yields one of the largest established cognitive sex differences favoring males. It is presently unclear whether this male advantage is related to the nature of rotations depicted in 3-D space or to the high level of difficulty of this task. The present study developed a new test depicting picture plane, or 2-D, rotations. When task difficulty within this 2-D test was varied, a male advantage as large as that seen on the Vandenberg test was found for the difficult component. These findings suggest that processing in 3 dimensions is not a necessary condition for a large sex difference on tests of mental rotation.

Adult↗
PubMed1994

Rates of transition and transversion in coding sequences since the human-rodent divergence.

Protein-coding sequences of 337 human genes were compared with those of homologous genes from rodent (mouse or rat). A composite alignment containing 477,189 nucleotide positions was constructed, and 21,570 amino acid replacements were inferred. The rates of transitional and transversional silent substitutions in fourfold degenerate sites are estimated as 1.71 x 10(-9) and 1.22 x 10(-9) site -1 year -1, respectively. Rates of substitutions in replacement sites, subject to selective constraints mediated by the genetic code, are lower, but also reflect a transitional bias. The amino acid exchange rejected least often during evolution is Asp/Glu, which is fixed at 30% the rate of transversions in silent sites. The most mutable amino acids in this survey are threonine and serine; serine coded by AGY is more mutable than serine coded by TCN. A scoring matrix for evaluating amino acid similarity was derived from this study.

Amino Acid Sequence↗
PubMed1994

Removal of corneal foreign bodies: an instructional model.

A simple method for inflicting corneal foreign body injuries on bovine eyes is described and illustrated. The subsequent use of these eyes for teaching corneal and rust-ring removal is demonstrated. This method is suitable for instruction of large numbers of students and could be included in an undergraduate curriculum.

Animals↗
PubMed1993

Relationship between G + C in silent sites of codons and amino acid composition of human proteins.

We have investigated the relationship between the G + C content of silent (synonymous) sites in codons and the amino acid composition of encoded proteins for approximately 1,600 human genes. There are positive correlations between silent site G + C and the proportions of codons for Arg, Pro, Ala, Trp, His, Gln, and Leu and negative ones for Tyr, Phe, Asn, Ile, Lys, Asp, Thr, and Glu. The median proteins coded by groups of genes that differ in silent-site G + C content also differ in amino acid composition, as do some proteins coded by homologous genes. The pattern of compositional change can be largely explained by directional mutation pressure, the genetic code, and differences in the frequencies of accepted amino acid substitutions; the shifts in protein composition are likely to be selectively neutral.

Amino Acids↗
PubMed1993

FISH: a guide to protein-coding DNA sequences in the GenBank database.

FISH (Fast Index Search for Homologous coding sequences) consists of a database and associated software and is intended to function as a directory of protein-coding gene sequences. The FISH index contains descriptions of 22,361 DNA sequences from release 69.0 of the GenBank genetic sequence database. Complete coding sequences are represented numerically with counts of nucleotides and synonymous codons, and with GenBank LOCUS names and short descriptions. The software permits the database to be queried by GenBank LOCUS name, sequence length (expressed as total number of codons), or by comparison with a DNA sequence. In the latter case, the numerical descriptions are compared with simple distance measures in place of actual DNA sequences. The FISH package can be used to rapidly assemble lists of similar coding sequences, without regard to functional annotation or sequence alignments. Typical search times are well under a minute on widely available IBM-compatible microcomputers.

Algorithms↗
PubMed1992

Numerical classification of coding sequences.

DNA sequences coding for protein may be represented by counts of nucleotides or codons. A complete reading frame may be abbreviated by its base count, e.g. A76C158G121T74, or with the corresponding codon table, e.g. (AAA)0(AAC)1(AAG)9 ... (TTT)0. We propose that these numerical designations be used to augment current methods of sequence annotation. Because base counts and codon tables do not require revision as knowledge of function evolves, they are well-suited to act as cross-references, for example to identify redundant GenBank entries. These descriptors may be compared, in place of DNA sequences, to extract homologous genes from large databases. This approach permits rapid searching with good selectivity.

Animals↗
PubMed1992

A retinitis pigmentosa register for western Australia.

This paper describes the main elements of the Western Australian retinitis pigmentosa register including details of the data stored on the register, aspects of the coding systems used and some description of the tests employed in diagnosis of retinitis pigmentosa. The register is family based and contains data on affected individuals and on their unaffected relatives. As at November 1991, the register contained data for 391 individuals from 207 separate families. Of the 391 individuals, 240 had definite or probable retinitis pigmentosa and 26 were possibly affected. The remainder were unaffected family members. In many cases, both affected and unaffected family members are being studied serially and the register is designed to store and easily retrieve serial data to allow study of disease progression for individuals and within families.

Australia↗
PubMed1990

Thirteen years longitudinal study of computed tomography, visual electrophysiology and neuropsychological changes in Huntington's chorea patients and 50% at-risk asymptomatic subjects.

The aim of the study was to determine whether CT, visual electrophysiology and neuropsychological changes could assist with the detection of pre-clinical Huntington's chorea (HC). It was also hoped that the examination protocol would be of use in patients with early symptoms or signs of disease but with no family history of HC. Twenty-three patients with HC and 45 subjects at 50% risk, were studied serially. Each visit normally entailed a CT study without intravenous contrast, visual evoked response and eye movements studies, as well as a comprehensive psychiatric, neurological and neuropsychological assessment. Over the 13 years of the study 5 persons, initially symptom free on clinical grounds, developed HC. Of these, 3 had had early atrophy of the caudate nucleus on the first visit and 4 had shown abnormalities in the visual electrophysiology before becoming symptomatic. It is concluded that CT, visual electrophysiology and neuropsychological assessment may assist in the pre-clinical diagnosis of the at-risk HC subject and in the management of the early clinical case.

Adult↗
PubMed1985

Predicted early failure of cardiac pacemakers.

This paper describes a study on pacemaker longevity carried out on 65 subjects using mostly the Telectronics model 171 and model 173 pacemakers and based on an analysis of pacing rate reduction as a function of time since implant. Three different modes of rundown were observed. The shortest predicted lifetime was 23 months for a model 171 unit. This study was undertaken after the manufacturer and battery companies clearly delineated the existence of the problem and steps to be taken in the follow-up of patients. Careful analysis of medical records of the patients did not yield any correlation between sex, age, indications for implantation, initial pacing threshold, or amount of use of the pacemaker, and the early rundown of these pacemakers. Our study indicated that, in general, the model 173 had a shorter expected lifetime than the model 171. Using a reduction in pacing rate of 5.5 bpm from the rate at implant as the end-of-life indicator, the study showed that 16% of the model 173 pacemakers would need to be replaced within 3 years of implant and 78% within 6 years of implant. In the case of the model 171 units, 10% would need to be replaced within 3 years of implant and 48% within 6 years. Follow-up of patients with these units should be scheduled to monitor closely the rate of rundown in order to predict replacement dates.

Adult↗