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Biomedical subjects

D Vucković

Publications and source records attributed to D Vucković.

At least 19 recordsLinked to original sources
PubMed1998

Primary Campylobacter jejuni infection in different mice strains.

Campylobacter jejuni is one of the most frequent causes of diarrhoea in man. Extra-intestinal manifestations may also occur, particularly in immunocompromised individuals. However, because of the lack of appropriate animal models the pathophysiology and immunological response of the host to C. jejuni infection are still poorly understood. In our laboratory an experimental infection of adult BALB/c, C57BL/6 and DBA/2 mice has been established. After intraperitoneal injection of 0.5-1x10(9) cfu of C. jejuni none of the infected mice showed clinical symptoms of illness, but bacterial spreading and tissue invasion were achieved. We have concentrated our studies on the duration of primary infection, recovery of bacteria from livers and spleens of infected animals and pathohistological changes of these organs. Our results showed differences in the course of systemic infection among the tested mice strains. BALB/c mice were most sensitive, resulting in the most pronounced pathohistological changes in the examined organs. The duration of the primary liver infection was the longest in BALB/c mice while the duration of the splenic infection also differed among the tested mice strains. Nevertheless, the experimental model used in this study can be efficiently used in further analysis of the pathogenesis of this bacterial infection. However, the strain differences should be taken into account depending on the parameters to be followed.

Animals↗
PubMed1997

Changes in macrophage function during chemotherapy.

Antibiotics, in addition to killing or inhibiting the growth of microorganisms, may also affect the mechanism of host defence in many ways. Such effects may be clinically relevant especially in the case where an impairment of immunological function can be seen. We, therefore, decided to study the influence of penicillin G, cefotaxim, ceftazidime, streptomycin, and lincomycin on the function of phagocytes by using the macrophage adherence assay and the macrophage spreading assay. We also followed the concentrations of neopterin and interferon gamma (IFN gamma) in the plasma of mice treated with the above mentioned antibiotics. Changes in adherence of peritoneal macrophages were seen in mice treated with therapeutic doses of penicillin G and cefotaxim, after 2 h of incubation. Cefotaxim and streptomycin in the usual therapeutic dose and ceftazidime in a fourfold higher dose influenced the capacity of peritoneal macrophages to spread on a glass surface. The same was seen with lincomycin when administered in the therapeutic dose and in a fourfold higher dose. In all the mice treated with antibiotics the concentration of IFN gamma was higher than in the control mice, while the reverse was seen concerning neopterin release, with an exception in mice treated with streptomycin.

Animals↗
PubMed1996

[Atherosclerotic plaque hemorrhage as one form of a complicated atherosclerotic lesion].

Atherosclerotic plaque hemorrhage is a form of a complicated atherosclerotic lesion. It is a finding of extravasation of erythrocytes (fresh hemorrhage) or siderophages (old hemorrhage) in the defined atherosclerotic changes. 510 segments of the main epicardial coronary arteries (right coronary artery, anterior interventricular branch of the left coronary artery, circumflex branch of the left coronary artery) were microscopically examined in 50 obduction cases of patients with ischemic heart diseases. Fresh hemorrhage in the atherosclerotic plaque was established in 25 cases, that is in 40 microscopically examined coronary artery segments. It was established in all cases where there was a atherosclerotic plaque rupture, in some segments where process of thrombotic organization occurred, as well as in some segments of arteries which supply the region of early myocardial infarction. All hemorrhages occur inside lipidic and fibrolipidic atherosclerotic changes of intima. The average length of fresh hemorrhage was 0.8 cm. 92.50% of segments with fresh hemorrhages occur in the first 4 cm of the examined arteries. 88% of plaque hemorrhages occur in segments with 76-100% lumen obstruction. Signs of old hemorrhages most often occur in segments with 51-75% and 76-100% lumen obstruction. Siderophages mostly occur in complicated atherosclerotic changes and thrombotic organization.

Coronary Artery Disease↗
PubMed1996

[Calcification in atherosclerotic plaque as one of the types of complicated atherosclerotic lesions].

Calcifications of the atherosclerotic plaque is the form of a complicated atherosclerotic lesion. It is characterized by granular or massive deposits of calcium chloride inside the atherosclerotic plaque. It was found in at least one of the three main coronary arteries in 43 (86.00%) out of 50 autopsy cases. In all three, coronary arteries calcifications were found in 8 (18.60%) cases, in two coronary arteries in 19 (44.19%), and one coronary artery in 16 (37.21%) cases. Calcifications of atherosclerotic changes which contain fats were statistically significantly more frequently established than those which did not contain fats. In three fourths of cases calcifications were present in segments with significant narrowing of the arterial lumen (group 3 and 4). In 59.61% of calcificated atherosclerotic changes the residual lumen was of eccentric shape, in 20.51% of concentric shape, in 7.69% it was semilunar, and in 12.18% a few lumens were detected (channeled and organized thrombi). Granular deposits of calcium were established 63.46%, while massive in 36.54% of segments of atherosclerotic lesions, but no statistically significant difference in distribution of these two types of calcifications was established in regard to the type of atherosclerotic change.

Aged↗
PubMed1995

[Pathohistologic changes in aortocoronary venous grafts].

This paper analyses histological changes of venous grafts in 52 obduction cases who had undergone aortocoronary bypass grafting and in whom death occurred after more than 30 days from surgery. The group consisted of 46 males and 6 females. The mean age at the moment of surgery was 51.5, while on the average the patients lived 4.83 years after the surgery. 144 venous grafts were implanted, that is 2.77 per patient. In 49 (34.03%) venous grafts fibrosis intima was established. As in 43 (29.86%) grafts it did not cause occlusion of the lumen greater than 25%, these kinds of grafts were marked as unchanged, normal. In 43 (29.00%) grafts atherosclerosis was established, in 14 (9.72%) fibrointimal proliferation, and in 38 (26.39) lumen obliteration. A year after the surgery the most important pathohistologic change was fibrointimal proliferation, and two years after the surgery and on-atherosclerosis. The percentage of certain pathohistologic types of graft changes, no matter how long patients survived after the surgery was the same in both patients with implants of the coronary artery and those with anterior interventricular branch of the left coronary artery. Grafts implanted into branches of the circumflex artery were statistically significantly more often obliterated. The locality of anastomosis of the venous graft and artery was not occluded in 67.31% of all obduction cases, whereas it was occluded in 32.69% of cases or 32 (22.22%) implanted grafts. Occlusions were caused by atherosclerotic change of the anastomosis in 84.37% of grafts, and by simultaneous occurrence of atherosclerotic change and thromb in 15.63% of grafts.

Coronary Artery Bypass↗
PubMed1995

[Analysis of the cause of death in patients with aortocoronary bypass implants].

This paper analyzes causes of death in 91 autopsied patients in whom aortocoronary bypass surgery was performed as a therapy of ischemic heart disease. Patients who died within 30 days after surgery made up the group of so-called "early deaths", while those who died 30 or more days after the surgery made up the group of so-called "late deaths". The leading cause of death in the group of "early deaths" was myocardial infarction (51%) and then causes of death connected to other systems of organs (38%). Hematopericardium and consequential cardiac tamponade was the cause of death in 8% of cases, while in 3% of cases it was cardiac decompensation. In the group of "late deaths" the most important cause of death was myocardial infarction (56%), and then cardiac decompensation (27%), as well as causes of death connected to other systems of organs (17%).

Cardiac Tamponade↗
PubMed1995

[Correlation of postmortem coronarography and the macroscopic and microscopic determination of luminal narrowing of coronary arteries in ischemic heart disease].

In our work we used 30 consecutive abduction cases of individuals suffering from ischemic heart disease. Postmortal coronarography was performed in all and we examined the narrowness of artery lumen, its degree, existence and locality of the obturatory thrombus, artery domination, state of aortocoronary grafts and the existence of anastomoses. Coronary arteries were macroscopically examined by cuts on every 0.5 cm of the established aims, and then segments were examined microscopically. The gathered results were compared and the degree of agreement of the two methods was calculated. An agreement of 100% was established when determining artery domination and presence of obturatory thrombus, although the number of found thrombi was bigger when macroscopic/microscopic examination was done. The defined degree of artery lumen narrowness was identical in 75% of cases.

Aged↗
PubMed1994

[Types of atherosclerotic changes in relation to the type of residual lumen and degree of narrowing in coronary arteries in ischemic heart disease].

510 segments of all three coronary arteries in 50 obduction cases of individuals suffering from ischemic (coronary) heart disease were microscopically examined. We examined the type of atherosclerotic change, the degree of narrowness of lumen artery and the type of residual lumen. In the group one of the narrowness of artery lumen 44 segments with fibrotic plaque were found, where concentric shape of lumen dominates. In the group two of the narrowness 176 segments were found. Most of them were with fibrotic plaque, but complicated atherosclerotic lesions were also present. According to the shape of residual lumen the concentric narrow shape dominates. In the group three of the narrowness of the lumen 191 segments were found; considering the type of atherosclerotic change complicated lesions were most common, while lipidic plaque was also present. An eccentric shape of residual lumen dominates this group. In the group four of the narrowness of lumen artery 99 segments were found; in this group we found fibrotic plaque and complicated atherosclerotic lesions. The most common finding of the residual lumen is the type of multiple lumen (channelled thrombi).

Coronary Artery Disease↗
PubMed1991

[Familial hemophagocytic lymphohistiocytosis].

The paper presents two cases of familial hemophagocytic lymphohistiocytosis in one family. The first case is the first-born female child with symptoms of an abrupt onset of hepatic failure and encephalopathy at the age of 2.5 months, and the second case is the third-born male child from the fourth pregnancy who died in his first month with a similar clinical manifestation. By means of histological examination, a diffuse proliferation of histiocytes of benign appearance with numerous phagocytized erythrocytes in the cytoplasm was found in both patients. These infiltrates existed in numerous organs and tissues of the body, but they were mostly visible in the spleen, liver, lymph nodes and in the bone marrow. The course of the disease is rapidly progressive with an up to now insufficiently explained etiology of the disease, with the existence of an autosomal recessive type of inheritance and defects in cellular and humoral immunity.

Female↗
PubMed1991

[Cystic teratoma of the neck].

Teratomas of the neck are very rare, and up to now only a small number of cases has been described (10 in adults), while in children it is a more frequent finding (132 described cases). It is judged that up to 4% of all teratomas in children are located in the cervicothyroid region. The paper presents a case of a cystic teratoma of the neck in a male child of 1.5 years of age, which gradually increased in it's size during the course of 12 months prior to operation. The tumor had the dimensions 3.7 x 3.2 x 2.7 cm, it was multicystic, and clinically it gave the impression of a bronchial cyst. Histologically the tumor contained elements of all three primary embryonic germ layers.

Dermoid Cyst↗
PubMedDate not supplied

Ultrastructural aspect of myocarditis: its relevance for the diagnosis.

The Dallas consensus was used to reveal active or borderline inflammatory loci by light microscopy (LM). When lymphocyte-cardiocyte interaction was observed by electron microscopy (EM), the deleterious or dormant pattern of inflammatory process was recognized. The first was determined by lymphocytes that adhered to cardiocytes, next to necrotic cardiocytes or admixed with debris. The second was marked by scattered lymphocytes between preserved cardiocytes and the absence of lymphocytes adhered to cardiocytes and necrotic cardiocytes. The deleterious pattern of the inflammatory process (EM) commonly supplemented the active appearance of inflammatory loci (LM). In contrast, the borderline outlook of the LM completed either the deleterious or dormant pattern of the EM. This discrepancy was related to the restricted resolution of LM, which might hide the actual stage of the disease. The diagnosis of myocarditis was founded on mutual LM and EM observations. The active or borderline appearance of LM of the deleterious pattern (EM) was considered indicative for the active stage of myocarditis. The borderline outlook of the LM of the dormant pattern of the EM was admitted to indicate either the healing phase of the disease with lymphocytes still lagging behind, or a latent phase of the ongoing myocarditis, according to the patient's hemodynamic status.

Biopsy↗
PubMedDate not supplied

Level of left ventricular dysfunction in endomyocardial biopsy-proven myocarditis.

The level of myocardial functional impairment in the course of myocarditis still remains associated with controversial data. However, many investigators agree that there is a significant heart failure during myocarditis and a large number of studies suggest development of global chronic or acute heart failure. The objective of this study was to establish haemodynamic parameters as descriptors of the level of myocardial systolic and diastolic impairment. From 131 investigated patients, 95 were assigned to the study group and 36 to the control group. All patients underwent right and left heart catheterization. A group of patients underwent EMB (endomyocardial biopsy) of the right ventricle. According to the Dallas Criteria, patients were divided into three groups: active myocarditis with fibrosis, active myocarditis without fibrosis and borderline myocarditis. The following haemodynamic parameters were evaluated: left ventricle EF (ejection fraction), cardiac index and end diastolic pressure of the left ventricle. EF, which reflects left ventricular systolic impairment, showed a difference related to the control group, while there was no difference in cardiac index between the groups. Diastolic dysfunction (end diastolic pressure of the left ventricle) was noted in the group with active myocarditis, but not in the group with borderline myocarditis.

Adult↗